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《仪器分析》课程教学资源(教案讲义)PH3-10缓冲溶液配制方法

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《仪器分析》课程教学资源(教案讲义)PH3-10缓冲溶液配制方法
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PH3-10缓冲溶液的配制方法1.磷酸氢二钠-柠檬酸缓冲液0.1mol/L0.1mol/L0.2mol/LNa,HPO0.2mol/LNa,HPO4柠檬酸pHpH柠檬酸(毫升)(毫升)(毫升)(毫升)2.25.210.720.4010.609.282.41.2418.765.411.158.852.65.62.1817.8211.608.402.816.835.83.1712.097.913.07.374.1115.896.012.636.23.24.9415.0613.226.783.46.45.7014.3013.856.153.66.4413.566.614.555.453.87.1012.906.815.454.557.04.07.7112.2916.473.534.28.2811.727.217.392.617.44.48.8211.1818.171.839.3510.657.618.734.61.277.84.89.8610.1419.150.855.010.309.708.019.450.55NazHPO分子量=14.98,0.2mol/L溶液为28.40克/升。NaHPO42H,0分子量=178.05,0.2ml/L溶液含35.01克/升。C4H,0,H0分子量=210.14,0.1mol/L溶液为21.01克/升。2.柠檬酸-氢氧化钠-盐酸缓冲液钠离子浓度柠檬酸 (克)盐酸 (毫升)氢氧化钠(克)最终体积(升)①pH(mol/L)CHgO-H,0NaOH97%HCI (浓)2.2210100.20841603.183100.202101163.321083106100.204.30.202108345105.3245144100.35685.80.45285186105106.50.3826615612610①使用时可以每升中加入1克克酚,若最后pH值有变化,再用少量50%氢氧化钠溶液或浓盐酸调节,冰箱保存。3.甘氨酸-盐酸缓冲液(0.05mol/L)X毫升0.2mol/L甘氨酸+Y毫升0.2mol/LHCI,再加水稀释至200毫升YxxYpHpH2.05044.03.05011.42.45032.43.2508.22.65024.23.4506.4503.6505.02.816.8甘氨酸分子量=75.07,0.2mol/L甘氨酸溶液含15.01克/升。4.邻苯二甲酸-盐酸缓冲液(0.05mol/L)X毫升0.2mol/L邻苯二甲酸氢钾+0.2mol/LHCl,再加水稀释到20毫升XYxYpH(20°C)pH(20°C)2.254.0703.251.47052.43.96053.40.99052.63.29553.60.597:52.82.64253.80.26353.02.022

PH3-10缓冲溶液的配制方法 1.磷酸氢二钠–柠檬酸缓冲液 pH 0.2mol/L Na2HPO4 (毫升) 0.1mol/L 柠檬酸 (毫升) pH 0.2mol/L Na2HPO4 (毫升) 0.1mol/L 柠檬酸 (毫升) 2.2 2.4 2.6 2.8 3.0 3.2 3.4 3.6 3.8 4.0 4.2 4.4 4.6 4.8 5.0 0.40 1.24 2.18 3.17 4.11 4.94 5.70 6.44 7.10 7.71 8.28 8.82 9.35 9.86 10.30 10.60 18.76 17.82 16.83 15.89 15.06 14.30 13.56 12.90 12.29 11.72 11.18 10.65 10.14 9.70 5.2 5.4 5.6 5.8 6.0 6.2 6.4 6.6 6.8 7.0 7.2 7.4 7.6 7.8 8.0 10.72 11.15 11.60 12.09 12.63 13.22 13.85 14.55 15.45 16.47 17.39 18.17 18.73 19.15 19.45 9.28 8.85 8.40 7.91 7.37 6.78 6.15 5.45 4.55 3.53 2.61 1.83 1.27 0.85 0.55 Na2HPO4分子量 = 14.98,0.2 mol/L溶液为28.40克/升。 Na2HPO4 ·2H2O分子量 = 178.05,0.2 mol/L溶液含35.01克/升。 C4H2O7 ·H2O分子量 = 210.14,0.1 mol/L溶液为21.01克/升。 2.柠檬酸–氢氧化钠-盐酸缓冲液 pH 钠离子浓度 (mol/L) 柠檬酸(克) C6H8O7 ·H2O 氢氧化钠(克) NaOH 97% 盐酸(毫升) HCl(浓) 最终体积(升)① 2.2 3.1 3.3 4.3 5.3 5.8 6.5 0.20 0.20 0.20 0.20 0.35 0.45 0.38 210 210 210 210 245 285 266 84 83 83 83 144 186 156 160 116 106 45 68 105 126 10 10 10 10 10 10 10 ① 使用时可以每升中加入1克克酚,若最后pH值有变化,再用少量50% 氢氧化钠溶液或浓盐酸调节,冰箱保存。 3. 甘氨酸–盐酸缓冲液(0.05mol/L) X毫升0.2 mol/L甘氨酸+Y毫升0.2 mol/L HCI,再加水稀释至200毫升 pH X Y pH X Y 2.0 2.4 2.6 2.8 50 50 50 50 44.0 32.4 24.2 16.8 3.0 3.2 3.4 3.6 50 50 50 50 11.4 8.2 6.4 5.0 甘氨酸分子量 = 75.07,0.2 mol/L甘氨酸溶液含15.01克/升。 4.邻苯二甲酸–盐酸缓冲液(0.05 mol/L) X毫升0.2 mol/L邻苯二甲酸氢钾 + 0.2 mol/L HCl,再加水稀释到20毫升 pH(20℃) X Y pH(20℃) X Y 2.2 2.4 2.6 2.8 3.0 5 5 5 5 5 4.070 3.960 3.295 2.642 2.022 3.2 3.4 3.6 3.8 5 5 5 5 1.470 0.990 0.597 0.263

邻苯二甲酸氢钾分子量=204.23,0.2m0l/L邻苯二甲酸氢溶液含40.85克/升柠檬酸-柠檬酸钠缓冲液(0.1mol/L)5.0.1 mol/L0.1mol/L0.1mol/L0.1 mol/LPH柠檬酸柠檬酸钠pH柠檬酸柠檬酸钠(毫升)(毫升)(毫升)(毫升)1.43.018.65.08.211.83.217.22.85.27.312.73.44.016.05.46.413.65.13.614.95.65.514.53.86.014.05.84.715.34.013.16.96.03.816.24.212.37.76.22.817.24.48.611.46.42.018.010.39.74.66.61.418.69.24.810.8柠檬酸CgH.0H,0:分子量210.14,0.1mol/L溶液为21.01克/升。柠檬酸钠NasCgH,Oz2HO:分子量294.12,0.1mol/L溶液为29.41克/升。柠檬酸CgHg07H20:2.1g柠檬酸钠Na3CgH,072H20:2.94gH20:100ml乙酸-乙酸钠缓冲液(0.2mol/L)6.0.2 mol/L0.3 mol/L0.2mol/L0.3mol/LHAcHAcNaAcNaAcpH(18℃C)pH(18°℃)(毫升)(毫升)(毫升)(毫升)2.60.759.254.85.904.103.81.208.805.07.003.008.205.24.01.807.902.104.27.355.42.658.601.404.45.63.706.309.100.904.64.905.105.89.400.60NazAc.3H20分子量=136.09,0.2mol/L溶液为27.22克/升。7.磷酸盐缓冲液(1)磷酸氢二钠-磷酸二氢钠缓冲液(0.2)0.2 mol/L0.3 mol/L0.2 mol/L0.3mol/LNa,HPO4NaH,PO4Na,HPO4NaH,PO4PHpH(毫升)(毫升)(毫升)(毫升)5.88.092.07.061.039.05.910.090.07.167.033.012.387.76.07.272.028.06.115.085.07.377.023.06.218.581.57.481.019.077.56.322.57.584.016.06.426.573.57.687.013.06.531.568.57.789.510.56.637.562.57.891.58.56.743.556.57.993.07.06.849.551.08.094.75.36.945.055.0NazHPO4-2H2O分子量=178.05,0.2mol/L溶液为85.61克/升

邻苯二甲酸氢钾分子量 = 204.23,0.2 mol/L邻苯二甲酸氢溶液含40.85克/升 5.柠檬酸–柠檬酸钠缓冲液(0.1 mol/L) pH 0.1 mol/L 柠檬酸 (毫升) 0.1 mol/L 柠檬酸钠 (毫升) pH 0.1 mol/L 柠檬酸 (毫升) 0.1 mol/L 柠檬酸钠 (毫升) 3.0 3.2 3.4 3.6 3.8 4.0 4.2 4.4 4.6 4.8 18.6 17.2 16.0 14.9 14.0 13.1 12.3 11.4 10.3 9.2 1.4 2.8 4.0 5.1 6.0 6.9 7.7 8.6 9.7 10.8 5.0 5.2 5.4 5.6 5.8 6.0 6.2 6.4 6.6 8.2 7.3 6.4 5.5 4.7 3.8 2.8 2.0 1.4 11.8 12.7 13.6 14.5 15.3 16.2 17.2 18.0 18.6 柠檬酸C6H8O7 ·H2O:分子量210.14,0.1 mol/L溶液为21.01克/升。 柠檬酸钠Na3 C6H5O7 ·2H2O:分子量294.12,0.1 mol/L溶液为29.41克/升。 柠檬酸C6H8O7 ·H2O: 2.1g 柠檬酸钠Na3 C6H5O7 ·2H2O: 2.94g H2O: 100 ml 6.乙酸–乙酸钠缓冲液(0.2 mol/L) pH(18℃) 0.2 mol/L NaAc (毫升) 0.3 mol/L HAc (毫升) pH(18℃) 0.2 mol/L NaAc (毫升) 0.3 mol/L HAc (毫升) 2.6 3.8 4.0 4.2 4.4 4.6 0.75 1.20 1.80 2.65 3.70 4.90 9.25 8.80 8.20 7.35 6.30 5.10 4.8 5.0 5.2 5.4 5.6 5.8 5.90 7.00 7.90 8.60 9.10 9.40 4.10 3.00 2.10 1.40 0.90 0.60 Na2Ac·3H2O分子量 = 136.09,0.2 mol/L溶液为27.22克/升。 7.磷酸盐缓冲液 (1)磷酸氢二钠–磷酸二氢钠缓冲液(0.2) pH 0.2 mol/L Na2HPO4 (毫升) 0.3 mol/L NaH2PO4 (毫升) pH 0.2 mol/L Na2HPO4 (毫升) 0.3 mol/L NaH2PO4 (毫升) 5.8 5.9 6.0 6.1 6.2 6.3 6.4 6.5 6.6 6.7 6.8 6.9 8.0 10.0 12.3 15.0 18.5 22.5 26.5 31.5 37.5 43.5 49.5 55.0 92.0 90.0 87.7 85.0 81.5 77.5 73.5 68.5 62.5 56.5 51.0 45.0 7.0 7.1 7.2 7.3 7.4 7.5 7.6 7.7 7.8 7.9 8.0 61.0 67.0 72.0 77.0 81.0 84.0 87.0 89.5 91.5 93.0 94.7 39.0 33.0 28.0 23.0 19.0 16.0 13.0 10.5 8.5 7.0 5.3 Na2HPO4 ·2H2O分子量 = 178.05,0.2 mol/L溶液为85.61克/升

NaHPO?12HO分子量=358.22,0.2mol/L溶液为71.64克/升NaHPO42H,0分子量=156.03,0.2mol/L溶液为31.21克/升。(2)磷酸氢二钠-磷酸二氢钾缓冲液(1/15mol/L)mol/L/15Na2HPO4mol/L/15KH2PO4mol/L/15Na2HPO4mol/L//15KH2PO4pHpH(毫升)(毫升)(毫升)(毫升)4.920.109.907.177.003.005.290.509.507.388.002.005.911.009.007.739.001.006.242.008.008.049.500.503.006.477.008.349.750.256.644.006.008.679.900.106.815.005.008.1810.0006.986.004.00NaHPO2H,0分子量=178.051/15M溶液为11.876克/升。KH,PO4分子量=136.09,1/15M溶液为9.078克/升。8.磷酸二氢钾-氢氧化钠缓冲液(0.05M)X毫升0.2MK,PO+Y毫升0.2NNaOH加水稀释至29毫升pH(20°C)pH(20℃)X(毫升)Y (毫升)X(毫升)Y (毫升)555.80.3727.02.963557.26.00.5703.500556.20.8607.43.950556.41.2607.64.28057.856.61.7804.520556.88.02.3654.680巴比妥钠-盐酸缓冲液(18℃)9.0.04M巴比妥钠溶0.04M巴比妥钠pHpH0.2V盐酸0.2N盐酸(毫升)液溶液(毫升)6.810010018.48.45.217.010017.81003.828.67.210016.71002.528.87.410015.31009.01.657.610013.41009.21.137.810011.471009.40.701009.398.01009.60.358.21007.21100巴比妥钠盐分子量=206.18;0.04M溶液为8.25克/升10.Tris-盐酸缓冲液(0.05M,25C)50毫升0.1M三羟甲基氨基甲烷(Tris)溶液与X毫升0.1N盐酸混匀后,加水稀释至100毫升pHpHX(毫升)X(毫升)7.1045.78.1026.27.2044.78.2022.98.307.3043.419.97.408.4017.242.07.5040.38.5014.77.6038.58.6012.47.7036.68.7010.37.8034.58.808.57.908.907.0

Na2HPO4 ·12H2O分子量 = 358.22,0.2 mol/L溶液为71.64克/升。 Na2HPO4 ·2H2O分子量 = 156.03,0.2 mol/L溶液为31.21克/升。 (2)磷酸氢二钠–磷酸二氢钾缓冲液(1/15 mol/L) pH mol/L/15Na2HPO4 (毫升) mol/L//15KH2PO4 (毫升) pH mol/L//15Na2HPO4 (毫升) mol/L//15KH2PO4 (毫升) 4.92 5.29 5.91 6.24 6.47 6.64 6.81 6.98 0.10 0.50 1.00 2.00 3.00 4.00 5.00 6.00 9.90 9.50 9.00 8.00 7.00 6.00 5.00 4.00 7.17 7.38 7.73 8.04 8.34 8.67 8.18 7.00 8.00 9.00 9.50 9.75 9.90 10.00 3.00 2.00 1.00 0.50 0.25 0.10 0 Na2HPO4 ·2H2O分子量 = 178.05,1/15M溶液为11.876克/升。 KH2PO4分子量 = 136.09,1/15M溶液为9.078克/升。 8.磷酸二氢钾–氢氧化钠缓冲液(0.05M) X毫升0.2M K2PO4 + Y毫升0.2N NaOH加水稀释至29毫升 pH(20℃) X (毫升) Y(毫升) pH(20℃) X (毫升) Y(毫升) 5.8 6.0 6.2 6.4 6.6 6.8 5 5 5 5 5 5 0.372 0.570 0.860 1.260 1.780 2.365 7.0 7.2 7.4 7.6 7.8 8.0 5 5 5 5 5 5 2.963 3.500 3.950 4.280 4.520 4.680 9.巴比妥钠-盐酸缓冲液(18℃) pH 0.04M巴比妥钠溶 液 0.2V盐酸 pH 0.04M巴比妥钠 溶液(毫升) 0.2N盐酸(毫升) 6.8 7.0 7.2 7.4 7.6 7.8 8.0 8.2 100 100 100 100 100 100 100 100 18.4 17.8 16.7 15.3 13.4 11.47 9.39 7.21 8.4 8.6 8.8 9.0 9.2 9.4 9.6 100 100 100 100 100 100 100 100 5.21 3.82 2.52 1.65 1.13 0.70 0.35 巴比妥钠盐分子量=206.18;0.04M溶液为8.25克/升 10.Tris–盐酸缓冲液(0.05M,25℃) 50毫升0.1M三羟甲基氨基甲烷(Tris)溶液与X毫升0.1N盐酸混匀后,加水稀释至100毫升。 pH X(毫升) pH X(毫升) 7.10 7.20 7.30 7.40 7.50 7.60 7.70 7.80 7.90 45.7 44.7 43.4 42.0 40.3 38.5 36.6 34.5 8.10 8.20 8.30 8.40 8.50 8.60 8.70 8.80 8.90 26.2 22.9 19.9 17.2 14.7 12.4 10.3 8.5 7.0

32.08.0029.2三羟甲基氨基甲烷(Tris)HOCH2CH2OHcHOCH2 NH2分子量=121.14;0.1M溶液为12.114克/升。Tris溶液可从空气中吸收二氧化碳,使用时注意将瓶盖严硼酸-硼砂缓冲液(0.2M硼酸根)11.0.05M硼砂(毫0.2M硼砂(毫0.05M硼砂(毫0.2M硼酸(毫pHpH升)升)升)升)7.41.09.08.23.56.58.57.61.58.44.55.57.82.08.08.76.04.08.03.07.09.08.02.0硼砂NazB4O7H,0,分子量=381.430.05M溶液(=0.2M硼酸根)含19.07克/升。硼酸H,BO,分子量=61.84.0.2M溶液为12.37克/升。硼砂易失去结晶水,必须在带塞的瓶中保存。11.硼酸-硼砂缓冲液(0.2M硼酸根)0.05M硼砂(毫0.2M硼酸(毫0.05M硼砂(毫0.2M硼酸(毫pHpH升)升)升)升)7.41.09.08.23.56.51.58.55.57.68.44.57.82.08.08.76.04.08.03.07.09.08.02.0硼砂NazB4O710H,0,分子量=381.43;0.05M溶液(=0.2M硼酸根)含19.07克/升。硼酸H,BO3,分子量=61.84,0.2M溶液为12.37克/升。硼砂易失去结晶水,必须在带塞的瓶中保存。12.甘氨酸-氢氧化钠缓冲液(0.05M)X毫升0.2M甘氨酸+Y毫升0.2NnaOH加水稀释至200毫升YpHxxYpH8.6504.09.65022.48.8506.09.85027.29.0508.85032.010.09.25012.010.45038.69.4505045.516.810.6甘氨酸分子量=75.07;0.2M溶液含15.01克/升。13.硼砂-氢氧化钠缓冲液(0.05M硼酸根)X毫升0.05M硼砂+Y毫升0.2NNaOH加水稀释至200毫升xYxYpHpH9.3506.09.85034.09.45011.010.05043.050509.623.010.146.0

8.00 32.0 29.2 三羟甲基氨基甲烷(Tris)HOCH2 CH2OH C HOCH2 NH2 分子量=121.14; 0. 1M溶液为12.114克/升。Tris溶液可从空气中吸收二氧化碳,使用时注意将瓶盖严。 11.硼酸–硼砂缓冲液(0.2M硼酸根) pH 0.05M硼砂(毫 升) 0.2M 硼 砂 ( 毫 升) pH 0.05M硼砂(毫 升) 0.2M 硼 酸 ( 毫 升) 7.4 7.6 7.8 8.0 1.0 1.5 2.0 3.0 9.0 8.5 8.0 7.0 8.2 8.4 8.7 9.0 3.5 4.5 6.0 8.0 6.5 5.5 4.0 2.0 硼砂Na2B4O7 ·H2O,分子量=381.43;0.05M溶液(=0.2M硼酸根)含19.07克/升。 硼酸H2BO3 ,分子量=61.84,0.2M溶液为12.37克/升。 硼砂易失去结晶水,必须在带塞的瓶中保存。 11.硼酸-硼砂缓冲液(0.2M硼酸根) pH 0.05M硼砂(毫 升) 0.2M 硼 酸 ( 毫 升) pH 0.05M硼砂(毫 升) 0.2M 硼 酸 ( 毫 升) 7.4 7.6 7.8 8.0 1.0 1.5 2.0 3.0 9.0 8.5 8.0 7.0 8.2 8.4 8.7 9.0 3.5 4.5 6.0 8.0 6.5 5.5 4.0 2.0 硼砂Na2B4O7 ·10H2O,分子量=381.43;0.05M溶液(=0.2M硼酸根)含19.07克/升。 硼酸H2BO3,分子量=61.84, 0.2M溶液为12.37克/升。 硼砂 易失去结晶水,必须在带塞的瓶中保存。 12.甘氨酸–氢氧化钠缓冲液(0.05M) X毫升0.2M甘氨酸+Y毫升0.2NnaOH加水稀释至200毫升 pH X Y pH X Y 8.6 8.8 9.0 9.2 9.4 50 50 50 50 50 4.0 6.0 8.8 12.0 16.8 9.6 9.8 10.0 10.4 10.6 50 50 50 50 50 22.4 27.2 32.0 38.6 45.5 甘氨酸分子量=75.07;0.2M溶液含15.01克/升。 13.硼砂-氢氧化钠缓冲液(0.05M硼酸根) X毫升0.05M硼砂+Y毫升0.2NNaOH加水稀释至200毫升 pH X Y pH X Y 9.3 9.4 9.6 50 50 50 6.0 11.0 23.0 9.8 10.0 10.1 50 50 50 34.0 43.0 46.0

硼砂Na,B.O-10H,O.分子量=381.43;0.05M溶液为19.07克/升。碳酸钠-碳酸氢钠缓冲液(0.1M)14.Ca2+、Mg2+存在时不得使用pH0.1MNa2CO3(毫升)0.1MN,HCO3(毫升)37℃20℃99.168.771289.409.12739.519.409.789.504659.909.72510.149.90641310.2810.088210.5310.289110.8310.57NazCO,10H0分子量=286.20.1M溶液为28.62克/升。N2HCO;分子量=84.0;0.1M溶液为8.40克/升。15."PBS"缓冲液7.4 PH7.67.27.0H,0100010001000100mlNaCI8.58.58.58.5gNa,HPO42.22.22.22.2g0.10.20.3NaH2PO40.4g

硼砂Na2B4O7 ·10H2O,分子量=381.43;0.05M溶液为19.07克/升。 14.碳酸钠-碳酸氢钠缓冲液(0.1M) Ca 2+、Mg 2+存在时不得使用 pH 0.1MNa2CO3 (毫升) 0.1MN2HCO3 (毫升) 20℃ 37℃ 9.16 9.40 9.51 9.78 9.90 10.14 10.28 10.53 10.83 8.77 9.12 9.40 9.50 9.72 9.90 10.08 10.28 10.57 1 2 3 4 5 6 7 8 9 9 8 7 6 5 4 3 2 1 Na2CO2 ·10H2O分子量=286.2;0.1M溶液为28.62克/升。 N2HCO3分子量=84.0;0.1M溶液为8.40克/升。 15."PBS"缓冲液 PH 7.6 7.4 7.2 7.0 H2O NaCl Na2HPO4 NaH2PO4 1000 8.5 2.2 0.1 1000 8.5 2.2 0.2 1000 8.5 2.2 0.3 100ml 8.5g 2.2g 0.4g

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