武汉大学生命科学学院：《分子生物学》（英文版）sectionG

Quiz for Section G(25 minutes) English answer only 1. What is "dna cloning"? how could you make a genomic library cloning?(30 points) 2. What is subcloning? What are the purposes of subcloning(15 points 3. Please describe all the steps when you do a mini- prepararation of plasmid dNA using alkaline lysis method. Please indicate the roles of each component in lysis solution and neutralization solution. (30 points 4. Why do you add ethidium bromide when you use CsCl density gradient centrifugation to purify your supercoiled plasmid DNA? (10 points) 5. Why do you need to use alkaline phosphatase to treat your restricted dNA fragment when you do cloning and subcloning?(15 points)

1. What is “DNA cloning”? how could you make a genomic library cloning? (30 points) 2. What is “subcloning”? What are the purposes of subcloning. (15 points) 3. Please describe all the steps when you do a mini￾prepararation of plasmid DNA using alkaline lysis method. Please indicate the roles of each component in lysis solution and neutralization solution. (30 points) 4. Why do you add ethidium bromide when you use CsCl density gradient centrifugation to purify your supercoiled plasmid DNA? (10 points) 5. Why do you need to use alkaline phosphatase to treat your restricted DNA fragment when you do cloning and subcloning? (15 points) Quiz for Section G (25 minutes) English answer only