复旦大学：《医学微生物学 Medical Microbiology（MBBS）》课程讲义_理论课_Lecture7-Pyogenic coccus

PYOGENIC COCCI Staphylococcus, Streptococcus, Neisseria Junqi zhang(张俊琪),PhD MOH&MOE Key Lab of Medical Molecular Virology Shanghai Medical College, Fudan University (复旦大学上海医学院分子病毒学教育部/卫生部重点实验室

PYOGENIC COCCI Staphylococcus, Streptococcus, Neisseria Junqi Zhang (张俊琪), PhD MOH&MOE Key Lab of Medical Molecular Virology Shanghai Medical College, Fudan University (复旦大学上海医学院分子病毒学教育部/卫生部重点实验室)

Cocci 酪沙 Acc V. Spot Magn Det WD 300kv1010000xSE8 83 staph97-11-49 Streptococcus Staphylococcus Neisseria

Cocci Staphylococcus Streptococcus Neisseria

Smear Gram Stain sS

Smear & Gram Stain

Staphylococcus Pathogenic Cocci Streptococcus Gram stain (yogenic Cocci) Positive Enterococcus Neisseria Gram stain Negative

Pathogenic Cocci (Pyogenic Cocci) Staphylococcus Streptococcus Enterococcus Neisseria Gram stain Positive Gram stain Negative

Staphylococcus >40 species Staph=grape-like clusters coccus=round Scanning electron microscope

Staphylococcus >40 species Staph=grape-like clusters coccus=round Scanning Electron Microscope

General Characteristics of the staphylococci Include a major human pathogen and skin commensals Spherical cells arranged in irregular clusters 产数学 v Gram-positive Coagulase: an important indicator t of pathogenic staphylococci Lack spores and flagell May have capsules Gram staining

General Characteristics of the Staphylococci  Include a major human pathogen and skin commensals  Spherical cells arranged in irregular clusters  Gram-positive  Coagulase: an important indicator of pathogenic staphylococci  Lack spores and flagella  May have capsules Gram staining

Classification I: isolate character on blood agar plate eta-hemolysis caused by a-toxin Zone of hemolysis caused by B-toxin ig ☆S. aureus Sepidermidis S saprophyticus

 S.aureus S.epidermidis S. saprophyticus Classification I: isolate character on blood agar plate

Classification l: coagulase coagulase positive staphylococcus coagulase negative staphylococcus, CNS S epidermidis S saprophyticus Coagulase: an important indicator of pathogenic staphylococcus

 coagulase positive staphylococcus S. aureus coagulase negative staphylococcus, CNS S. epidermidis S. saprophyticus Coagulase: an important indicator of pathogenic staphylococcus Classification II: coagulase

aureus 雙 :额想 你 v Morphology culture, and biological characteristics Y Pathogenesis: virulent factors dr Iseases Diagnostic laboratory tests v Principles of controlling staphylococcal infections

S. aureus  Morphology, culture, and biological characteristics  Pathogenesis: virulent factors, diseases  Diagnostic laboratory tests  Principles of controlling staphylococcal infections

Culture characteristics -s aureus V Facultative anaerobe Y Grows in large, round, opaque golden yellow colonies; B- hemolysis on blood agar plate Optimum temperature of 37oC v Tolerate high salt: 10%0-15% NaCl, high temperatures: 600C lh:80°30min Y Withstands extremes in pH Drug resistance: mrsa

Culture Characteristics - S. aureus  Facultative anaerobe  Grows in large, round, opaque, golden yellow colonies; β- hemolysis on blood agar plate  Optimum temperature of 37oC  Tolerate high salt: 10%-15% NaCl, high temperatures: 60oC 1h; 80oC 30min  Withstands extremes in pH  Drug resistance: MRSA