复旦大学：《医学微生物学 Medical Microbiology（MBBS）》课程讲义_理论课_Lecture5-anti-infection-vaccine

Anti-microbe immunity -Immunity to Bacteria DiQu(瞿涤) MOH&MOE Key Lab of Medical Molecular Virology Shanghai Medical College, Fudan University 复旦大学基础医学院 医学分子病毒学教育部卫生部重点实验室

Anti-microbe immunity -Immunity to Bacteria Shibo Jiang (姜世勃) MOH&MOE Key Lab of Medical Molecular Virology Shanghai Medical College, Fudan University 复旦大学上海医学院分子病毒学教育部/卫生部重点实验室 Di Qu (瞿涤) MOH&MOE Key Lab of Medical Molecular Virology Shanghai Medical College, Fudan University 复旦大学基础医学院 医学分子病毒学教育部/卫生部重点实验室

Outcome of Infection ·| nfection wins→ death or chronic infection Infection loses -live(innate adaptive) Studied intensely Best outcome is Infections killed quickly(innate only Not well studied How is it that most people are not perpetually sick? (Innate immunity squelching most infections

Outcome of Infection • Infection wins death or chronic infection • Infection loses live (innate & adaptive) Studied intensely Best outcome is • Infections killed quickly (innate only) Not well studied How is it that most people are not perpetually sick? (Innate immunity squelching most infections)

Key Words Extracelluar pathogen Intracelluar pathogen Innate immunity Adaptive immunity Immunity to extracelluar pathogen Immunity to intracelluar pathogen

Key Words Extracelluar pathogen Intracelluar pathogen Innate immunity Adaptive immunity Immunity to extracelluar phathogen Immunity to intracelluar pathogen

Where to find the pathogen in cells? Extracellular versus Intracellular pathogen Extracellular pathogen capable of replicating outside of the host cells a destroyed when phagocytosed a damaging tissues as they remain outside cells inducing the production of opsonizing antibodies usually cause acute diseases of relatively short duration Intracellular pathogen a Able to multiply within phagocytes a frequently cause chronic disease BC

Where to find the pathogen in cells? Extracellular versus Intracellular pathogen  Extracellular pathogen  capable of replicating outside of the host cells  destroyed when phagocytosed  damaging tissues as they remain outside cells  inducing the production of opsonizing antibodies usually cause acute diseases of relatively short duration  Intracellular pathogen  Able to multiply within phagocytes  frequently cause chronic disease BC

Penetration and spread Gut lumen Epithelium Vibrio cholerae Salmonella enteritidis Salmonella typhi Blood stream

Penetration and spread Vibrio cholerae Salmonella enteritidis Salmonella typhi Epithelium Gut lumen Blood stream

Intracellular bacteria Macrophage or neutrophil Lysozome Bacteria Phagosome Phagolysosomes No fusion Fusion Enter cytoplasm

Intracellular bacteria No fusion Lysozome Phagosome Fusion Enter cytoplasm Bacteria Macrophage or neutrophil Phagolysosomes 6

Intracellular bacteria Listeria cytoplasm Shigella Endosomes Phagolysosomes Salmonella Legionella Mycobacteria Chlamydia lysosomes Phagosome

Endosomes Phagosomes lysosomes Phagolysosomes Legionella Chlamydia Listeria Shigella Sammonella Mycobacteria Intracellular bacteria cytoplasm 7

Balance between Infection and Immunity Pathogen Host infection immunity Virulence VS Host-resistance -immunity Bolus of infection x virulence Disease s immunity

Pathogen infection Host immunity Bolus of infection x virulence immunity Disease = Host-resistance -immunity Balance between Infection and Immunity Virulence vs

Protective Immunity To Bacteria Defense against microbes is mediated by Innate immunity and acquired immunity Humoral immunity(antibodies) Through both Cell mediated immunity( CM

Protective Immunity To Bacteria Defense against microbes is mediated by: Innate immunity and acquired immunity Humoral immunity (antibodies) Through both Cell mediated immunity (CMI)

Time Table of immune responses Against re-infection Innate immunigy Recognition by Removal of immediate: Infection reformed 0-4 hours) nonspecific effectors infectious agent Early induced T Recognition of Inflammation Infection microbial-associated,> recruitment and Removal of response activation of (early: 4-96 hours) molecular patter infectious agent effector cells Adaptive immune Transport of Recognition Clonal expansion Removal response Infection tigen to by naive and differentiation of (ate: >96 hours) ymphoid organs B and T cells to effector cells infectious agent Figure 2-1 Immunobiology, 6/e ( Garland Science 2005)

Figure 2-1 Time Table of immune responses Against re-infection?