上海交通大学：《微生物学 Microbiology》课程教学资源（双语课件）Lecture 5.1 Microbial Growth（Bacterial Cell Division、Growth of Bacterial Populations 细菌群体生长）

上泽充通大学 Shanghai Jiao Tong University Lecture 5-1 Microbial Growth CHAPTER 5 in BROCK BIOLOGY OF MICROORGANISMS Zhao Liping,Chen Feng School of Life Science and Biotechnology Shanghai Jiao Tong University http://micro.sjtu.edu.cn

Lecture 5-1 Microbial Growth Zhao Liping, Chen Feng School of Life Science and Biotechnology Shanghai Jiao Tong University http://micro.sjtu.edu.cn CHAPTER 5 in BROCK BIOLOGY OF MICROORGANISMS

上泽充通大学 Shanghai Jiao Tong University I.Bacterial Cell Division 5.1 Cell Growth and Binary Fission In microbiology,growth生长is defined as an increase in the number of cells. By definition,when one cell divides to form two, one generation世代has occurred. Shanghai Jiao Tong University

Shanghai Jiao Tong University I. Bacterial Cell Division 5.1 Cell Growth and Binary Fission In microbiology, growth生长is defined as an increase in the number of cells. By definition, when one cell divides to form two, one generation世代has occurred

Binary fission二分分裂 Cell elongation Septum Septum formation Completion of septum; formation of walls;cell separation Bacterial Growth 2012 Pearson Educalion.ino In most prokaryotes,growth of an individual cell continues until the cell divides into two new cells,a process called binary fission

Binary fission 二分分裂 In most prokaryotes, growth of an individual cell continues until the cell divides into two new cells, a process called binary fission. Bacterial Growth

上泽克通大学 Shanghai Jiao Tong University 5.2 Fts proteins and the cell division plane Fts proteins("filamentous temperature sensitive") are important in the cell division process of prokaryotes. Fts proteins are universally distributed among prokaryotes,including the Archaea,and FtsZ proteins have been found in mitochondria线粒体 and chloroplasts叶绿体.Fts蛋白广泛存在于原核生物 ©FtsZ shows structural similarities to tubulin微管蛋 the important cell division protein in eukaryotes. Shanghai Jiao Tong University

Shanghai Jiao Tong University 5.2 Fts proteins and the cell division plane Fts proteins (“filamentous temperature sensitive”) are important in the cell division process of prokaryotes. Fts proteins are universally distributed among prokaryotes, including the Archaea, and FtsZ proteins have been found in mitochondria线粒体 and chloroplasts叶绿体. Fts蛋白广泛存在于原核生物 FtsZ shows structural similarities to tubulin微管蛋 白, the important cell division protein in eukaryotes

圆上泽充廷大￥ Shanabai liao Tong lniversitu FtsZ ring Divisome Cytoplasmic proteins membrane Division plane In rod-shaped cells,formation of the divisome begins with the attachment of molecules of FtsZ in a ring around the cell cylinder in the precise center of the cel.细胞的“分裂装置”称为分裂体 (divisome),杆菌的分裂体形成于FtsZ环，该环位于细胞柱体的正中 央

Shanghai Jiao Tong University In rod-shaped cells, formation of the divisome begins with the attachment of molecules of FtsZ in a ring around the cell cylinder in the precise center of the cell. 细胞的“分裂装置”称为分裂体 (divisome), 杆菌的分裂体形成于FtsZ环, 该环位于细胞柱体的正中 央

Outer membrane The arrangement of Peptidoglycan individual divisome Ftsl FtsA Cytoplasmic proteins: ATP membrane ZipA is an FtsZ anchor; GTP GDP+ ADP FtsZ P Pi ring Divisome Ftsl is a peptidoglycan complex FtsZ ring biosynthesis protein; Cytoplasmic membrane FtsK assists in chromosome separation; FtsA is an ATPase. Phase-contrast Cells stained with a specific T.den Blaauwen Nanne Nanninga,reagent against FtsZ Univ.of Amsterdam () Binary fission 2012 Pearson Education Ine

The arrangement of individual divisome proteins: ZipA is an FtsZ anchor; FtsI is a peptidoglycan biosynthesis protein; FtsK assists in chromosome separation; FtsA is an ATPase. Phase-contrast Cells stained with a specific reagent against FtsZ Binary fission

上泽充通大￥ DNA replication,Min Proteins, and Cell Division Min CD Minutes Cell wall 0 质配会 Cytoplasmic Location of the actual membrane Nucleoid cell midpoint by FtsZ is 20 食Qe MinE facilitated by Min proteins,especially 40 MinC,MinD,and MinE. 顶暖6 Divisome complex 60 FtsZ ring Septum 80 同 瘦哑 Nucleoid MinE 2012 Pearson Education,Inc

Shanghai Jiao Tong University DNA replication, Min Proteins, and Cell Division Location of the actual cell midpoint by FtsZ is facilitated by Min proteins, especially MinC, MinD, and MinE

Figure 5.4 MreB and FtsZ Cell wall Determinants of Cytoplasmic Cell Morphology membrane MreB Sites of cell wall synthesis (a) (b) (c) Other kinds of division

Figure 5.4 Cell wall Cytoplasmic membrane MreB Sites of cell wall synthesis FtsZ MreB and Determinants of Cell Morphology Other kinds of division

上帝充通大学 Shanghai Jiao Tong University 5.3 Peptidoglycan synthesis and cell division 糖合成与细胞分裂 2000 chemical reactions in the cell division Energy transformations Biosynthesis of monomers,coenzymes,cofactors etc. Polymerization reactions-synthesis of polymers from monomers Shanghai Jiao Tong University

Shanghai Jiao Tong University 5.3 Peptidoglycan synthesis and cell division 肽聚 糖合成与细胞分裂 2000 chemical reactions in the cell division • Energy transformations • Biosynthesis of monomers, coenzymes, cofactors etc. • Polymerization reactions-synthesis of polymers from monomers

上游充通大学 FtsZ ring Shanghai Jiao Tong University 60827) Wall bands Growth zone (a) Septum 立 () 2012 Pearson Education,Ine Localization of new cell wall synthesis during cell division.In cocci, new cell wall synthesis(shown in green)is localized at only one point..球菌新合成的细菌壁（绿色部分）位于中间Shanghai Jiao Tong University

Shanghai Jiao Tong University Localization of new cell wall synthesis during cell division. In cocci, new cell wall synthesis (shown in green) is localized at only one point.球菌新合成的细菌壁(绿色部分)位于中间