武汉大学生命科学学院：《分子生物学》（英文版）Part II：Maintenance of the Genome CHAPTER 8 The replication of DNA

Welcome each of You to My molecular Biology Class @或博共字 Wuhan University

Welcome Each of You to My Molecular Biology Class

Molecular biology of the Gene 5/E - Watson et al. ( 2004) Part l: chemistry and genetics Part l: Maintenance of the Genome Part l: Expression of the Genome Part IV: Regulation Part v: Methods 刻博来字 Wuhan Universit

Molecular Biology of the Gene, 5/E --- Watson et al. (2004) Part I: Chemistry and Genetics Part II: Maintenance of the Genome Part III: Expression of the Genome Part IV: Regulation Part V: Methods

Part ll: Maintenance of the genome Dedicated to the structure of DNa and the processes that propagate, maintain and alter it from one cell generation to the next @博共字 Wuhan University

Part II: Maintenance of the Genome Dedicated to the structure of DNA and the processes that propagate, maintain and alter it from one cell generation to the next

Ch 6: The structures of dna and rna Ch 7: Chromosomes, chromatins and the nucleosome ch 8: The replication of DNA Ch 9: The mutability and repair of DNA Ch 10: Homologous recombination at the molecular level Ch 11: Site-specific recombination and transposition of DNA

Ch 6: The structures of DNA and RNA Ch 7: Chromosomes, chromatins and the nucleosome Ch 8: The replication of DNA Ch 9: The mutability and repair of DNA Ch 10: Homologous recombination at the molecular level Ch 11: Site-specific recombination and transposition of DNA

● Molecular Biology course CHAPTER 8: The replication Of dna direction of leading strand polymerase movement leading strand wA1DAWA14 overall direction of DNA replication RNA primer DNA polymerse Okazaki fragments lagging strand direction of lagging strand polymerase movement ted DNA cated DNA @博来字 Wuhan Universit

CHAPTER 8: The replication of DNA •Molecular Biology Course

CHATER 8 The replication of na Teaching Arrangement Watch animation-Understand replication a Go through structural tutorial- Experience the BEAUTY of the nature a Lecture-comprehensive understanding of Chapter 8 Summary and highlight Key points

Teaching Arrangement ◼ Watch animation-Understand replication ◼ Go through structural tutorial￾Experience the BEAUTY of the nature ◼ Lecture-comprehensive understanding of Chapter 8 ◼ Summary and highlight Key points CHAPTER 8 The replication of DNA

CHATER 8 The replication of na The Chemistry of DNA Synthesis >The Mechanism of DNA Polymerase > The Replication Fork The Specialization of DNA General Polymerases DNA Synthesis at the Replication Fork > Initiation of DNA Replication > Binding and Unwinding Detailed >Finishing Replication

The Chemistry of DNA Synthesis ➢ The Mechanism of DNA Polymerase ➢ The Replication Fork ➢ The Specialization of DNA Polymerases ➢ DNA Synthesis at the Replication Fork ➢ Initiation of DNA Replication ➢ Binding and Unwinding ➢ Finishing Replication General Detailed CHAPTER 8 The replication of DNA

CHATER 8 The replication of na The first part describes the basic chemistry of DNA synthesis and the function of the dna polymerase 劣溪字 Wuhan University

The first part describes the basic chemistry of DNA synthesis and the function of the DNA polymerase CHAPTER 8 The replication of DNA

CHAPTER 8 The replication of ona The chemistry of dNa DNA synthesis requires deoxynucleoside triphosphates and a primer:template junction DNA is synthesized by extending the 3 end of the primer Hydrolysis of pyrophosphate(Ppi)is the driving force for DNA synthesis 劣溪字 Wuhan University

The Chemistry of DNA CHAPTER 8 The replication of DNA ➢ DNA synthesis requires deoxynucleoside triphosphates and a primer:template junction ➢ DNA is synthesized by extending the 3’ end of the primer ➢ Hydrolysis of pyrophosphate (PPi) is the driving force for DNA synthesis

base(A, G, C, or T) HO一P—0—P-0—P—0—CH OH b annealed primer growing end of DNA 50 OH 3 template strand Ac GTCGACGIT C GAco IT C GACGT 3 HO ⊙5 dsDNA SSDNA Figure 8-3 Substrates required for DNA synthesis

Figure 8-3 Substrates required for DNA synthesis