《病原生物学（医学微生物学）》课程教学课件（英文）12_Nesseria

Section II BacteriologyThe NeisseriaeSHIHEZI UNIVERSITY

Section III Bacteriology The Neisseriae SHIHEZI UNIVERSITY

KEY TERMS NeisseriaNeisseriae Diplococci Thayer Martin chocolate agar N. gonorrhoeae GonorrheaSexually transmitted disease, STDN. meningitidlisMeningitisSHIHEZI UNIVERSITY

KEY TERMS  Neisseria Neisseriae  Diplococci  Thayer Martin chocolate agar  N. gonorrhoeae  Gonorrhea  Sexually transmitted disease, STD  N. meningitidis  Meningitis SHIHEZI UNIVERSITY

NeisseriaGram negative, diplococcusN. meningitidisN. gonorrhoeaeN. siccaN. subflavaN. flavescensN. mucosaSHIHEZI UNIVERSITY

Neisseria  Gram negative, diplococcus N. meningitidis N. gonorrhoeae N. sicca N. subflava N. flavescens N. mucosa SHIHEZI UNIVERSITY

Neisseria gonorrheaGonococcusSHIHEZI UNIVERSITY

Neisseria gonorrhea Gonococcus SHIHEZI UNIVERSITY

Morphology0.8 μ m, Gram negative, diplococci (pairs of cocci most in neutrophil cellsSHIHEZIUNIVERSITY

Morphology  0.8μm, Gram negative, diplococci (pairs of cocci)  most in neutrophil cells SHIHEZI UNIVERSITY

Figure21-1.Gramstainofaurethralexudateofapatientwithgonorhea.Nucleiandcellmembraneoutinesofmanypolymorphonuclearcellsareseen(twshownbylargearows).lntracellulargram-negativediplococci (Neisseria gonorrhoeae) in clumps aremarked by thesmall arrows.SHIHEZIUNIVERSITY

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Neisseragonorrhoeae-coccoidprokarye divdingcausesgonha(SEMx40000)DrDennisKunkel,UniversityofHawaiUsed with permissionSHIHEZIUNIVERSITY

Neisseria gonorrhoeae - coccoid prokaryote (dividing); causes gonorrhea (SEM x 40,000) © Dr Dennis Kunkel, University of Hawaii. Used with permission SHIHEZI UNIVERSITY

CultivationCultureObligatory aerobicThese bacteria grow best on chocolate agar so-calledbecause it contains heated blood brown in color Thayer Martin Chocolate Agar A modified (selective) chocolate agar Biological reactions+Glucose fermentation onlyCan not use other sugarsSHIHEZI UNIVERSITY

Cultivation  Culture  Obligatory aerobic  These bacteria grow best on chocolate agar so-called because it contains heated blood brown in color  Thayer Martin Chocolate Agar  A modified (selective) chocolate agar  Biological reactions  Glucose fermentation only  Can not use other sugars SHIHEZI UNIVERSITY

Oxidase Test The colonies areoxidase positivewhich isdemonstrated byflooding the platewith a dye which onoxidation changescolorPhoto by Karen M.KiscrSHIHEZI UNIVERSITY

Oxidase Test  The colonies are oxidase positive which is demonstrated by flooding the plate with a dye which on oxidation changes color SHIHEZI UNIVERSITY

Surface Antigenic StructuresOutermembranePeptidoglycanCell envelopeCytoplasmicPeptidoglycanmembraneOutermembraneCytoplasmicmembranePilusPilusFigure21-2.CollageanddrawingofNgonorrhoeaeshowing piliand the threelayers ofthe cellenvelopeSHIHEZIUNIVERSITY

Surface Antigenic Structures SHIHEZI UNIVERSITY