麻省理工大学：《生物材料——组织交互作用》教学讲义（英文版）Methodology of Organ Synthesis

Methodology of Organ Synthesis A skin B Conjunctiva C Peripheral nerves D Simplest synthetic pathways

Methodology of Organ Synthesis A. Skin B. Conjunctiva C. Peripheral nerves D. Simplest synthetic pathways

The tissue triad in skin and nerves Nerve Skin Axon epidermis∠ basement membrane Basement membrane o. Myelin sheath o Endoneurium dermis 。 Perineurium

The tissue triad in skin and nerves

A. skin synthesis in vivo or regeneration(Ch 5) 1. Experimental parameters 2. Synthesis of epidermis and Bm 3. Synthesis of dermis 4. Partial synthesis of skin 5. Comparative regenerative activity

A. Skin synthesis in vivo or regeneration (Ch. 5) 1. Experimental parameters 2. Synthesis of epidermis and BM 3. Synthesis of dermis 4. Partial synthesis of skin 5. Comparative regenerative activity

1. Experimental parameters (skin A. anatomically well-defined defect -Designate experimental volume -Delete nonregenerative tissue(s) -Anatomical bounds Containment of exudate B. Timescale of observations Initial state: defect generated Final state: defect closed Note: Remodeling continues after closure

1. Experimental parameters (skin) A. Anatomically well-defined defect –Designate experimental volume –Delete nonregenerative tissue(s) –Anatomical bounds –Containment of exudate B. Timescale of observations –Initial state: defect generated –Final state: defect closed Note: Remodeling continues after closure

Image removed due to copyright considerations See Figure 3.1 in Yannas, L.V. Tissue and Organ Regeneration in Adults. New York: Springer-Verlag, 2001

Image removed due to copyright considerations. See Figure 3.1 in Yannas, I. V. Tissue and Organ Regeneration in Adults. New York: Springer-Verlag, 2001. Image removed due to copyright considerations. See Figure 3.1 in Yannas, I. V. Tissue and Organ Regeneration in Adults. New York: Springer-Verlag, 2001

Standardized reactors pider SKIN dermis PERIPHERAL nerve stumps distal proximal NERVE

Standardized reactors • Transected nerve SKIN PERIPHERAL NERVE dermis epidermis nerve stumps

1. Experimental parameters (skin)cont C. Assays of configuration(final state Literature describes several assays, unrelated to nature of product synthesized (e.g. time of closure by epithelialization y take of graft, ability to cross histocompatibility barriers) Required assays are both qualitative( which tissue was synthesized? and quantitative (How much?)

1. Experimental parameters (skin) [Cont.] C. Assays of configuration (final state) -Literature describes several assays, unrelated to nature of product synthesized (e.g., time of closure by epithelialization, % take of graft, ability to cross histocompatibility barriers). -Required assays are both qualitative (which tissue was synthesized?) and quantitative (How much?)

Defect closure rule c+S+R=100 Assays for C, s and R: see ch 4

Defect closure rule C + S + R = 100 Assays for C, S and R: see Ch. 4

spontaneous healing of full-thickness skin excision by contraction and scar formation

spontaneous healing of full-thickness skin excision by contraction and scar formation

Measure c Image removed due to copyright considerations See Figure 4.1 in [ Yannas

Measure C Image removed due to copyright considerations. See Figure 4.1 in [Yannas]. Image removed due to copyright considerations. See Figure 4.1 in [Yannas]