清华大学：现代生命科学实验教学中心《生物化学实验》课程教学课件（讲稿）Immunochemical techniques Enzyme-linked immunosorbent assay（ELISA）

ImmunochemicaltechniquesEnzyme-linked immunosorbentassay (ELISA)福动.CLTLS,TsinghuaUniversity

Immunochemical techniques Enzyme-linked immunosorbent assay (ELISA) CLTLS, Tsinghua University

CentrifugationPreparation &ChromatographySeparationBiochemistrySpectroscopicImmunochemicaltechniquesAnalysisElectrophoresisCLTLS,TsinghuaUniversity

Centrifugation Immunochemical techniques Electrophoresis Spectroscopic Analysis Chromatography Biochemistry Preparation & Separation CLTLS, Tsinghua University

Outline·Immunological techniques·Application of Immunochemicaltechniques·Immunoassay&ELiSA·OtherimmunochemicaltechniquesCLTLS,TsinghuaUniversit

Outline • Immunological techniques • Application of Immunochemical techniques • Immunoassay & ELISA • Other immunochemical techniques CLTLS, Tsinghua University

Outline·lmmunologicaltechniquesApplication of ImmunochemicaltechniquesImmunoassay&ELiSAOtherimmunochemicaltechniguesCLTLS,TsinghuaUniversity

Outline • Immunological techniques • Application of Immunochemical techniques • Immunoassay & ELISA • Other immunochemical techniques CLTLS, Tsinghua University

Immunological technigues福CLTLS,TsinghuaUniversity

Immunological techniques CLTLS, Tsinghua University

Immunologicaltechniquesstudytheimmunesystemgenerate or use immunological reagentsImmunochemicaltechniquesantibodies,antigens and theirinteractionscomplementcomponentsCLTLS,TsinghuaUniversit

Immunological techniques • study the immune system • generate or use immunological reagents Immunochemical techniques • antibodies, antigens and their interactions • complement components CLTLS, Tsinghua University

Immunological techniques·Immunological techniques include:·experimentalmethodstostudytheimmunesystem:methods to generate or use immunologicalreagentsas experimentaltools.The most common immunological methods :productionand use of antibodies to detect specificproteins in biologicalsamples.http://www.nature.com/subjects/immunological-techniquesCLTLS,TsinghuaUniversit

Immunological techniques • Immunological techniques include: • experimental methods to study the immune system • methods to generate or use immunological reagents as experimental tools. • The most common immunological methods : production and use of antibodies to detect specific proteins in biological samples. • http://www.nature.com/subjects/immunological-techniques CLTLS, Tsinghua University

Immunochemical techniguesand.Immunochemistry:studyofbiochemicalmolecular aspects of immunology, especially thenature of antibodies, antigens and their interactionshttp://goldbook.iupac.org/l02980.html?The:ofantibodies(andotherstudysomesuchmoleculesimmunologicallyasimportantisknowncomplementascomponentsSuchmethodsareknownimmunochemistryasimmunochemicaltechniques.CLTLS,TsinghuaUniversit

Immunochemical techniques • Immunochemistry: study of biochemical and molecular aspects of immunology, especially the nature of antibodies, antigens and their interactions. http://goldbook.iupac.org/I02980.html • The study of antibodies (and some other immunologically important molecules such as complement components) is known as immunochemistry. Such methods are known as immunochemical techniques. CLTLS, Tsinghua University

Antigen & AntibodyCLTLS,Tsinghua Universit

Antigen & Antibody CLTLS, Tsinghua University

Antigen·Anantigen(Ag)isasubstanceImmunogenicity/antigenicity-capableof causinganimmuneresponseleadingtotheproductionofantibodiesImmunoreactivity/reactogenicity-targeted byantibodiesCLTLS,TsinghuaUniversit

Antigen • An antigen (Ag) is a substance • Immunogenicity/antigenicity-capable of causing an immune response leading to the production of antibodies • Immunoreactivity/reactogenicity-targeted by antibodies. CLTLS, Tsinghua University