北京大学：《微生物学》课程PPT教学课件（英文版）Cell Biology Tool for studying microbiology

Cell biolog sk s Tool for studying microbiology Microscopes and Microscopy Microscope (a) bright-field (b) Phase contrast (c) Dark-field (d) Fluorescence

Cell Biology • Tool for studying microbiology: Microscopes and Microscopy Microscope: (a) Bright-field (b) Phase contrast (c) Dark-field (d) Fluorescence

Staining Dves can be used to stain cells and increase their contrast- Gram Staining Flood the heat.tb ar with crystal voiet for 1 rm Al cans r purpl ass sade throug o toto about 20 sec Gramp cais are examine with 1oo objective

Staining: Dyes can be used to stain cells and increase their contrast-Gram Staining

The Prokaryotic Cells ANNHAIC W CAYNPAICSAMNHR W AYNPCAyY Consisting of cell wall, cytoplasmic membrane, ribosomes inclusions, chromosome, flagella cel wnl Cytopl ano Mitochondna Cyton

The Prokaryotic Cells Consisting of cell wall, cytoplasmic membrane, ribosomes, inclusions, chromosome, flagella

Morphology of Prokaryotes Coccus or Cocci Rod Spirillum or spirilla Spirochete Budding and appendaged A Filamentous

Morphology of Prokaryotes Coccus or Cocci Rod Spirillum or Spirilla Spirochete Budding and appendaged Filamentous

The Eukaryotic Cells Cell wall Cytoplasmic membran Chloroplast 密 Mitocnonono ∵

The Eukaryotic Cells Chloroplast

Cytoplasmic Membrane: Structure H2o Glycerol Ho H2o FIGURE 3.13 (a)Fundamental structure of phospholipid bilayer. (b) Phospholipid membrane vesicle-a stable arrangement of lipids in

Cytoplasmic Membrane: Structure

Cytoplasmic Membrane: Structure Lipid bilayers, flexible structures, embedded with proteins

Cytoplasmic Membrane: Structure • Lipid bilayers, flexible structures, embedded with proteins

Structure of the cytoplasmic membrane Hydrope Prokaryotes Membrane stabilizers Phospholipid hydrogen bonds molecule hydrophobic interactions Mg, Caz+, hopanoids

Structure of the cytoplasmic membrane Prokaryotes Membrane Stabilizers: hydrogen bonds hydrophobic interactions Mg2+, Ca2+, hopanoids

D Membrane Streng thening Agents. Sterols and Hopanoids 3 (a) general structure of a sterol HC-CH (b) The structure of cholesterol CH2 (c) The structure of the hopanoid H2 HC-CH diploptene D Enkaryotes membrane stabilizer Sterol Hydrophobic CH A B c CH3

Membrane Strengthening Agents: Sterols and Hopanoids (a) General structure of a sterol; (b) The structure of cholesterol; (c) The structure of the hopanoid diploptene. Enkaryotes membrane stabilizer: Sterol

Archaeal membranes Chemical Inkages in lipids (a)ester linkage H2C-0-CFR (b)ether linkage H2C-0-C-R (c)isoprene C-0-CFR HC-0-C-R 0 0 H2C-0-P-0H2c-0-P-0 H2C=C-C=, 0 0

Archaeal Membranes Chemical linkages in lipids: (a) ester linkage (b) ether linkage (c) isoprene