《药物分析》课程教学资源（PPT课件）第9章 维生素类药物分析 The Analysis of Vitamins

The Analysis of Vitamins 维生素类药物分析

The Analysis of Vitamins 维生素类药物分析

掌握维生素B1、维生素C、及其制剂的 鉴别、检查和含量测定方法 熟悉维生素A的鉴别和含量测定方法 了解维生素D、E的鉴别和含量测定方法

 掌握维生素B1、维生素C、及其制剂的 鉴别、检查和含量测定方法  熟悉维生素A的鉴别和含量测定方法  了解维生素D、E的鉴别和含量测定方法

CHARACTERS 性质 Retinol acetate occurs as pale-yellow crystals(melting point about 60C). Once melted retinol acetate tends to yield a supercooled melt. Retinol propionate occurs as a reddish-brown oily liquid. Retinol palmitate is a fat-like,light yellow solid or a yellow oily liquid,if melted (melting point about 26C). All retinol esters are practically insoluble in water,soluble or partly soluble in ethanol and miscible with organic solvents.Vitamin A and its esters are very sensitive to the action of air,oxidising agents,acids,light and heat. Carry out the assay and all tests as rapidly as possible,avoiding exposure to actinic light and air,oxidising agents,oxidation catalysts(e.g.copper,iron), acids and heat;use freshly prepared solutions

 CHARACTERS 性质 Retinol acetate occurs as pale-yellow crystals (melting point about 60 ﾟC). Once melted retinol acetate tends to yield a supercooled melt. Retinol propionate occurs as a reddish-brown oily liquid. Retinol palmitate is a fat-like, light yellow solid or a yellow oily liquid, if melted (melting point about 26ﾟC). All retinol esters are practically insoluble in water, soluble or partly soluble in ethanol and miscible with organic solvents. Vitamin A and its esters are very sensitive to the action of air, oxidising agents, acids, light and heat. Carry out the assay and all tests as rapidly as possible, avoiding exposure to actinic light and air, oxidising agents, oxidation catalysts (e.g. copper, iron), acids and heat; use freshly prepared solutions

H3C CH3 CH3 CH3 CH3 R=H C20H300 286.5 R=CO-CH3 C22H3202 328.5 R=CO-C2H5 C23H3402 342.5 R=C0-C15H31C36H60O2 524.9

R = H C20H30O 286.5 R = CO-CH3 C22H32O2 328.5 R = CO-C2H5 C23H34O2 342.5 R = CO-C15H31 C36H60O2 524.9

ASSAY UV三点校正法 A325,corr=6.815A325-2.555A310-4.260A334

ASSAY UV 三点校正法

Impurities A.R H,CO-CHa:kitols (Diels-Alder dimers of vitamin A), H3C CH3 CH3 CH3 CH2 CH3

Impurities A.R = H, CO-CH3: kitols (Diels-Alder dimers of vitamin A)

B,(3E,5E,7)-3,7-dimethyl-9-[(1Z)-2,6,6- trimethylcyclohex-2-enylidene)nona-1,3,5,7- tetraene (anhydro-vitamin A), H3C CH3 CH3 CH3 OH CH3

B,(3E,5E,7E)-3,7-dimethyl-9-[(1Z)-2,6,6- trimethylcyclohex-2-enylidene)nona-1,3,5,7- tetraene (anhydro-vitamin A)

C.(3E,5E,7E)-3,7-dimethyl-9-[(1Z-2,6,6- trimethylcyclohex-2-enylidene)nona-3,5,7- trien-1-ol (retro-vitamin A), D Oxidation products of vitamin A

C. (3E,5E,7E)-3,7-dimethyl-9-[(1Z)-2,6,6- trimethylcyclohex-2-enylidene)nona-3,5,7- trien-1-ol (retro-vitamin A), D. Oxidation products of vitamin A

1 IU of vitamin A 0.300 mg of all-(E)-retinol 0.344 mg of all-(E)-retinol acetate, 0.359 mg of all-(E)-retinol propionate, 0.550 mg of all-(E)-retinol palmitate, 1 mg of retinol equivalent is equivalent to 3333 IU

1 IU of vitamin A 0.300 mg of all-(E)-retinol 0.344 mg of all-(E)-retinol acetate, 0.359 mg of all-(E)-retinol propionate, 0.550 mg of all-(E)-retinol palmitate, 1 mg of retinol equivalent is equivalent to 3333 IU

Impurities R R H H3C CH3 CH3 H3C CH3 H CH3 CH3 H3C CH3 H3C

Impurities