扬州大学：《组织胚胎学》课程授课教案 MBSS teaching plan

TeachingPlanforBachelor of Medicine and Bachelor of Surgery (MBSS)Department of Histology, School of Medicine, Yangzou UniversityCourseHistology&EmbryologyTextbookTheTextbook of Histologyand Embryology (Yingmao Gao)Teaching TypeLectureRealia(1) PPT(2)OnlinePlatform(http://zzptx.yzuyxy.cn/en/)Reference BooksI. Junqueira's Basic Histology: Text and Atlas, 13e (Anthony Mescher)2. Langman's Medical Embryology (Thomas W. Sadler)Unit1 Introduction to HistologyClass Hours2Objectives1.TOMASTER(1) The definition of Histology;(2) The definition of Tissue and Four Basic Tissues;(3) Hematoxylin and Eosin (H.E) staining method;(4)The definition of basophilia, acidophilia, argentaffin and argyrophilia(5) The definition of ultrastructure;(6)PAS Reaction

Teaching Plan for Bachelor of Medicine and Bachelor of Surgery (MBSS) Department of Histology, School of Medicine, Yangzou University Course Histology & Embryology Textbook The Textbook of Histology and Embryology (Yingmao Gao) Teaching Type Lecture Realia （1）PPT （2）Online Platform（http://zzptx.yzuyxy.cn/en/） Reference Books 1. Junqueira's Basic Histology: Text and Atlas, 13e (Anthony Mescher) 2. Langman's Medical Embryology (Thomas W. Sadler) Unit 1 Introduction to Histology Class Hours 2 Objectives 1. TO MASTER (1) The definition of Histology; (2) The definition of Tissue and Four Basic Tissues; (3) Hematoxylin and Eosin (H.E) staining method; (4) The definition of basophilia, acidophilia, argentaffin and argyrophilia; (5) The definition of ultrastructure; (6) PAS Reaction

2.TOUNDERSTAND(1) The procedure of Paraffin Sectioning;(2) The difference between Transmission EM and Scanning EM;(3)Immunohistochemistry,in situhybridizationandcell cultureKeyPoints1.The definition of Tissue and Four Basic Tissues:2. Hematoxylin and Eosin (H.E) staining method;3.The definition of basophilia,acidophilia, argentaffin and argyrophilia;4. The difference between Transmission EM and Scanning EM;5.PAS ReactionDifficult Points1.HematoxylinandEosin (H.E)stainingmethod2. The definition of basophilia and acidophilia,3. The difference between Transmission EM and Scanning EM;4.PAS Reaction.Teaching contents (90 min)1.Definition and contents of Histology (10 min)2.Relationship of Histology to other medical courses (10 min)3. Studying method (70 min)a.Lightmicroscopy:Paraffin Sectioning andH&E staining(25min)b.Electron microscopy (20 min)c. Histochemistry and cytochemistry: PAS reaction (10 min)d. Immunocytochemistry (5 min)e. In situ hybridization (5 min)f. Cell culture (5 min)AssignmentAnswering Questions1. What is the H&E staining?2.What are the differences between the light and electron microscopy?3.Howdoesahistologist studythe livingcellsandtissues?

2. TO UNDERSTAND (1) The procedure of Paraffin Sectioning; (2) The difference between Transmission EM and Scanning EM; (3) Immunohistochemistry, in situ hybridization and cell culture. Key Points 1. The definition of Tissue and Four Basic Tissues; 2. Hematoxylin and Eosin (H.E) staining method; 3. The definition of basophilia, acidophilia, argentaffin and argyrophilia; 4. The difference between Transmission EM and Scanning EM; 5. PAS Reaction. Difficult Points 1. Hematoxylin and Eosin (H.E) staining method; 2. The definition of basophilia and acidophilia; 3. The difference between Transmission EM and Scanning EM; 4. PAS Reaction. Teaching contents (90 min) 1. Definition and contents of Histology (10 min) 2. Relationship of Histology to other medical courses (10 min) 3. Studying method (70 min) a. Light microscopy: Paraffin Sectioning and H&E staining (25 min) b. Electron microscopy (20 min) c. Histochemistry and cytochemistry: PAS reaction (10 min) d. Immunocytochemistry (5 min) e. In situ hybridization (5 min) f. Cell culture (5 min) Assignment Answering Questions: 1. What is the H&E staining? 2.What are the differences between the light and electron microscopy? 3. How does a histologist study the living cells and tissues?

Unit2EpithelialTissueClassHours4Objectives1.TOMASTER(1) The structural characteristics, distribution and function of the epithelium(2) The structure,function and distribution of simple squamous epithelium(including endothelium and mesothelium, simple cuboidal epithelium, simplecolumnar epithelium, pseudostratified columnar ciliated epithelium, stratifiedsquamous epithelium and transitional epithelium);(3)Thespecializations oftheepithelium:thespecializationsinfree surface(ciliaand microvilli), lateral surface (junctional complex)and basal surface (basementmembrane),(4) Concept of the glandular epithelium and gland;(5) Structural characteristics of the endocrine gland and exocrine gland2.TOUNDERSTAND(l) intercellular junctions (tight junction, desmosome, intermediate junction andgap junction);(2) The regeneration of the epithelium.KeyPoints1. The structural characteristics, distribution and function of the epithelium;2. The structure, function and distribution of simple squamous epithelium(includingendotheliumandmesothelium,simplecuboidalepithelium,simplecolumnar epithelium, pseudostratified columnar ciliated epithelium, stratifiedsquamous epithelium and transitional epithelium);3.The specializations of the epithelium:the specializations in free surface (ciliaand microvilli), lateral surface (junctional complex) and basal surface (basementmembrane);4. Concept of the glandular epithelium and gland;

Unit 2 Epithelial Tissue Class Hours 4 Objectives 1. TO MASTER (1) The structural characteristics, distribution and function of the epithelium; (2) The structure, function and distribution of simple squamous epithelium (including endothelium and mesothelium, simple cuboidal epithelium, simple columnar epithelium, pseudostratified columnar ciliated epithelium, stratified squamous epithelium and transitional epithelium); (3) The specializations of the epithelium: the specializations in free surface (cilia and microvilli), lateral surface (junctional complex) and basal surface (basement membrane); (4) Concept of the glandular epithelium and gland; (5) Structural characteristics of the endocrine gland and exocrine gland. 2. TO UNDERSTAND (1) intercellular junctions (tight junction, desmosome, intermediate junction and gap junction); (2) The regeneration of the epithelium. Key Points 1. The structural characteristics, distribution and function of the epithelium; 2. The structure, function and distribution of simple squamous epithelium (including endothelium and mesothelium, simple cuboidal epithelium, simple columnar epithelium, pseudostratified columnar ciliated epithelium, stratified squamous epithelium and transitional epithelium); 3. The specializations of the epithelium: the specializations in free surface (cilia and microvilli), lateral surface (junctional complex) and basal surface (basement membrane); 4. Concept of the glandular epithelium and gland;

5. Structural characteristics of the endocrine gland and exocrine glandDifficultPoints1. The structure, function and distribution of pseudostratified columnar ciliatedepithelium and transitional epithelium;2. Cilia and microvilli;3.Tight junction, desmosome, intermediate junction and gap junction;4. Structural characteristics of Serous gland cell and mucous gland cellTeaching contents (180 min)1. Classification and general features of the epithelial tissues (20 min)2. Covering epithelia: type, distribution, and function (50 min)3.Epithelial specializations (60 min)a. Free surface: microvilli, cilia (15 min)b.Lateral surface: cell junctions (25 min)c.Basal surface: basement membrane, plasma membrane infolding,hemidesmosome(20 min)4. Glandular epithelia and glands (40 min)5.Renewal of epithelial tissue (10min)AssignmentExplanation of Terms1.Microvilli2.Cilia3. Gap junction4.Basement membraneAnswering Questions1.Explainthe structural characteristics andbasis of classification of epithelium2.Comparemicrovillus with ciliumUnit3ConnectiveTissueClass Hours3

5. Structural characteristics of the endocrine gland and exocrine gland. Difficult Points 1. The structure, function and distribution of pseudostratified columnar ciliated epithelium and transitional epithelium; 2. Cilia and microvilli; 3. Tight junction, desmosome, intermediate junction and gap junction; 4. Structural characteristics of Serous gland cell and mucous gland cell. Teaching contents (180 min) 1. Classification and general features of the epithelial tissues (20 min) 2. Covering epithelia: type, distribution, and function (50 min) 3. Epithelial specializations (60 min) a. Free surface: microvilli, cilia (15 min) b. Lateral surface: cell junctions (25 min) c. Basal surface: basement membrane, plasma membrane infolding, hemidesmosome (20 min) 4. Glandular epithelia and glands (40 min) 5. Renewal of epithelial tissue (10 min) Assignment Explanation of Terms 1.Microvilli 2. Cilia 3. Gap junction 4. Basement membrane Answering Questions 1. Explain the structural characteristics and basis of classification of epithelium. 2. Compare microvillus with cilium. Unit 3 Connective Tissue Class Hours 3

Objectives1.TOMASTER(1) The structural characteristics and classification of the connective tissue;(2) The structural characteristic and function of loose connective tissue;(3) The chemical constituents, morphological structure and function of 3 fiber types(collagenous, elastic and reticular fibers),(4) The chemical constituents and characteristics of the matrix of intercellularsubstance;(5) The structure and function of several main cell types in connective tissue(fibroblast, macrophage, plasma cell, mast cell, fat cell and mesenchymal cell)2.TOUNDERSTAND(1) The structural characteristics and function of dense connective tissue, adiposetissue, reticular tissue and the tissue fluid.KeyPoints1. The structural characteristics and classification of the connective tissue;2. The structure and function of several main cell types in connective tissue(fibroblast, macrophage, plasma cell, mast cell, fat cell and mesenchymal cell),3.The chemical constituents, morphological structure and function of three fibertypes (collagenous, elastic and reticular fibers);4. The chemical constituents and characteristics of the matrix of intercellularsubstanceDifficult Points1.The structure and function of several main cell types in connective tissue(fibroblast, macrophage, plasma cell, mast cell, fat cell and mesenchymal cell):2. The chemical constituents, morphological structure and function of three fibertypes (collagenous, elastic and reticularfibers);3. The chemical constituents and characteristics of the matrix of intercellularsubstanceTeaching contents (135 min)1. Classification and general characteristics of connective tissue (20 min)

Objectives 1. TO MASTER (1) The structural characteristics and classification of the connective tissue; (2) The structural characteristic and function of loose connective tissue; (3) The chemical constituents, morphological structure and function of 3 fiber types (collagenous, elastic and reticular fibers); (4) The chemical constituents and characteristics of the matrix of intercellular substance; (5) The structure and function of several main cell types in connective tissue (fibroblast, macrophage, plasma cell, mast cell, fat cell and mesenchymal cell). 2. TO UNDERSTAND (1) The structural characteristics and function of dense connective tissue, adipose tissue, reticular tissue and the tissue fluid. Key Points 1. The structural characteristics and classification of the connective tissue; 2. The structure and function of several main cell types in connective tissue (fibroblast, macrophage, plasma cell, mast cell, fat cell and mesenchymal cell); 3. The chemical constituents, morphological structure and function of three fiber types (collagenous, elastic and reticular fibers); 4. The chemical constituents and characteristics of the matrix of intercellular substance. Difficult Points 1. The structure and function of several main cell types in connective tissue (fibroblast, macrophage, plasma cell, mast cell, fat cell and mesenchymal cell); 2. The chemical constituents, morphological structure and function of three fiber types (collagenous, elastic and reticular fibers); 3. The chemical constituents and characteristics of the matrix of intercellular substance. Teaching contents (135 min) 1. Classification and general characteristics of connective tissue (20 min)

2.Looseconnectivetissue(90min)a. Cells: structures and functions of fibroblasts, macrophages, plasma cells, mastcells, fat cells, mesenchymal cells and leukocytes (50 min)b. Fibers: morphology and function of collagenous fibers, elastic fibers, andreticular fibers (20 min)c. Ground substance: chemical composition, molecular sieve (20 min)3. Dense connective tissue, adipose tissue, and reticular tissue (25 min)AssignmentExplanation of Terms1.Molecular sieves2. Tissue fluidAnswering questions1. How many types of tissues dose the connective tissue include?2. What components dose the loose connective tissue contain?3. Describe the structure of fibroblast, macrophage, plasma cell and mast cell4. Taking the degranulation of mast cell as an example describe relationshipbetween the structure and function.Unit 4 Cartilage and boneClass Hours3Objectives1. TO MASTER() The structural feature and classification of cartilage;(2) The composition and structure of the osseous tissue;(3) The structure of cancellous (spongy) bone and compact bone;(4) The structure and function of several main cell types in connective tissue(fibroblast, macrophage, plasma cell, mast cell, fat cell and mesenchymal cell)2.TOUNDERSTAND(1) The generating condition of the bone formation;

2. Loose connective tissue (90 min) a. Cells: structures and functions of fibroblasts, macrophages, plasma cells, mast cells; fat cells, mesenchymal cells and leukocytes (50 min) b. Fibers: morphology and function of collagenous fibers, elastic fibers, and reticular fibers (20 min) c. Ground substance: chemical composition, molecular sieve (20 min) 3. Dense connective tissue, adipose tissue, and reticular tissue (25 min) Assignment Explanation of Terms 1. Molecular sieves 2. Tissue fluid Answering questions 1. How many types of tissues dose the connective tissue include? 2. What components dose the loose connective tissue contain? 3. Describe the structure of fibroblast, macrophage, plasma cell and mast cell. 4. Taking the degranulation of mast cell as an example describe relationship between the structure and function. Unit 4 Cartilage and bone Class Hours 3 Objectives 1. TO MASTER (1) The structural feature and classification of cartilage; (2) The composition and structure of the osseous tissue; (3) The structure of cancellous (spongy) bone and compact bone; (4) The structure and function of several main cell types in connective tissue (fibroblast, macrophage, plasma cell, mast cell, fat cell and mesenchymal cell). 2. TO UNDERSTAND (1) The generating condition of the bone formation;

(2) Two modes of osteogenesis (intramembranous ossification and endochondralossification);(3) The structure and function of the periosteum and endosteumKeyPoints1. The structural feature and classification of cartilage;2. The composition and structure of the osseous tissue;3.The structureof compact boneDifficultPoints1. The structural feature and classification of cartilage;2. The composition and structure of the osseous tissue;3.The structure of compact bone;4 Two modes of osteogenesis (intramembranous ossification and endochondralossification).Teaching contents (135 min)1.Classification and general characteristics of cartilage (20 min)2. Hyaline cartilage: structure and distribution, perichondrium and growth. (20 min)3. Elastic cartilage and fibrocartilage (20 min)4. Structure of the bone tissue: cells (osteoblasts,osteocytes, osteoclasts), bonelamellae. (50 min)5. Structure of the long bone: compact bone (circumferential lamellae, osteons,interstitial lamellae),spongy bone,periosteum and endosteum; bone formation.(25min)AssignmentExplanation of Terms1.Isogenous group2.OsteonAnswering questions1.What components dose the cartilagematrix consist of?2. Describe the structural features of three types of cartilage3.Describe the main characteristics ofthe cells in the osseous tissue

(2) Two modes of osteogenesis (intramembranous ossification and endochondral ossification); (3) The structure and function of the periosteum and endosteum. Key Points 1. The structural feature and classification of cartilage; 2. The composition and structure of the osseous tissue; 3. The structure of compact bone Difficult Points 1. The structural feature and classification of cartilage; 2. The composition and structure of the osseous tissue; 3. The structure of compact bone; 4 Two modes of osteogenesis (intramembranous ossification and endochondral ossification). Teaching contents (135 min) 1. Classification and general characteristics of cartilage (20 min) 2. Hyaline cartilage: structure and distribution, perichondrium and growth. (20 min) 3. Elastic cartilage and fibrocartilage (20 min) 4. Structure of the bone tissue: cells (osteoblasts, osteocytes, osteoclasts), bone lamellae. (50 min) 5. Structure of the long bone: compact bone (circumferential lamellae, osteons, interstitial lamellae), spongy bone, periosteum and endosteum; bone formation. (25min) Assignment Explanation of Terms 1.Isogenous group 2.Osteon Answering questions 1. What components dose the cartilage matrix consist of? 2. Describe the structural features of three types of cartilage. 3. Describe the main characteristics of the cells in the osseous tissue

4. How many kinds of bone lamellae are there in compact bone of a long bone?Unit 5 BloodClass Hours3Objectives1.TOMASTER(1) The composition and classification of the blood;(2) The form, size, structure and normal value of red blood cell;(3) The characteristics of morphology and normal value of reticulocyte;(4) The classification and normal value of white blood cell;(5) The morphologic structure and function of granulocyte types;(6) Themorphologic structure and function of lymphocyte;(7) The morphologic structure and function of monocyte;(8) The morphologic structure, function and normal value of platelets2.TOUNDERSTAND(1) The normal value and clinical significance of each series of white blood cell;(2) Histological structure and function of red bone marrow,(3) Rules of morphologic changes of blood cells and platelet in hemopoiesis.KeyPoints1. The structure and function of the formed elements of blood2. The relationship between structure and function of the blood cellsDifficult Points1. The structure and function of the formed elements of blood;2. The relationship between structure and function of the blood cells;3. Rules of morphologic changes of blood cells and platelet in hemopoiesisTeachingcontents (135min)1. The composition of the blood (20 min)(1) Conception of plasma, serum and the formed elements of blood. (10 min)(2)Theclassificationandnormalvalueoftheformedelements.(10min)

4. How many kinds of bone lamellae are there in compact bone of a long bone? Unit 5 Blood Class Hours 3 Objectives 1. TO MASTER (1) The composition and classification of the blood; (2) The form, size, structure and normal value of red blood cell; (3) The characteristics of morphology and normal value of reticulocyte; (4) The classification and normal value of white blood cell; (5) The morphologic structure and function of granulocyte types; (6) The morphologic structure and function of lymphocyte; (7) The morphologic structure and function of monocyte; (8) The morphologic structure, function and normal value of platelets. 2. TO UNDERSTAND (1) The normal value and clinical significance of each series of white blood cell; (2) Histological structure and function of red bone marrow; (3) Rules of morphologic changes of blood cells and platelet in hemopoiesis. Key Points 1. The structure and function of the formed elements of blood; 2. The relationship between structure and function of the blood cells. Difficult Points 1. The structure and function of the formed elements of blood; 2. The relationship between structure and function of the blood cells; 3. Rules of morphologic changes of blood cells and platelet in hemopoiesis. Teaching contents (135 min) 1. The composition of the blood (20 min) (1) Conception of plasma, serum and the formed elements of blood. (10 min) (2) The classification and normal value of the formed elements. (10 min)

2.Themorphologic and structural characteristicsof blood cells (90min)Red blood cell (30 min)(1) The form, size and structure of red blood cell (10 min)(2) The normal value and function of Hb (15 min)(3)Thecharacteristics ofmorphology and normal value of reticulocyte(5min)White blood cell (60 min)(1) The classification and normal value of white blood cell (10 min)(2) The morphologic structure and function of granulocyte types (30 min)(3) Lymphocyte and monocyte (20 min)3. Platelets (10 min)The morphologic structure, function and normal value of platelets4. The development of blood cells (25 min)(1) Hemopoietic stem cell and hemopoietic organ (5 min)(2)Rules of morphologic changes of blood cells and platelet in hemopoiesis(10 min)AssignmentExplanation of Terms1.ReticulocyteAnswering questions1.Descript the morphologic structure and function of erythrocytes2. Compare the morphologic structure and function of granulocytes (includeneutrophils,eosinophilsandbasophils)3.Compare lymphocyte with monocyte in the structure and function4.Answer theorigin,migration and biologic feature of hematopoietic stem cell5. Descript the general law of morphologic change of hematopoietic cell in thedevelopment of blood cellUnit6Muscle TissueClass Hours

2. The morphologic and structural characteristics of blood cells (90 min) Red blood cell (30 min) (1) The form, size and structure of red blood cell (10 min) (2) The normal value and function of Hb (15 min) (3) The characteristics of morphology and normal value of reticulocyte (5min) White blood cell (60 min) (1) The classification and normal value of white blood cell (10 min) (2) The morphologic structure and function of granulocyte types (30 min) (3) Lymphocyte and monocyte (20 min) 3. Platelets (10 min) The morphologic structure, function and normal value of platelets. 4. The development of blood cells (25 min) (1) Hemopoietic stem cell and hemopoietic organ (5 min) (2) Rules of morphologic changes of blood cells and platelet in hemopoiesis (10 min) Assignment Explanation of Terms 1. Reticulocyte Answering questions 1. Descript the morphologic structure and function of erythrocytes. 2. Compare the morphologic structure and function of granulocytes (include neutrophils, eosinophils and basophils). 3. Compare lymphocyte with monocyte in the structure and function. 4. Answer the origin, migration and biologic feature of hematopoietic stem cell. 5. Descript the general law of morphologic change of hematopoietic cell in the development of blood cell. Unit 6 Muscle Tissue Class Hours

3Objectives1.TOMASTER(1) The structural characteristic, function and classification of muscle tissue;(2) The form and structure of skeletal muscle fiber, striation and sarcomere;(3) the structural characteristic of myofibril (types of myofilament), transversetubule and sarcoplasmic reticulum and their relationship with the musclecontraction,(4) The structural characteristic of cardiac and the intercalated discs2.TOUNDERSTAND(1) The molecular structure and contraction mechanism of skeletal muscle;(2) the structural characteristics of smooth muscle.KeyPoints1. The structural characteristic, function and classification of muscle tissue,2. The form and structure of skeletal muscle fiber, striation and sarcomere;3. The structural characteristic of cardiac and the intercalated discs;4. The structural characteristic of myofibril (types of myofilament), transverse tubuleand sarcoplasmic reticulum and their relationship with themuscle contraction.Difficult Points1. The form and structure of skeletal muscle fiber, striation and sarcomere;2. The structural characteristic of cardiac and the intercalated discs;3. The structural characteristic of myofibril (types of myofilament), transverse tubuleand sarcoplasmic reticulum and theirrelationshipwith the musclecontractionTeaching contents (135 min)1. General features and classification of the muscular tissue (15 min)2. Skeletal muscle (60 min)a. LM structure: shape, nucleus, cross striation, myofibril (20 min)b. EM structure: myofibril, sarcomere, transverse tubule, sarcoplasmic reticulum,triad (20 min)c. Mechanism of contraction (sliding filament hypothesis) (20 min)

3 Objectives 1. TO MASTER (1) The structural characteristic, function and classification of muscle tissue; (2) The form and structure of skeletal muscle fiber, striation and sarcomere; (3) the structural characteristic of myofibril (types of myofilament), transverse tubule and sarcoplasmic reticulum and their relationship with the muscle contraction; (4) The structural characteristic of cardiac and the intercalated discs. 2. TO UNDERSTAND (1) The molecular structure and contraction mechanism of skeletal muscle; (2) the structural characteristics of smooth muscle. Key Points 1. The structural characteristic, function and classification of muscle tissue; 2. The form and structure of skeletal muscle fiber, striation and sarcomere; 3. The structural characteristic of cardiac and the intercalated discs; 4. The structural characteristic of myofibril (types of myofilament), transverse tubule and sarcoplasmic reticulum and their relationship with the muscle contraction. Difficult Points 1. The form and structure of skeletal muscle fiber, striation and sarcomere; 2. The structural characteristic of cardiac and the intercalated discs; 3. The structural characteristic of myofibril (types of myofilament), transverse tubule and sarcoplasmic reticulum and their relationship with the muscle contraction. Teaching contents (135 min) 1. General features and classification of the muscular tissue (15 min) 2. Skeletal muscle (60 min) a. LM structure: shape, nucleus, cross striation, myofibril (20 min) b. EM structure: myofibril, sarcomere, transverse tubule, sarcoplasmic reticulum, triad (20 min) c. Mechanism of contraction (sliding filament hypothesis) (20 min)