华南师范大学：《植物生理学》课程教学资源（授课教案）生物科学研究 第五次课教案 如何报告科学研究成果

授课题目（教学章节或主题）：授课类型理论课授课时间第14周第1-3节如何报告科学研究成果教学目标或要求：使学生了解如何做墙报、参加国内外学术会议并口头报告研究成果教学内容（包括基本内容、重点、难点）：基本内容：1）为什么要报告研究成果？交流成果2）交流的形式国际学术会议和国内学术会议ConferenceMeetingSymposiumWorkshop3）报告形式口头报告（oralreport）墙报(post)1）如何准备口头报告准备：了解：发言时间、提问时间、视听设备等考虑：听众、内容、开场白、结束语写好：报告内容，提前准备可能的提问、试讲：把握好时间、分寸、注意仪表、形象等，不要读讲稿！改写讲稿：试讲之后根据大家提的意见修改发言稿2）如何准备墙报准备了解：墙报尺寸、展示板和粘贴方法、展示地点和时间

授课题目（教学章节或主题）： 如何报告科学研究成果 授课类型 理论课 授课时间 第14 周第1－3 节 教学目标或要求： 使学生了解如何做墙报、参加国内外学术会议并口头报告研究成果 教学内容（包括基本内容、重点、难点）： 基本内容： 1）为什么要报告研究成果？ 交流成果 2）交流的形式 国际学术会议和国内学术会议 Conference Meeting Symposium Workshop 3）报告形式  口头报告(oral report)  墙报（post) 1） 如何准备口头报告  准备：了解： 发言时间、提问时间、视听设备等 考虑： 听众、内容、开场白、结束语 写好： 报告内容，提前准备可能的提问  试讲： 把握好时间、分寸、注意仪表、形象等，不要读讲稿！  改写讲稿：试讲之后根据大家提的意见修改发言稿 2） 如何准备墙报  准备 了解：墙报尺寸、展示板和粘贴方法、展示地点和时间

设计：版面生动、吸引人布局：方便携带，容易布置标题：简明、具体。正文：句子简短，小标题适当，颜色合适。举例：老动提取道以游结合浓药出防治线虫的研究L实腔特E0006Q0Q9

 设计：版面生动、吸引人 布局：方便携带，容易布置 标题：简明、具体。 正文：句子简短，小标题适当，颜色合适。 举例：

GGL, a GASA-like gene, may play roles in GA-mediated corolla cell growthduring Gerbera hybrida inflorescence developmentJun-ong Pene/063IntroductioreasatmhrnppaEapeIsteracecflower type:nPaneprliesortant role bochASCOTFig2 1GASA family (A) and GGL (B)3.GGL is transeribed in young ray florets andMoraofthulatedGA, They mayplay roles in delongating cellsIVregulated by GAGLNorthern blot hybridization analysis revealed that GGLegulation on its expressionResults:1, Cloning of GGLAGLERNAEIRNA--Reverse transeripti3CImedium, very owevel ofGGLgrowninwitroorPCR with one pairsef dearncrate primerng ofcorollagroutaand high level of GGLexpression3-RACEand S-RACE2248721228726mplelccGLaRNA42. Sequence analysisrDCnftein of 150amits.CeGGFutureworrmine the funetion of GGL usinsRefeMann.ECYPLCVVRKE4X.WGGRPNC1HS教学手段与方法福讲授的主要思路：通过举例，让学生了解并初步了解如何进行学术交流。思考题、讨论题、作业

GGL, a GASA-like gene, may play roles in GA-mediated corolla cell growth during Gerbera hybrida inflorescence development Jian-Zong Peng , Xiao-Jing Wang College of Life Sciences, South China Normal University, Guangdong Key Lab of Biotechnology for Plant Development, Guangzhou 510631 Results: 1. Cloning of GGL Total RNA was extracted from ray florets corolla at early stage of developmemt Reverse transcription PCR with one pairs of degenerate primers 3-RACE and 5-RACE complete cDNA Analysis of the cDNA sequences indicated that GGL encodes a protein of 150 amino acids. GGL has three distinct domains: a putative signal peptide of 21 residues, a cysteine-rich C-terminal domain and a proline-rich repeats domain of 69 residues between signal peptide and cysteine rich domain. The proline-rich repeats domain, which contains the pentapeptide motif PPVXK, is characteristic of other PRPs. It is likely that GGL is a novel small cell wall protein involving in plant cell growth. 2. Sequence analysis Gerbera, as a typical member of Asteraceae, bears three flower types in a single inflorescence: the ray florets (Rf), disc florets (Df) and the trans florets (Tf). The growth and pigmentation of Rf would determine the ornamental character of Gerbera. We have found that light and GA play an important role both in anthocyanin accumulation and flower growth of ray florets. The GASA gene family is a class of genes previously identified in tomato, petunia, Arabidopsis. Most of them are up-regulated by GA. They may play roles in dividing or elongating cells In the present work, we isolated a GASA-like gene (GGL) from ray florets of Gerbera hybrida and analyzed GA regulation on its expression. MKSHLFLLASFLLATTCAAYVAKPPVPAKTAPPPPV LKAPPPPVAKPPTTSPPVAKPPTTAPPVAKPPTTAPP VVKPPPATPPTPPRNTKECYPLCVVRCKLHSRQNVC LRACVTCCDRCKCVPPGHYGNKEVCKCWANMKTH GGRPKCP putative signal peptide proline-rich repeats domain (PRP-like domain) conserved cysteine-rich domain (GASA-like domain) 20AA 70AA 60AA NH2- -COOH putative signal peptide variable hydrophilic domain conserved cysteine-rich domain 18-29AA 7-31 AA 60AA NH2- -COOH Fig 1 sequence of the deduced product of GGL Fig 2 Domain comparison between GASA family (A) and GGL (B) A B 3.GGL is transcribed in young ray florets and regulated by GA Northern blot hybridization analysis revealed that GGL expression was high in early growth of corolla in ray florets and it was not detectable when corolla fully opened. ray florets at different development stage GGL mRNA rRNA Fig 3 GGL expression during corolla development When detached inflorescences at very young stage were grown in vitro on sucrose medium, very low level of GGL expression coincided with the ceasing of corolla growth. GA3 application in the medium was necessary to maintain corolla elongation and high level of GGL expression. -GA3 +GA3 12 24 48 72 96 12 24 48 72 96 hr GGL mRNA rRNA Fig 4 Effect of GA on GGL expression in ray florets during detached inflorescences growing in vitro on sucrose medium Future work We are trying to determine the function of GGL using immuno-localization and transgenic techniques. References Aubert D, Chevilard M, Dorne AM (1998). Expression patterns of GASA genes in Arabidopsis thaliana: the GASA4 gene is up-regulated by gibberellins in meristematic regions. Plant Mol Bio 36, 871-883 Ben-Nissan G, Lee J, Borohov A, Weiss D (2004). GIP, a Petunia hybrida GA-induced cysteine-rich protein: a possible role in shoot elongation and transition to flowering. Plant J 37, 229–238 Meng XC, Wang XJ (2004). Regulation of flower development and anthocyanin accumulation in Gerbera hybrida. J Hort Sci & Biotech 79,131-137 Introduction 教学手段与方法： 讲授的主要思路：通过举例，让学生了解并初步了解如何进行学术交流。 思考题、讨论题、作业：

思考题：参考资料（含参考书、文献等）：学校校园网上的电子资源数据库中的中国期刊网、CSA、ISIProceedings，PubMed等.会议论文集等

思考题： 参考资料（含参考书、文献等）：学校校园网上的电子资源数据库中的中国期刊网、CSA、ISI Proceedings，PubMed 等. 会议论文集等