武汉大学生命科学学院：《分子生物学》（英文版）sectionH-J

Quiz: Section H, i and J, 30 min english answer only 1. What are the essential elements that a cloning vector should have how about the expression vectors and the integration vectors? What is a shuttle vector and please describe its importance? (35) 2. How could you generate a genomic library and CDNA library for a given type of human cells Will you choose the same vector for these two different libraries ?(35) 3. If you have purified a protein factor interacting with DNA Pol a in the same type of human cell as in2",but you do not know any thing else about the new protein Then you have the purified protein sequenced from its 5 end, and get a polypeptide sequence containing 7 amino acids. What are you going to do to identify the clone encoding the new protein from the cDNA library that you constructed in 2 Then what are you going to do to analyze the identified clone?(35)

Quiz: Section H, I and J, 30 min, English answer only 1. What are the essential elements that a cloning vector should have? how about the expression vectors and the integration vectors? What is a shuttle vector and please describe its importance? (35) 2. How could you generate a genomic library and cDNA library for a given type of human cells ? Will you choose the same vector for these two different libraries? (35) 3. If you have purified a protein factor interacting with DNA Pol a in the same type of human cell as in “2”, but you do not know any thing else about the new protein. Then you have the purified protein sequenced from its 5’ end, and get a polypeptide sequence containing 7 amino acids. What are you going to do to identify the clone encoding the new protein from the cDNA library that you constructed in “2”? Then what are you going to do to analyze the identified clone? (35)