北京大学：《微生物学》课程PPT教学课件（英文版）Industrial Microbiology and Fermentation Technology

Industrial Microbiology and Fermentation Technology Textbook: Biology of Microorganisms Seventh edition By Thomas D. Brock et al. (1996) Websitehttp://www.prenhall.com/brock Lecturer: CHEN GUO- Qiang(陈国强) microbes(@public3bta.net.cn Tel: 62783844. Room 102. School of Life sciences

Industrial Microbiology and Fermentation Technology Textbook: Biology of Microorganisms Seventh Edition By Thomas D. Brock et al. (1996) Website: http://www.prenhall.com/~brock Lecturer: CHEN Guo-Qiang (陈国强) microbes@public3.bta.net.cn Tel: 62783844, Room 102, School of Life Sciences

Industrial microbiology and Fermentation Technology Industrial Microbiology is the discipline that uses microorganisms, usually grown on a large scale to produce valuable commercial products or carry out important chemical transformations Fermentation Technology is the technology to grow cells in a large scale with high efficiency, it also includes product recovery processes

Industrial Microbiology and Fermentation Technology • Industrial Microbiology is the discipline that uses microorganisms, usually grown on a large scale, to produce valuable commercial products or carry out important chemical transformations. • Fermentation Technology is the technology to grow cells in a large scale with high efficiency, it also includes product recovery processes

Microbial products: microbial cells, enzymes, pharmaceutical products Specialty chemicals and food additives Cells Bioconversion Products from cells Substrate 00 Enzymes (for example (for example glucose 08.880>cells citic acid) Isomerase Antibiotics Alcohol (for exampl (ethanol) Product Yeast Food cells (for example, additives steroid bioconversions amino acos

Microbial products: microbial cells, enzymes, pharmaceutical products Specialty Chemicals and food additives

Flowsheet for developing an industrial microbial fermentation process Strain selection Laboratory process development Pilot Scale up Industrial Scale up Downstream process development Product packaging techniques Other commercial consideration Examples

Flowsheet for developing an industrial microbial fermentation process • Strain selection • Laboratory process development • Pilot Scale up • Industrial Scale up • Downstream process development • Product packaging techniques • Other commercial consideration • Examples

Strain selection Purchase from Culture collections -Screening of nature circumstances enetic engineering Mutations Cell biology techniques

Strain Selection –Purchase from Culture Collections –Screening of nature circumstances –Genetic engineering –Mutations –Cell biology techniques

International Culture collections TABLE 12.1 Culture collections that supply cultures of industrial microorganisms" Abbreviation Name Location ATCC American Type Culture Collection Rockville, MD, United States Centraalbureau voor Schimmelculturen aarn. The Netherlands CCM Czechoslovak Collection of Microorganisms J. E Purkyne University, Brno, Czech Republic CDDA Canadian Department of Agriculture Ottawa, Canada CMI Commonwealth Mycological Institute Kew, United Kingdom Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH Braunschweig, Germany FAT Faculty of Agriculture, Tokyo University Tokyo, Japan IAM Institute of Applied Microbiology University of Tokyo, Japan NCIB National Collection of Industrial Bacteria Aberdeen scotland NCTC National Collection of Type Cultures London, United Kingdom Northern Regional Research Laboratory Peoria, IL, United States PCC Pasteur Culture Collection Paris, france "Listed here are just a few of the general culture collections. Many universities and research laboratories maintain collections of specific microbial groups. Domestically, strains can be purchased from: CGMCC or China general microbiological culture collection center 中国微生物菌种保藏管理委员会普通微生物中心,北京中关村

International Culture Collections Domestically, strains can be purchased from: CGMCC or China General Microbiological Culture Collection Center 中国微生物菌种保藏管理委员会普通微生物中心，北京中关村

Screening of nature circumstances

Screening of nature circumstances

BamHI site Genetic BamHI sites Engineering BamHI Foreign DNA Incorporation into BamHI artificial plasmids of genes from a wide variety of sources has made possible the DNA ligase transfer of genetic material across virtually any species barrier Hybrid containing Recycled foreign DNA Various high value DBR322 added products have Transformation of E col been produced from Transformants Transformants the genetic engineering resistant to both resistant to ampicillin ampicillin and tetracycline but sensitive to tetracycline methoas

Genetic Engineering Incorporation into artificial plasmids of genes from a wide variety of sources has made possible the transfer of genetic material across virtually any species barrier Various high value added products have been produced from the Genetic engineering methods

Mutation Via chemica or physical, and biological means

Mutation Via chemical or physical, and biological means

Cell biology techniques Protoplast fusion(promote high frequencies of genetic recombinants) removing the cell wall with lytic enzymes in the presence of osmotic stabilizers. In the presence of fusogenic agent such as polyethylene glycol (eg), protoplasts are induced to fuse and form transient hybrids or diploids Regeneration of viable cells from the fused protoplasts

Cell biology techniques • Protoplast fusion (promote high frequencies of genetic recombinants) – removing the cell wall with lytic enzymes in the presence of osmotic stabilizers. – In the presence of fusogenic agent such as polyethylene glycol (PEG), protoplasts are induced to fuse and form transient hybrids or diploids. – Regeneration of viable cells from the fused protoplasts