复旦大学：《医学微生物学 Medical Microbiology》学生讨论课课件_流感嗜血杆菌 Haemophilus influenzae

Haemophilus influenzae 流感嗜血杆菌 Medical Microbiology 小组:黄锃恕、刘师学、章哲宇

Haemophilus influenzae 流感嗜血杆菌 小组：黄锃恕、刘师学、章哲宇 Medical Microbiology

Haemophilus influenzae 1pm×0.3m gram-negative rods or coccobacilli PART 1 non-motile Biological non-spore-forming characteristics catalase: positive oxidase: positive facultatively Haemophilus infiuenzae anaerobic In cerebrospinal fluid some ot the cell may be elongated to several times their usual length(pleomorphic Medical Microbiology

Haemophilus influenzae 1 µm × 0.3 µm gram-negative rods or coccobacilli non-motile non-spore-forming catalase: positive oxidase: positive facultatively anaerobic In cerebrospinal fluid some of the cell may be elongated to several times their usual length (pleomorphic). PART 1 Biological characteristics Medical Microbiology

Culture of Haemophilus influenzae www.microbiologyinpictures.com PART 1 Biological X+V factor characteristics Xfao→ V facto Haemophilus influenzae cultivation chocolate agar 24hous,37℃,5%CO2 Medical Microbiology

Culture of Haemophilus influenzae PART 1 Biological characteristics Medical Microbiology

Culture of Haemophilus influenzae Requirement of X+v factor in cultivation media X= hematin, V= nicotinamide adenine dinucleotide(nad) PART 1 Growth on chocolate agar but not on blood agar Biological (when cultivated in pure culture characteristics Satelite phenomenon with Staphylococcus aureus (on blood agar) Medical Microbiology

Requirement of X+V factor in cultivation media X = hematin, V = nicotinamide adenine dinucleotide(NAD) Growth on chocolate agar but not on blood agar (when cultivated in pure culture) Satelite phenomenon with Staphylococcus aureus (on blood agar) Culture of Haemophilus influenzae PART 1 Biological characteristics Medical Microbiology

Culture of Haemophilus influenzae wamicrobiologyinpictures.com PART 1 Biological characteristics Staphylococcus aureus Haemophilus influenzae an area without visible growth Medical The satellite test Microbiology

Culture of Haemophilus influenzae PART 1 Biological characteristics Medical Microbiology

Biochemical reaction Glucose Sucrose PART 1 Biological Lactose D-Mannitol characteristics Galactose\ Fructose Maltose Medical Microbiology

Biochemical reaction PART 1 Biological characteristics Medical Microbiology Glucose + Sucrose + Lactose - D-Mannitol - Galactose\Fructose\Maltose ±

Classification 6 different types(a-f) Nonencapsulated PART 1 The most virulent strain H influenzae Biological iS H influenzae type b. Nontypeable characteristics (Hik H influenzae(NTHi A polysaccharide ∵:1 capsule No capsule 心,: Medical Microbiology

• Nonencapsulated H. influenzae • Nontypeable H. influenzae (NTHi) No capsule • 6 different types (a-f) • The most virulent strain is H. influenzae type b (Hib). A polysaccharide capsule Classification PART 1 Biological characteristics Medical Microbiology

Hib: H influenzae type b Pathogenic substances capsule PART 2 Pathogencity Anti phagocytic effect And Main virulence factor Immunity Polyriboseribitol phosphate, PRP lgA protease Hydrolyze IgA Medical Microbiology

Capsule • Anti phagocytic effect • Main virulence factor • Polyriboseribitol phosphate, PRP IgA protease • Hydrolyze IgA Hib: H. influenzae type b Pathogenic substances PART 2 Pathogenicity And Immunity Medical Microbiology

Hib: H influenzae type b Diseases The most virulent strain Accounts for more than 95% of H influenzae invasive diseases in children and half of invasive diseases in PART 2 Pathogencity aduLts And Less-common Immunity Common diseases diseases · Bacteremia Endophthalmitis Meningitis Urinary tract intection Cellulitis Abscesses Epiglottitis · Cervical adenitis Septic arthritis Osteomyelitis Pneumonia · Endocarditis Medical Empyema Microbiology

The most virulent strain Accounts for more than 95% of H. influenzae invasive diseases in children and half of invasive diseases in adults. Common diseases • Bacteremia • Meningitis • Cellulitis • Epiglottitis • Septic arthritis • Pneumonia • Empyema Less-common diseases • Endophthalmitis • Urinary tract infection • Abscesses • Cervical adenitis • Osteomyelitis • Endocarditis Hib: H. influenzae type b Diseases PART 2 Pathogenicity And Immunity Medical Microbiology

Hib: H influenzae type b Immunology Host defenses include the activation of the alternative and classical complement pathways and antibodies to the prp capsule PART 2 The antibody to the Hib capsule plays the primary role Pathogencity in conferring immunity And Newborns have a low risk of infection Immunity 抗原抗体复合物(G或oM)「经典途径 激活的C1 ML途径 C4+c2 CAb2 C4b2b3b (C3转化酶) 微活 (C5转化酶 病原体甘露糖残基 C3b C5b c5b-9 攻膜复合物) c6 c7 c8 c0 C3-. C3b 7↑c C3bBb C3bBb3b C3转化酶) (c5转化酶) Medical B因子D因子 Microbiology 旁路途径

Host defenses include the activation of the alternative and classical complement pathways and antibodies to the PRP capsule. The antibody to the Hib capsule plays the primary role in conferring immunity. Newborns have a low risk of infection. Hib: H. influenzae type b Immunology PART 2 Pathogenicity And Immunity Medical Microbiology