武汉大学生命科学学院：《分子生物学》（英文版）Part II：Maintenance of the Genome CHAPTER11 Site-Specific Recombination & Transposition of DNA ?Molecular Biology Course

Molecular biology of the Gene 5/E - Watson et al. ( 2004) Part I: Chemistry and Genetics Part l: Maintenance of the Genome Part l: Expression of the Genome Part IV: Requlation Part v: methods

Molecular Biology of the Gene, 5/E --- Watson et al. (2004) Part I: Chemistry and Genetics Part II: Maintenance of the Genome Part III: Expression of the Genome Part IV: Regulation Part V: Methods

Part ll: Maintenance of the genome Dedicated to the structure of DNA and the processes that propagate, maintain and alter it from one cell generation to the next 刻博来字 Wuhan Universit

Part II: Maintenance of the Genome Dedicated to the structure of DNA and the processes that propagate, maintain and alter it from one cell generation to the next

Ch 6: The structures of dna and rna Ch 7: Chromosomes, chromatins and I the nucleosome Ch 8: The replication of DNA Ch 9: The mutability and repair of DNA Ch 10: Homologous recombination at the molecular level Ch 11: Site-specific recombination and transposition of DNA 刻博来字 Wuhan Universit

Ch 6: The structures of DNA and RNA Ch 7: Chromosomes, chromatins and the nucleosome Ch 8: The replication of DNA Ch 9: The mutability and repair of DNA Ch 10: Homologous recombination at the molecular level Ch 11: Site-specific recombination and transposition of DNA

Molecular Biology Course Chapter 11 Site-Specific Recombination transposition of DNA @或属字 Wuhan University

Chapter 11 Site-Specific Recombination & Transposition of DNA •Molecular Biology Course

Although DNA replication, repair, homologous recombination occur with high fidelity to ensure the genome identity between generations, there are genetic processes that rearrange dNA sequences and thus lead to a more dynamic genome structure

Although DNA replication, repair, homologous recombination occur with high fidelity to ensure the genome identity between generations, there are genetic processes that rearrange DNA sequences and thus lead to a more dynamic genome structure

Two classes of genetic recombination for DNA rearrangement: Conservative site-specific recombination (CSSRE recombination between two defined sequence elements Transpositional recombination (Transposition: recombination between specific sequences and nonspecific DNA sites

Two classes of genetic recombination for DNA rearrangement: • Conservative site-specific recombination (CSSR): recombination between two defined sequence elements • Transpositional recombination (Transposition): recombination between specific sequences and nonspecific DNA sites

Figure 11-1 B site-specific recombination sites recombination transposable element transposition Copyright o 2004 Pearson Education, Inc, publishing as Benjamin Cummings

Figure 11-1

OUTLINE 1. Conservative Site-Specific Recombination 2. Biological Roles of Site-Specific Recombination(n phage integration/excision multimeric genome resolution 3. Transposition concepts, learning from B McClintock, DNa tranposons. Viral-like retrotransposons/retroviruses, poly-A retrotransposons)

OUTLINE 1. Conservative Site-Specific Recombination 2. Biological Roles of Site-Specific Recombination (l phage integration/excision, multimeric genome resolution) 3. Transposition ( concepts, learning from B. McClintock, DNA tranposons. Viral-like retrotransposons/retroviruses, poly-A retrotransposons)

Topic 1: Conservative Site- Specific Recombination 1. Exchange of non-homologous sequences at specific DNA sites (what 2. Mediated by proteins that recognize specific DNA sequences. (how)

Topic 1: Conservative Site￾Specific Recombination 1.Exchange of non-homologous sequences at specific DNA sites(what) 2.Mediated by proteins that recognize specific DNA sequences. (how)

1-1 Site-specific recombination occurs at 2 specific DNA sequences in the target DNA a CSSR(conserved site-specific recombination)is responsible o for many reactions in which a 8 defined segment of dNa is rearranged

◼ CSSR (conserved site-specific recombination) is responsible for many reactions in which a defined segment of DNA is rearranged. Conservative Site -Specific Recombination 1-1 Site-specific recombination occurs at specific DNA sequences in the target DNA