复旦大学：《医学微生物学 Medical Microbiology（MBBS）》课程讲义_理论课_Lecture21-Hepatitis A, C, E

Hepatitis a, C, E Zhigang Yi(易志刚) MOH&MOE Key Lab of Medical Molecular Virology Shanghai Medical College, Fudan University

Hepa%%s'A,'C,'E Zhigang'Yi'（易志刚） MOH&MOE Key Lab of Medical Molecular Virology Shanghai Medical College, Fudan University

Characteristics of Hepatitis viruses Viru Hepatitis a Hepatitis c Hepatitis e Family Picornaviridae Flaviviridae Hepeviridae Envelope Stability Heat and acid-stable Ether-sensitive Heat-stable acid-sensitive Genome + SsRNA SsrnA + SSRNa Genome size 7.5kb 9.6kb 7.2kb Transmission Fecal-oral Parenteral Chronic disease Nevel Vaccine Y∈ No Yes Oncogenic Yes No

Characteris%cs'of'Hepa%%s'Viruses' Family'''''''''''''''''Picornaviridae''''''''''''''''''''''Flaviviridae'''''''''''''''''''''''''''Hepeviridae'''''''''''''''''''''' Virus''''''''''''''''''''''Hepa%%s'A'''''''''''''''''''''''Hepa%%s'C'''''''''''''''''''''''''''''''''Hepa%%s'E' Envelope''''''''''''''''No'''''''''''''''''''''''''''''''''''''''''Yes''''''''''''''''''''''''''''''''''''''''''''No' Genome'''''''''''''''+'ssRNA''''''''''''''''''''''''''''''''+'ssRNA'''''''''''''''''''''''''''''''''''+'ssRNA''''''''''''''''''''' Genome'size'''''''''''7.5kb''''''''''''''''''''''''''''''''''9.6kb''''''''''''''''''''''''''''''''''''''''''7.2kb''''''''''''''''''''''''''''''''''' Stability''''''''''''''''Heat'and'acidNstable''''''''''EtherNsensi%ve,'''''''''''''''''''HeatNstable''' '''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''acidNsensi%ve''''''''' Transmission'''''''''''FecalNoral'''''''''''''''''''''Parenteral'''''''''''''''''''''''''''''''''FecalNoral' Chronic'disease'''''''''Never''''''''''''''''''''''''''''Yes'''''''''''''''''''''''''''''''''''''''''''''''Never' Vaccine''''''''''''''''''''''''Yes''''''''''''''''''''''''''''''''''No''''''''''''''''''''''''''''''''''''''''''''''''Yes' Oncogenic''''''''''''''''''No''''''''''''''''''''''''''''''''''Yes''''''''''''''''''''''''''''''''''''''''''''''''No

Characteristics of Hepatitis viruses Viru Hepatitis a Hepatitis c Hepatitis e Family Picornaviridae Flaviviridae Hepeviridae Envelope Stability Heat and acid-stable Ether-sensitive Heat-stable acid-sensitive Genome + Ssrna + ssRNA ssRNA Genome size 7.5kb 9.6kb 7.2kb Transmission Fecal-oral Parenteral Chronic disease Nevel Vaccine Y∈ No Yes Oncogenic Yes No

Characteris%cs'of'Hepa%%s'Viruses' Family'''''''''''''''''Picornaviridae''''''''''''''''''''''Flaviviridae'''''''''''''''''''''''''''Hepeviridae'''''''''''''''''''''' Virus''''''''''''''''''''''Hepa%%s'A'''''''''''''''''''''''Hepa%%s'C'''''''''''''''''''''''''''''''''Hepa%%s'E' Envelope''''''''''''''''No'''''''''''''''''''''''''''''''''''''''''Yes''''''''''''''''''''''''''''''''''''''''''''No' Genome'''''''''''''''+'ssRNA''''''''''''''''''''''''''''''''+'ssRNA'''''''''''''''''''''''''''''''''''+'ssRNA''''''''''''''''''''' Genome'size'''''''''''7.5kb''''''''''''''''''''''''''''''''''9.6kb''''''''''''''''''''''''''''''''''''''''''7.2kb''''''''''''''''''''''''''''''''''' Stability''''''''''''''''Heat'and'acidNstable''''''''''EtherNsensi%ve,'''''''''''''''''''HeatNstable''' '''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''acidNsensi%ve''''''''' Transmission'''''''''''FecalNoral'''''''''''''''''''''Parenteral'''''''''''''''''''''''''''''''''FecalNoral' Chronic'disease'''''''''Never''''''''''''''''''''''''''''Yes'''''''''''''''''''''''''''''''''''''''''''''''Never' Vaccine''''''''''''''''''''''''Yes''''''''''''''''''''''''''''''''''No''''''''''''''''''''''''''''''''''''''''''''''''Yes' Oncogenic''''''''''''''''''No''''''''''''''''''''''''''''''''''Yes''''''''''''''''''''''''''''''''''''''''''''''''No

Characteristics of Hepatitis viruses Viru Hepatitis A Hepatitis c Hepatitis e Family Picornaviridae Flaviviridae Hepeviridae Envelope Stability Heat and acid-stableEther-sensitive Heat-stable acid-sensitive Genome + SSRNA + ssrna ssRNA Genome size 7.5kb 9.6kb 7.2kb TransmissionFecal-oral Parenteral Chronic disease Never Yes Nevel Vaccine Yes No Yes Oncogenic Yes No

Characteris%cs'of'Hepa%%s'Viruses' Family'''''''''''''''''Picornaviridae''''''''''''''''''''''Flaviviridae'''''''''''''''''''''''''''Hepeviridae'''''''''''''''''''''' Virus''''''''''''''''''''''Hepa%%s'A'''''''''''''''''''''''Hepa%%s'C'''''''''''''''''''''''''''''''''Hepa%%s'E' Envelope''''''''''''''''No'''''''''''''''''''''''''''''''''''''''''Yes''''''''''''''''''''''''''''''''''''''''''''No' Genome'''''''''''''''+'ssRNA''''''''''''''''''''''''''''''''+'ssRNA'''''''''''''''''''''''''''''''''''+'ssRNA''''''''''''''''''''' Genome'size'''''''''''7.5kb''''''''''''''''''''''''''''''''''9.6kb''''''''''''''''''''''''''''''''''''''''''7.2kb''''''''''''''''''''''''''''''''''' Stability''''''''''''''''Heat'and'acidNstable''''''''''EtherNsensi%ve,'''''''''''''''''''HeatNstable''' '''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''acidNsensi%ve''''''''' Transmission'''''''''''FecalNoral'''''''''''''''''''''Parenteral'''''''''''''''''''''''''''''''''FecalNoral' Chronic'disease'''''''''Never''''''''''''''''''''''''''''Yes'''''''''''''''''''''''''''''''''''''''''''''''Never' Vaccine''''''''''''''''''''''''Yes''''''''''''''''''''''''''''''''''No''''''''''''''''''''''''''''''''''''''''''''''''Yes' Oncogenic''''''''''''''''''No''''''''''''''''''''''''''''''''''Yes''''''''''''''''''''''''''''''''''''''''''''''''No

Characteristics of Hepatitis Viruses Viru Hepatitis A Hepatitis c Hepatitis e Family Picornaviridae Flaviviridae Hepeviridae Envelope No Stability Heat and acid-stable Ether-sensitive Heat-stable acid-sensitive Genome + SSRNA + ssrna ssRNA Genome size 7.5kb 9.6kb 7.2kb Transmission Fecal-oral Parenteral Chronic disease Never Yes Nevel Vaccine Yes No Yes Oncogenic Yes No

Characteris%cs'of'Hepa%%s'Viruses' Family'''''''''''''''''Picornaviridae''''''''''''''''''''''Flaviviridae'''''''''''''''''''''''''''Hepeviridae'''''''''''''''''''''' Virus''''''''''''''''''''''Hepa%%s'A'''''''''''''''''''''''Hepa%%s'C'''''''''''''''''''''''''''''''''Hepa%%s'E' Envelope''''''''''''''''No'''''''''''''''''''''''''''''''''''''''''Yes''''''''''''''''''''''''''''''''''''''''''''No' Genome'''''''''''''''+'ssRNA''''''''''''''''''''''''''''''''+'ssRNA'''''''''''''''''''''''''''''''''''+'ssRNA''''''''''''''''''''' Genome'size'''''''''''7.5kb''''''''''''''''''''''''''''''''''9.6kb''''''''''''''''''''''''''''''''''''''''''7.2kb''''''''''''''''''''''''''''''''''' Stability''''''''''''''''Heat'and'acidNstable''''''''''EtherNsensi%ve,'''''''''''''''''''HeatNstable''' '''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''acidNsensi%ve''''''''' Transmission'''''''''''FecalNoral'''''''''''''''''''''Parenteral'''''''''''''''''''''''''''''''''FecalNoral' Chronic'disease'''''''''Never''''''''''''''''''''''''''''Yes'''''''''''''''''''''''''''''''''''''''''''''''Never' Vaccine''''''''''''''''''''''''Yes''''''''''''''''''''''''''''''''''No''''''''''''''''''''''''''''''''''''''''''''''''Yes' Oncogenic''''''''''''''''''No''''''''''''''''''''''''''''''''''Yes''''''''''''''''''''''''''''''''''''''''''''''''No

HEPATITIS A and virus(HAv) Hepatitis a is caused by infection with the hepatitis a virus, which is an RNa virus in the picornavirus family Only one single serotype worldwide has been discovered. unlike some other viruses that have subtypes Acute disease and asymptomatic infection No chronic infection Protective antibodies develop in response to infection confers lifelong immunity

HEPATITIS A and Virus(HAV) • Hepatitis A is caused by infection with the hepatitis A virus, which is an RNA virus in the picornavirus family. • Only one single serotype worldwide has been discovered, unlike some other viruses that have subtypes. • Acute disease and asymptomatic infection • No chronic infection • Protective antibodies develop in response to infection - confers lifelong immunity

27nm icosahedral. first identified in 1973 HAV (7478 bases) 27 VPg

HAV' 27nm,'icosahedral.'First'iden%fied'in'1973

Stability of HAV Stable to ac at pH 3 Solvents(ether, chloroform) detergents salt water, groundwater(months) drying(stable) temperature 4℃: weeks 56C for 30minutes stable 61 Cfor 20minutes: partial inactivation Extra precautions in dealing with HAV products

Stability of HAV Stable to: acid at pH 3 Solvents (ether,chloroform) detergents salt water, groundwater(months) drying(stable) temperature 4℃: weeks 56℃for 30minutes: stable 61℃for 20minutes: partial inactivation * Extra precautions in dealing with HAV products !

Stability of HAV Inactivated by chlorine treatment of drinking water formalin(0.35%, 37C, 72hours) acetic acid(2%, 4hours) B-propiolactone(0.25% Thours)(β-乙丙内酯) Ultraviolet radiation (2u w/cm/min autoclaving, boiling in water for 5 minutes dry heat at 1 80 for l hour

Stability of HAV Inactivated by: chlorine treatment of drinking water formalin(0.35%, 37℃, 72hours) acetic acid(2%, 4hours) B-propiolactone (0.25%,1hours)（β-乙丙内酯） Ultraviolet radiation (2µW/㎝2/min) Autoclaving, boiling in water for 5 minutes, dry heat at 180 for 1 hour

HAV infection 1. Entry into mouth 2. Viral replication in the liver 3. Virus present in blood and feces 10-12 Oral days after infection acquisition 4. Virus excretion may continue for up to 3 weeks after onset of symptoms crosses nosta sBlood Bile Stool

1. Entry into mouth 2. Viral replication in the liver 3. Virus present in blood and feces 10-12 days after infection 4. Virus excretion may continue for up to 3 weeks after onset of symptoms HAV'infec%on