上海交通大学：《医学实验技术》课程教学课件（PPT讲稿）激光扫描共聚焦技术

激光扫描共聚焦技术

激光扫描共聚焦技术

Basics Fluorescence Imaging荧光成像基础

Excitation and emission of fluorescent molecule荧光分子的激发与发射Recovered恢复Resting稳态Excited激发态excitationlight激发光emitedheat&fluorescence发热j炎水b1.00.5SAO600500400Wavelength波长(nm）

Features of live cell fluorescence imaging活细胞荧光成像的特点Adistribution分布Bcorrelation关联

Features of live cell fluorescence imaging活细胞荧光图像的特征Aquantification定量Bmulti-tracking多重标记跟踪

Step 1:Live Cell Fluorescence Marking活细胞荧光标记标记后标记前

STEP1for Live cell fluorescence imaging活细胞荧光成像第一步：Live-staining with fluorescent dyes or proteins用荧光染料或蛋白对活细胞进行染色Bathstaining全染色Genetransfection基内转染Photoactivation光活化Microinjection显微注射

STEP 2 for Live cell fluorescence imaging阶段2活细胞荧光图像Excitation（llumination）modes照明模式全反射式共聚焦式落射式Zresolution分辨率3um>1.μm<1umS/Nratio比列△O

Step 3:Stimulation & Cellular Responses第三步：刺激细胞，使之发生反应福刺激前刺澈后

STEP 3for Live cell fluorescence imaging活细胞荧光成像第三步Cell stimulation and response细胞刺激和应答Puff pipettingBath application注管吹入全染应用PhotostimulationElectrical stimulation光刺激电子刺激