武汉大学生命科学学院：《分子生物学》（英文版）sectionK-N

Quiz: Section K, L, M and N, 40 min, English answer only 1. Please list the important cis-acting elements and transacting enzymes/factors in prokaryotic transcription including initiation, elongation and termination, and briefly describe their corresponding roles (35 point 2. Please briefly describe how 力E coli regulates the LacZy A RNa synthesis in order to uses lactose as carbon source when there is no glucose present. (20 points 3. What are the corresponding genes transcribed by three different RNa polymerases in eukaryotes? Are these Rna Pol more complex than prokaryotic RNa pol? Why(10 points)

Quiz: Section K, L, M and N, 40 min, English answer only 1. Please list the important cis-acting elements and transacting enzymes/factors in prokaryotic transcription including initiation, elongation and termination, and briefly describe their corresponding roles (35 point) 2. Please briefly describe how E. coli regulates the LacZYA RNA synthesis in order to uses lactose as carbon source when there is no glucose present. (20 points) 3. What are the corresponding genes transcribed by three different RNA polymerases in eukaryotes? Are these RNA Pol more complex than prokaryotic RNA pol? Why (10 points)

4. It is known that HIv uses RNA Polymerase Ii in mammalian cells to synthesize the viral rna Please describe(1)RNa Polymerase Ii catalyzed transcription initiation; (2)how HIV transcribes full length viral rna by interfering with this process. (30 points) 5. Regulation of transcription in eukaryotes is largely achieved by the domain structure of transcription factors. please describe four major domain structures and how they contribute to transcription regulation(20 points)

4. It is known that HIV uses RNA Polymerase II in mammalian cells to synthesize the viral RNA. Please describe (1) RNA Polymerase II catalyzed transcription initiation; (2) how HIV transcribes full length viral RNA by interfering with this process. (30 points) 5. Regulation of transcription in eukaryotes is largely achieved by the domain structure of transcription factors. Please describe four major domain structures and how they contribute to transcription regulation (20 points)