武汉大学生命科学学院：《分子生物学》（英文版）2004Quiz4

● Molecular iology course Quiz roles (1)桌上只许有笔,答题纸和英文课本。(2)不许 离开自己的座位。(3)不许偷看,不许讲话(有问题 举手)(4)老师宣布停的时候,必须放下自己的笔。 (5)老师宣布收试卷时,试卷必须快速由每排的最左 边传向右边,每排最右边的同学负责把试卷交给当堂 助教。 如果主讲老师或助教发现任何学生违反以上任何 条规则,第一次违规者,扣除总考试成绩的15% 第二次违规者必须重修这们课。 @劣博字 Wuhan Universit

Quiz Roles •Molecular Biology Course （1）桌上只许有笔，答题纸和英文课本。（2）不许 离开自己的座位。（3）不许偷看，不许讲话 (有问题 举手）（4）老师宣布停的时候，必须放下自己的笔。 （5）老师宣布收试卷时，试卷必须快速由每排的最左 边传向右边，每排最右边的同学负责把试卷交给当堂 助教。 如果主讲老师或助教发现任何学生违反以上任何 一条规则，第一次违规者，扣除总考试成绩的15%， 第二次违规者必须重修这们课

Molecular 0logy course Quiz 4: Section M-O 50 min 1. 30)Describe the major differences between the prokaryotic and eukaryotic transcription. Give one example to illustrate that transcription of eukaryotic genes is much more complicated than that of the prokaryotic 2.(20 Why many transcription factors contain DNA-binding domains? Is DNA binding domain alone can exert the activation or repression of the gene expression? Why?

Quiz 4: Section M-O 1. (30) Describe the major differences between the prokaryotic and eukaryotic transcription. Give one example to illustrate that transcription of eukaryotic genes is much more complicated than that of the prokaryotic. 2. (20) Why many transcription factors contain DNA-binding domains? Is DNA binding domain alone can exert the activation or repression of the gene expression? Why? •Molecular Biology Course 50 min

3.(15Which classes of bacterial RNA need to be processed to become functional, and how? 4.(15)What is a ribozyme, list all the ribozymes that involve in RNa processing, and briefly describe their roles in the corresponding RNA processing events 5.(20) It is known that spliceosome carry out pre-mRNA splicing? Describe how the cis acting elements included in the pre-mRNA and the transacting factors included in the spliceosome interact with each other to accomplish the splicing event

3. (15) Which classes of bacterial RNA need to be processed to become functional, and how? 4. (15) What is a ribozyme, list all the ribozymes that involve in RNA processing, and briefly describe their roles in the corresponding RNA processing events. 5. (20) It is known that spliceosome carry out pre-mRNA splicing? Describe how the cis￾acting elements included in the pre-mRNA and the transacting factors included in the spliceosome interact with each other to accomplish the splicing event

6. One of the many surprises to come from the various genome projects is that the number of genes an organism has is not necessarily a good predictor of its complexity. For example, humans are clearly much more complex than nematode worms, yet only have about 50% more genes. How could splicing help resolve this paradox?(20 points)

6. One of the many surprises to come from the various genome projects is that the number of genes an organism has is not necessarily a good predictor of its complexity. For example, humans are clearly much more complex than nematode worms, yet only have about 50% more genes. How could splicing help resolve this paradox? (20 points)

O insertion point 100 5 0 5 0 15 21 size of insert(bp)