武汉大学生命科学学院：《分子生物学》（英文版）Part IV：Regulation CHAPTER16 Regulation in prokaryotes

Molecular biology of the Gene 5/E - Watson et al. ( 2004) Part l: chemistry and genetics Part l: Maintenance of the Genome Part l: Expression of the Genome Part Iv: Regulation Part v: Methods 2005-5-10 刻博来字 Wuhan Universit

2 Molecular Biology of the Gene, 5/E --- Watson et al. (2004) Part I: Chemistry and Genetics Part II: Maintenance of the Genome Part III: Expression of the Genome Part IV: Regulation Part V: Methods 2005-5-10

Part IV Regulation Ch16: Requlation in prokaryotes Ch 17: Regulation in eukaryotes Ch 18: Regulation during development and in diseases(brief introduction Ch 19; Comparative genomics and evolution of anima/ diversity(Not covered in the lecture)

3 Part IV Regulation Ch 16: Regulation in prokaryotes Ch 17: Regulation in eukaryotes Ch 18: Regulation during development and in diseases (brief introduction) Ch 19: Comparative genomics and evolution of animal diversity (Not covered in the lecture)

Expression of many genes in cells are requlated H ed housekeeping genes: expresse constitutively, essential for basic processes involving in cell replication and growth Inducible genes: expressed only when they are activated by inducers or cellular factors

4 Housekeeping genes: expressed constitutively, essential for basic processes involving in cell replication and growth. Inducible genes: expressed only when they are activated by inducers or cellular factors. Expression of many genes in cells are regulated

Chapter 16 Regulation principles and How genes are regulated in bacteria Chapter 17 Basic mechanism of gene expression in eukaryotes Chapter 18 The mechanism of rnai and the role of miRNA in development and cancergenesis

5 Chapter 16 Regulation principles and How genes are regulated in bacteria Chapter 17 Basic mechanism of gene expression in eukaryotes Chapter 18 The mechanism of RNAi and the role of miRNA in development and cancergenesis

Surfing the contents of PartA -The heart of the frontier biologica isciplines

6 Surfing the contents of Part IV --The heart of the frontier biological disciplines

Some of the peoples who significantly contribute to the knowledge of gene regulation

7 Some of the peoples who significantly contribute to the knowledge of gene regulation

Jeff Roberts and Ann Burgess, 1970 Symposium on Christiane Nusslein-Volhard, 1996 CSHL Meeting Transcription of Genetic Material on Zebrafish Development and Genetics. Mutant

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Molecular Biology Course Chapter 16 Gene Regulation in Prokaryotes 隅来字 Wuhan University

10 Chapter 16 Gene Regulation in Prokaryotes •Molecular Biology Course

TOPIC 1 Principles of Transcriptional Regulation [watch the animation TOPIC 2 Requlation of Transcription Initiation: Examples from Bacteria Lac operon, alternative o factors NtrC, MerR, Gal rep, araBAD operon) TOPIC 3 Examples of Gene Regulation after Transcription Initiation(Trp operon) TOPIC 4 The Case of Phage A Layers of Regulation

11 TOPIC 1 Principles of Transcriptional Regulation [watch the animation] TOPIC 2 Regulation of Transcription Initiation: Examples from Bacteria (Lac operon, alternative s factors, NtrC,MerR, Gal rep, araBAD operon) TOPIC 3 Examples of Gene Regulation after Transcription Initiation (Trp operon) TOPIC 4 The Case of Phage λ: Layers of Regulation