《微生物生理学》课程教学课件（讲稿）第三章 产能代谢 Energy Yielding Metabolism（2/7）

第一节能量来自有机化合物 碳水化合物 烃 二碳、一碳化合物 内源性储藏物质 含氨化合物 脂肪

第一节 能量来自有机化合物 碳水化合物 烃 二碳、一碳化合物 内源性储藏物质 含氮化合物 脂肪

(2)能量的产生 CH2O -[H] 经呼吸链 ①呼吸 2 A H2O -[H] 1②无氧 NO,SO,CO 呼吸 B- NO:,SO-,CH -H] A,B或C一 发醇 AH,BH,或CH -H] (发酵产物：乙醇、乳酸等) C0: 脱氢 递氢 受氢

(2) 能量的产生

Energized 多4+4+十+十十十十十十以 A membrane B P ADP +Pi- B~P -ATP C~P Less energized ADP membrane D+ATP (a)Substrate-level phosphorylation (b)Oxidative phosphorylation

CH2=C-COO NH2 CHO High energy Low energy HCOH Low energy High energy anhydride bond ester bond OHCH ester bond anhydride HCOH Phosphoenolpyruvate -O-CH2 HCOH CH2-0-P-O Acetyl phosphate OHOH Glucose 6-phosphate Adenosine triphosphate(ATP) Compound G0kJ/mol High energy Phosphoenolpyruvate -51.6 1,3-Bisphosphoglycerate -52.0 CH-6-s-(CM.-N-G-(CH)-N-C-(CH)-o-R Acetyl phosphate -44.8 ATP -31.8 ADP -31.8 Acetyl B-Mercapto Pantothenic acid ethylamine Low energy AMP -14.2 Acetyl-CoA Glucose 6-phosphate -13.8

(2)能量的产生 1、呼吸链 2、有氧呼吸 3、无氧呼吸 4、发酵

(2) 能量的产生 １、呼吸链 ２、有氧呼吸 ３、无氧呼吸 ４、发酵

1、呼吸链（电子传递链) 真核生物的呼吸链 原核生物的呼吸链

真核生物的呼吸链 原核生物的呼吸链 1、呼吸链(电子传递链)

4H' 4H 2H' Intermembrane Space FeS.-Cytc- 28 Cyt as FMN FeS NADH NAD FAD +H中 4H 4H' 202+2HH0 -2H Succinate Fumarate Matrix

真核生物呼吸链的蛋白质组成 Protein Components of the Mitochondrial Electron-Transfer Chain Mass Number of Prosthetic Enzyme complex (kDa) subunits* group(s) I NADH 850 42(14) FMN,Fe-S dehydrogenase lI Succinate 140 5 FAD,Fe-S dehydrogenase IlI Ubiquinone: 250 11 Hemes,Fe-S cytochrome c oxidoreductase Cytochrome ct 13 1 Heme IV Cytochrome 160 13(3-4) Hemes;CuA,CuB oxidase *Numbers of subunits in the bacterial equivalents in parentheses. Cytochrome c is not part of an enzyme complex;it moves between Complexes Ill and IV as a freely soluble protein

真核生物呼吸链的蛋白质组成

CHs CHsO. (CH2-CH-C-CH2)1o-H Ubiquinone (Q) (fully oxidized) CH3O 辅酶Q CH3 H' +e 0 CHO R Semiquinone radical (QH) CHO CH3 OH H*+e OH CH2O R Ubiquinol (QH2) (fully reduced) CHsO CH3 OH

辅酶 Q

Standard Reduction Potentials of Respiratory Chain and Related Electron Carriers Redox reaction(half-reaction) E'(V) 2H+2e→H2 -0.414 NAD+H+2E→NADH -0.320 NADP++H+2E→NADPH -0.324 NADH dehydrogenase (FMN)+2H'+2e-NADH dehydrogenase(FMNH2) -0.30 Ubiquinone 2H*+2e ubiquinol 0.045 Cytochrome b(Fe)+ecytochrome b(Fe2) 0.077 Cytochrome c(Fe3)+ecytochrome c (Fe2+) 0.22 Cytochrome c(Fe3)+ecytochrome c(Fe2) 0.254 Cytochrome a(Fe3)+ecytochrome a(Fe2*) 0.29 Cytochrome a (Fe3)+ecytochrome aa (Fe2") 0.55 202+2H+2e→H,0 0.816