《微生物生理学》课程教学课件（讲稿）第三章 产能代谢 Energy Yielding Metabolism（7/7）

Chemicals ↓ Chemotrophy Phototrophy Organic Inorganic chemicals chemicals (glucose,acetate,etc.) (H2,H2S,Fe2+,NH4",etc.) Chemoorganotrophs Chemolithotrophs Phototrophs (glucose +2-CO2+H2O)(H2+02-H2O)(lightATP) ATP ATP

第三节能量来自光 产氧光合作用 不产氧光合作用 以视紫红质为基础的光合作用

第三节 能量来自光 产氧光合作用 不产氧光合作用 以视紫红质为基础的光合作用

Table 9.5 Diversity of Phototrophic Organisms Eucaryotic Organisms Procaryotic Organisms Plants Cyanobacteria Multicellular green,brown, Green sulfur bacteria and red algae Green nonsulfur bacteria Unicellular protists Halobacterium (archaeon) (e.g.,euglenoids, Purple sulfur bacteria dinoflagellates,diatoms) Purple nonsulfur bacteria Prochloron oxygenic photosynthesis-eucaryotes and cyanobacteria anoxygenic photosynthesis -all other bacteria

oxygenic photosynthesis – eucaryotes and cyanobacteria anoxygenic photosynthesis – all other bacteria Table 9.5

Chlorophyll-based phototrophy Light Chlorophyll or Electron transport chain NAD(P)+ bacteriochlorophyll NAD(P)H photo phos PMF ATP Carbon source (often CO2) Biosynthesis Rhodopsin-based phototrophy Light Bacteriorhodopsin photo phos (archaeorhodopsin) PMF ATP proteorhodopsin Carbon source 。Biosynthesis

CH H 0.9 0.8 Chlorophyll a Green alga C, peaks Chlamydomonas 0.7 Carotenoid 0 peak 衣藻 HgC 05 H 0.4 H2C H 0.3 CH2 H COOCH3 0.2 Cyclopentanone COOC20H39 ring 0.1 Phytol 0 340400 500600700 800900 Chlorophyll a Wavelength(nm) 0.9 Bacteriochlorophyll a peaks H HC H 8 Carotenoid 、H 0.7 peaks 0.6 Purple bacterium 0.5 Rhodopseudomona H C H CH3 0.3 红假单胞菌 H2C H CH2 HT d 0.2 COOCH 0.1 Cyclopentanone COOC2oH39 ring 0 Phytol 40400500600700800900 Wavelength(nm) Bacteriochlorophyll a

Green alga Chlamydomonas Purple bacterium Rhodopseudomona s 红假单胞菌 衣藻

hv 一个光合作用的单位 由200-300个叶绿素 分子组成 RC RC RC RC RC RC RC

一个光合作用的单位 由200-300个叶绿素 分子组成

一、产氧光合作用 光系统I(photosystem I) P700 光系统IⅡ(photosystemⅡ) P680

一、产氧光合作用 光系统Ⅰ(photosystem Ⅰ) P700 光系统Ⅱ(photosystem Ⅱ) P680

-1.4 Reaction center Q1 00 -1.2 -1.0 绿色植物的光合作用 A or Fes noncyclic -0.8 P'680 Fes electron flow ATP NADP -0.6 Fd铁氧还蛋白 Pheo.a made(noncy是 Pyridine nucleotide -0.4 reductase(FAD) photophos- phorylation) NADP+ -0.2 Cyt b 563 PSI Q 2e 0.0 ADP+P 2 photons PO ATP cyclic electrol +0.2 Cyt be flow-ATP PSII FeS Cyf→PC2e made(cyclic +0.4 Antenna 2e photophos- P700 (Noncyclic) +0.6 2 photons phorylation) OEC +0.8 H20 2e Mn2+3+ 22e +1.0 202+2H P680

noncyclic electron flow – ATP + NADPH made (noncyclic photophos￾phorylation) cyclic electron flow – ATP made (cyclic photophos￾phorylation) 绿色植物的光合作用 铁氧还蛋白

electron flow→PMF→ATP Chloroplast ADP+P ATP Thylakoid Stroma 类襄体 基质 基质 Stroma Pyridine nucleotide Photons Photons reductase Cytochrome 2 bf complex 2NADP 2NADPH nthase 8H OEC -PC-Cu' 8H PC-CU Photosystem I 2H0 Photosystem ll O,+4H' 类囊体腔 Thylakoid lumen

Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. electron flow → PMF → ATP 类囊体 基质 基质 类囊体腔

颤蓝细菌 (Oscillatoria limnetica) FIGURE 15.20 Cells of the filamentous cyanobacterium Os- cillatoria limnetica grown anaerobically on sulfide as photosyn- thetic electron donor.Note the globules of elemental sulfur, the oxidation product of sulfide,formed outside the cells

颤蓝细菌 (Oscillatoria limnetica)