南京师范大学：《微生物学》课程PPT教学课件（英文版）Chapter 5 Microbial Nutrition and Culture Medium

Chapter 5 Microbial Nutrition and Culture medium

Chapter 5 Microbial Nutrition and Culture medium

Nutrition Nutrition is a process by which chemical substances called nutrients are required from the environment and used for the cellular activities of metabolism and growth. Nutrient:the substance could be used by microbes for metabolism or reproduction of cells

Nutrition ： Nutrition is a process by which chemical substances called nutrients are required from the environment and used for the cellular activities of metabolism and growth. Nutrient：the substance could be used by microbes for metabolism or reproduction of cells

Nutrient provide the structural substance,energy, metabolic regulate substance and appropriate physiological environment. Some microbes could use nonmaterial energy-light

Nutrient provide the structural substance , energy, metabolic regulate substance and appropriate physiological environment. Some microbes could use nonmaterial energy-light

Section 1 Six kinds of essential nutrient for microbes I.cellular chemical constitutes It's much alike in the life in the element The main elements areC、H、O、N(90%-g7%to total dry cell weight),Cis about 50%,the others are inorganic nutrient.C/N is 5:1 usually

I. cellular chemical constitutes It’s much alike in the life in the element . The main elements are C、H、O、N (90%-97% to total dry cell weight), C is about 50%, the others are inorganic nutrient. C/N is 5:1 usually. Section 1 Six kinds of essential nutrient for microbes

element ☑Macronutrient:C、H、O、N、P、S ☑others element:K、Na、Ca、Mg、Fe、Mn、 Cu、Co、Zn、Mo,etal ■Existence form: ■☑organic:protein、sugar、lipoid、 nucleic acid、vitamin、decomposition substance,metabolic medial products u☑inorganic salt ash ■☑Vater-.=-70%~90%to wet cell weight

element ØMacronutrient ：C、H、O、N、P、 S Øothers element：K、Na、Ca、Mg、Fe、Mn、 Cu、Co、Zn、Mo, et al Existence form: Ø organic：protein、sugar、lipoid、 nucleic acid、vitamin、decomposition substance、metabolic medial products Ø inorganic salt ash Ø Water-70% ~ 90% to wet cell weight

Table 1.The macronutrient content in microbes ( to dry cell weight) element bacteria yeast mould 50 49.8 47.0 15 7.5 5.2 8 5.7 6.7 20 31.1 40.2 3 1.5 1.2 0.3 0.2

element bacteria yeast mould C 50 49.8 47.0 N 15 7.5 5.2 H 8 5.7 6.7 O 20 31.1 40.2 P 3 1.5 1.2 S 1 0.3 0.2 Table 1.The macronutrient content in microbes (% to dry cell weight)

Microbes is omnivorous. The substance utilized by generic species could be utilized by microbes too. The substance couldn't be utilized by generic species could be utilized by microbes. Some kinds of substance harmful to generic species also could be utilized by microbes

The substance utilized by generic species could be utilized by microbes too. The substance couldn’t be utilized by generic species could be utilized by microbes. Some kinds of substance harmful to generic species also could be utilized by microbes. Microbes is omnivorous

ll.Main nutrient and the function ■Carbon source ■Nitrogen source ■Energy source Growth factor ☐Inorganic salt Water

II. Main nutrient and the function Carbon source Nitrogen source Energy source Growth factor Inorganic salt Water

Functions: vconstitute the cells of the microbes uvprovide the energy needed in the physiological activity to microbes uvthe source to form the microbial metabolic products uNutrient is the substance base for microbial metabolism and living activity. If lose this base,the life will die

Functions: vconstitute the cells of the microbes vprovide the energy needed in the physiological activity to microbes vthe source to form the microbial metabolic products Nutrient is the substance base for microbial metabolism and living activity. If lose this base, the life will die

1.Carbon sources The nutrient provide the carbon element for microbies Organic carbon sources:protein,nucleic acid,starch,glucose,et al Inorganic acid:CO2,Na,CO3,CaCO3 et al Heterotrophic microbe:is an organism must obtain its carbon in an organic form. Autotrophic microbe:is an organism that uses CO2,an inorganic gas,as its carbon source

Heterotrophic microbe: is an organism must obtain its carbon in an organic form. Autotrophic microbe: is an organism that uses CO2 , an inorganic gas, as its carbon source. 1.Carbon sources The nutrient provide the carbon element for microbies ❑Organic carbon sources: protein, nucleic acid, starch, glucose, et al ❑Inorganic acid: CO2 , Na2CO3 , CaCO3 et al