《病原生物学（医学微生物学）》课程教学课件（英文）18_Virio_Vampylo_Helico

Section III BacteriologyVibrios, Campylobacter, HelicobacterSHIHEZI UNIVERSITY

Section III Bacteriology Vibrios, Campylobacter, Helicobacter SHIHEZI UNIVERSITY

KEY TERMS Vibrio choleraeCholeragen Vero toxin (Shiga-like(cholera toxin)Campylobacter HemolysinJejunipiliHelicobacter pyloriYersinia entercoliticaSHIHEZI UNIVERSITY

KEY TERMS  Vibrio cholerae  Vero toxin (Shiga-like) Choleragen (cholera toxin)  Hemolysin Campylobacter jejuni  pili Helicobacter pylori  Yersinia entercolitica SHIHEZI UNIVERSITY

Bacteria InfectedGastrointestinal TractEnterobacteriaceaeescherichia. shigella. salmonella enterobacterklebsiella, serratia, proteus, and others Vibrio CampylobacterHelicobacterSHIHEZIUNIVERSITY

Bacteria Infected Gastrointestinal Tract  Enterobacteriaceae escherichia, shigella, salmonella, enterobacter, klebsiella, serratia, proteus, and others  Vibrio  Campylobacter  Helicobacter SHIHEZI UNIVERSITY

VibiosVibio choleraKINGCHOLERAVibio parahaemolyticusSHIHEZI UNIVERSITY

Vibios Vibio cholera Vibio parahaemolyticus SHIHEZI UNIVERSITY

The Vibrios Vibrios are among the most common bacteria insurface waters worldwide They are curved aerobic rods and are motilepossessing a polar flagellumV cholerae serogroups O1 and 0139 causecholera in humans+while other vibrios may cause sepsis or enteritisSHIHEZIUNIVERSITY

The Vibrios  Vibrios are among the most common bacteria in surface waters worldwide  They are curved aerobic rods and are motile, possessing a polar flagellum  V cholerae serogroups O1 and O139 cause cholera in humans  while other vibrios may cause sepsis or enteritis SHIHEZI UNIVERSITY

VibriosGram negative rods Comma shapedFacultative anaerobes Oxidase positive Simple nutritional requirements Readily cultivateSHIHEZI UNIVERSITY

Vibrios  Gram negative rods  Comma shaped  Facultative anaerobes  Oxidase positive  Simple nutritional requirements  Readily cultivate SHIHEZI UNIVERSITY

CultureThiosulfate-citrate-bile-sucrose (TCBSagar1 oxidase-positivegrow at a very high pH (8.5-9.5)+ rapidly killed by acid.SHIHEZI UNIVERSITY

Culture  Thiosulfate-citrate-bile-sucrose (TCBS) agar  oxidase-positive  grow at a very high pH (8.5–9.5) rapidly killed by acid. SHIHEZI UNIVERSITY

Antigenic Structure & BiologicClassification V cholerae has OTable 18-1. The medically important vibrioslipopolysaccharidesthat confer serologicOrganismHuman DiseasespecificityVcholerae serogroups 01Epidemic and pandemicThere are at least 139and 0139choleraO antigen groupsVcholerae serogroupsCholera-ike diarhea mildV cholerae strains ofnon-01/non-0139diarrhea; rarely, extraintestinalO group 1 and Oinfectiongroup 139 causeclassic choleraV parahaemolyticusGastrentrtis perhapsextraintestinalinfection occasionally, non-01/non- 0i39 V Otherscholerae causesV mimicus,Vvulnifcus,Eawoundofttissueancholera-like diseaseVhliae fuialis,other extraintestinalV damsela, V anginolyinfections,all uncommonticus,VmetschnikoviSHIHEZIUNIVERSITY

Antigenic Structure & Biologic Classification  V cholerae has O lipopolysaccharides that confer serologic specificity  There are at least 139 O antigen groups  V cholerae strains of O group 1 and O group 139 cause classic cholera  occasionally, non- O1/non- O139 V cholerae causes cholera-like disease SHIHEZI UNIVERSITY

Serotypes & BiotypesV cholerae serogroup O1 antigen has determinantsthat make further typing:The main serotypes are Ogawa and InabaTwo biotypes of epidemic V cholerae have been definedclassic and EI TorThe EITor biotype producesahemolysin gives positive resultson the Voges-Proskauer test, and is resistant to polymyxinBMolecular techniques can also be used to type V choleraeTyping is used for epidemiologic studies, and testsgenerally are done only in reference laboratoriesSHIHEZI UNIVERSITY

Serotypes & Biotypes  V cholerae serogroup O1 antigen has determinants that make further typing:  The main serotypes are Ogawa and Inaba  Two biotypes of epidemic V cholerae have been defined, classic and El Tor  The El Tor biotype produces a hemolysin, gives positive results on the Voges-Proskauer test, and is resistant to polymyxin B. Molecular techniques can also be used to type V cholerae  Typing is used for epidemiologic studies, and tests generally are done only in reference laboratories SHIHEZI UNIVERSITY

Occurrence - choleraCholera,2002-2003Third world?Common+ Food hygiene* Ingestion ofsea-food Other locations* Travelers,uncommonCountrieslareasreporingcholeracaseCountrieswithimportedcholeracasesSHIHEZI UNIVERSITY

Occurrence - cholera  Third world  Common * Food hygiene * Ingestion of sea-food * Other locations * Travelers, uncommon SHIHEZI UNIVERSITY