上海交通大学：《微生物学 Microbiology》课程教学资源（双语课件）Lecture 3 Microbial Cell Biology（3.4）Surface structures and inclusions of prokaryotes

上充通大学 Shanghai Jiao Tong University Cell Structure and Function Lecture 3-4: Microbial cell biology Zhao Liping,Chen Feng School of Life Science and Biotechnology Shanghai Jiao Tong University http://micro.sjtu.edu.cn

Lecture 3-4: Microbial cell biology Cell Structure and Function Zhao Liping, Chen Feng School of Life Science and Biotechnology Shanghai Jiao Tong University http://micro.sjtu.edu.cn

上充通大学 Shanghai Jiao Tong University Surface structures and inclusions of prokaryotes 4.10 Bacterial cell surface structures 4.11 Endospores内生孢子

III. Surface structures and inclusions of prokaryotes 4.10 Bacterial cell surface structures 4.11 Endospores 内生孢子

h 上游充通大￥ Shanghai Jiao Tong University 4.10 Bacterial cell surface structures Glycocalyx糖被：slimy or gummy materials on prokaryotic cell surfaces,consist of polysaccharide (sometimes protein). Shanghai Jiao Tong University

Shanghai Jiao Tong University Shanghai Jiao Tong University 4.10 Bacterial cell surface structures Glycocalyx 糖被: slimy or gummy materials on prokaryotic cell surfaces, consist of polysaccharide (sometimes protein)

Capsule荚膜 Glycocalyx can be divided into 1)capsule(macrocapsule), 2)microcapsule,3)slime layer and 4)zoogloea depending on their rigidity and thickness.糖被可分为荚膜（大荚膜）、微荚膜、粘液 层和菌胶团

Capsule荚膜 Glycocalyx can be divided into 1)capsule (macrocapsule), 2)microcapsule, 3)slime layer and 4)zoogloea depending on their rigidity and thickness.糖被可分为荚膜（大荚膜）、微荚膜、粘液 层和菌胶团

The rigid layers are organized in a tight matrix that excludes particles,this form is capsule;If the glycocalyx is more easily deformed,it will not exclude particles,this arrangement is slime layer..荚膜与粘液层的不同

The rigid layers are organized in a tight matrix that excludes particles, this form is capsule; If the glycocalyx is more easily deformed, it will not exclude particles, this arrangement is slime layer.荚膜与粘液层的不同

上充通大学 Shanghai Jiao Tong University Discuss:Any advantages of glaycocalyx

Discuss: Any advantages of glaycocalyx ?

h 上降充通大睾 Shanghai Jiao Tong University 4.11 Endospores内生孢子 Properties of Endospores Structure of Endospores Chemical Constitution of Endospores Shanghai Jiao Tong University

Shanghai Jiao Tong University Shanghai Jiao Tong University 4.11 Endospores 内生孢子 Properties of Endospores Structure of Endospores Chemical Constitution of Endospores

上浒充通大学 Shanghai Jiao Tong University Endospores Endospores:differentiated cells that are very resistant to heat and cannot be destroyed easily, even by harsh chemicals. Stay alive for millions of years Morphological properties ·Difficult to stain Highly refractile body ©内生孢子：1、对热和化学物质有高度抗性；2、可以存 活上百万年；3、在形态学上不易染色并且有高度的折光氏 性； Shanghai Jiao Tong University

Shanghai Jiao Tong University Shanghai Jiao Tong University Endospores Endospores: differentiated cells that are very resistant to heat and cannot be destroyed easily, even by harsh chemicals. Stay alive for millions of years Morphological properties • Difficult to stain • Highly refractile body 内生孢子：1、对热和化学物质有高度抗性；2、可以存 活上百万年；3、在形态学上不易染色并且有高度的折光 性；

上游充通大学 司 Shanghai Jiao Tong University How long can a bacterial cell survive? In what form? Halophilic endospore-forming bacteria have been isolated from fluid inclusions in an 250- million-year-old salt crystal. (Nature,2000,407:897-900) 6 Shanghai Jiao Tong University

Shanghai Jiao Tong University Shanghai Jiao Tong University How long can a bacterial cell survive? In what form? Halophilic endospore-forming bacteria have been isolated from fluid inclusions in an 250- million-year-old salt crystal. (Nature, 2000, 407: 897-900)

4.11.1 Endospore Structure The outermost layer:exosporium 壁，athin,delicate covering made of Exosporium protein. Spore coat 因 Within exosporium:spore coats.芽胞 Core wall 衣，composed of layers of spore- Cortex specific proteins. DNA Below the spore coat::cortex皮层， which consists of loosely cross-linked peptidoglycan. 国 Inside the cortex:core or spore protoplast核/芽胞原生质体，which contains the usual cell wall(core wall), (a) cytoplasmic membrane,cytoplasm, 2012 Poarson Education.Inc. nucleoid,and so on

4.11.1 Endospore Structure The outermost layer: exosporium外 壁, a thin, delicate covering made of protein. Within exosporium: spore coats芽胞 衣, composed of layers of spore￾specific proteins. Below the spore coat: cortex皮层, which consists of loosely cross-linked peptidoglycan. Inside the cortex: core or spore protoplast核/芽胞原生质体, which contains the usual cell wall (core wall), cytoplasmic membrane, cytoplasm, nucleoid, and so on