上海交通大学：《微生物学 Microbiology》课程教学资源（双语课件）Lecture 9.2 Essentials of Virology（Viral Replication 病毒复制、Viral Diversity）

上游充通大 SHANGHAI JIAO TONG UNIVERSITY 1896 1920 1987 2006 Virus:Growth,quantification,replication and diversity Lecture 9-2 Essentials of Virology CHAPTER 9 in BROCK BIOLOGY OF MICROORGANISMS Chen FengNG School of Life Science and Biotechnology, Shanghai Jiao Tong University Email:cf2001@situ.edu.cn http://micro.sjtu.edu.cn

1896 1920 1987 2006 Lecture 9-2 Essentials of Virology Virus: Growth, quantification, replication and diversity CHAPTER 9 in BROCK BIOLOGY OF MICROORGANISMS Chen Feng School of Life Science and Biotechnology, Shanghai Jiao Tong University Email: cf2001@sjtu.edu.cn http://micro.sjtu.edu.cn

上游充通大￥ SHANGHAI JIAO TONG UNIVERSITY lI.Viral Replication病毒复制 9.4 General Features of Virus Replication 9.5 Virus Multiplication:Attachment and Penetration 9.6 Virus Multiplication:Production of Virual Nucleic Acid and Protein Chen Feng,Shanghai Jiao Tong University

Chen Feng, Shanghai Jiao Tong University II. Viral Replication病毒复制 9.4 General Features of Virus Replication 9.5 Virus Multiplication: Attachment and Penetration 9.6 Virus Multiplication: Production of Virual Nucleic Acid and Protein

上游充通大学 SHANGHAI JIAO TONG UNIVERSITY 9.4 General Features of Virus Replication Phases of Viral Replication Attachment(adsorption)of the virus to a susceptible host cell Entry (penetration)of the virion or its nucleic acid Synthesis of virus nucleic acid and protein by cell metabolism as redirected by virus Assembly of capsids and packaging of viral genomes into new virions(maturation) Release of mature virions from host cell Chen Feng,Shanghai Jiao Tong University

Chen Feng, Shanghai Jiao Tong University 9.4 General Features of Virus Replication Phases of Viral Replication • Attachment (adsorption) of the virus to a susceptible host cell • Entry (penetration) of the virion or its nucleic acid • Synthesis of virus nucleic acid and protein by cell metabolism as redirected by virus • Assembly of capsids and packaging of viral genomes into new virions (maturation) • Release of mature virions from host cell

Figure 9.8 Virion DNA Attachment (adsorption) Cell (host) Protein coat remains outside Penetration (injection) Viral DNA enters Synthesis of nucleic acid and protein Assembly and packaging Release (lysis) Virions 2012 Pearson Education,Inc

Figure 9.8 Protein coat remains outside Viral DNA enters Virion DNA Cell (host) Virions Attachment (adsorption) Penetration (injection) Synthesis of nucleic acid and protein Assembly and packaging Release (lysis) © 2012 Pearson Education, Inc

Following adsorption,the Eclipse Maturation infectivity of the virus particles disappears,a Early Nucleic Protein phenomenon enzymes acid coats called eclipsel隐蔽 期 During the latent Virus period潜伏期， added Assembly replication of viral and nucleic acid and Latent period release protein occurs.The maturation period follows(virus nucleic acid and protein are Time assembled) Figure 9-9 Brock Biology of Microorganisms 11/e 2006 Pearson Prentice Hall,Inc. One-step growth curve一步生长曲线

One-step growth curve 一步生长曲线 Following adsorption, the infectivity of the virus particles disappears, a phenomenon called eclipse隐蔽 期. During the latent period潜伏期, replication of viral nucleic acid and protein occurs. The maturation period follows (virus nucleic acid and protein are assembled)

9.5 Virus Multiplication:Attachment and Penetration Example:T4 phage (a)Unattached virion. (b)Attachment to the (c)Contact of cell wall by the wall by the long tail tail pins尾针. fibers尾丝interacting with core polysaccharide. (d)Contraction of the tail sheath尾鞘and injection of the DNA. Lipopolysaccharide layer(outer membrane) Peptidoglycan Cytoplasmic membrane

9.5 Virus Multiplication: Attachment and Penetration (a) Unattached virion. (b) Attachment to the wall by the long tail fibers尾丝 interacting with core polysaccharide. (c) Contact of cell wall by the tail pins尾针. (d) Contraction of the tail sheath 尾鞘and injection of the DNA. Example: T4 phage

Figure 9.10 (a) (b) (c) Tail fibers Tail pins Outer 致级核攻攻及及员级级级级级领级 membrane Tail Peptidoglycan lysozyme Cytoplasmic membrane Cytoplasm T4 genome 2012 Pearson Education,Inc

Figure 9.10 Tail fibers Tail pins Outer membrane Peptidoglycan Cytoplasmic membrane Cytoplasm Tail lysozyme T4 genome © 2012 Pearson Education, Inc

START RECEPTORS LIGANDS OR DOCKING PROTEINS 季 PENETRATION HHHH-HH-HHH DOCKING/ADSORPTION herpesviruses,paramyxoviruses,HIV Attachment and penetration of Animal virus

Attachment and penetration of Animal virus

上游充通大学 SHANGHAI JIAO TONG UNIVERSITY Uptake of an enveloped virion by an animal cell (a)Direct penetration by naked viruses Caps ol-ol-d b)Enveloped virs 元0 c)Entry of enveloped virus by endocytosis ⑨o@@d. Figure 18.4 Animal Virus Entry.Mechanisms of animal virus attachment and entry into host cells.See text for description of the three entry modes

Chen Feng, Shanghai Jiao Tong University Uptake of an enveloped virion by an animal cell

上游充通大学 SHANGHAI JIAO TONG UNIVERSITY 9.6 Virus Multiplication:Production of Virual Nucleic Acid and Protein Viruses have different types of genomes,including double-stranded/single-stranded DNA and double- stranded/single-stranded RNA. Chen Feng,Shanghai Jiao Tong University

Chen Feng, Shanghai Jiao Tong University 9.6 Virus Multiplication: Production of Virual Nucleic Acid and Protein Viruses have different types of genomes, including double-stranded/ single-stranded DNA and double￾stranded/ single-stranded RNA