麻省理工大学：《生物材料——组织交互作用》教学讲义（英文版）DENTAL TISSUE REPLACEMENT AND REGENERATION

Massachusetts Institute of Technology VE RII! TAS Harvard medical school Brigham and Womens/Massachusetts General Hosp VA Boston Healthcare System 279J/396J/BE441HST522J DENTAL TISSUE REPLACEMENT AND REGENERATION M. Spector, Ph. D, and I.v.Yannas, Ph.D

Massachusetts In Massachusetts Institute of Technology stitute of Technology Harvard Medical School Harvard Medical School Brigham and Wo Brigham and Women’s/Massachusetts General Hosp. men’s/Massachusetts General Hosp. VA Boston Healthcare System VA Boston Healthcare System 2.79J/3.96J/BE.441/HST522J 2.79J/3.96J/BE.441/HST522J DENTAL TISSUE REPLACEMENT DENTAL TISSUE REPLACEMENT AND REGENERATION AND REGENERATION M. Spector, Ph.D. and I.V. Yannas, Ph.D. M. Spector, Ph.D. and I.V. Yannas, Ph.D

Enamel Crown Dentin Pulp Root Cementum Periodontal membrane ● Nerve and blood suppl OCW

Image source: OCW

Dental Implant DesignsSapphire and materials Alumina Titanium b arbon Images removed due to copyright considerations Alumina Alumina Carbon

Dental Implant Designs and Materials Sapphire Alumina Titanium Car bon Images rem oved due to copyright considerations. Alumina Alumina Car bon

Blade Implant Images removed due to copyright considerations Commercially pure” Titanium

Blade Implant Images removed due to copyright considerations. “Commercially pure” Titanium

Two-Stage Design to shield the artificial root from loading during the initial stage of healing Images removed due to copyright considerations

Two-Stage Design; to shield the artificial root from loading during the initial stage of healing Images rem oved due to copyright considerations

Dental Implant Designs and materials Hydroxyapatite Coated Implants mages removed due to copyright considerations

Dental Implant Designs and Materials Hydroxyapatite￾Coated Implants Images rem oved due to copyright considerations

Calcium Dental hydroxide Caries liner Secondary Osteodentin dentin Secondary dentil Tissue Engineering Enamel Regeneration Dentin Regeneration Pulp Regeneration Restorative Materials Ceramics: porcelain Metals: amalgam, Implants Polymer: composite, gutta percha

Secondary Secondary dentin Dental Caries Osteodentin Osteodentin Calcium Calcium hydroxide hydroxide liner Secondary Secondary dentin Tissue Engineering Enamel Regeneration Dentin Regeneration Pulp Regeneration Restorative Materials Ceramics: porcelain Metals: amalgam, Implants Polymer: composite, gutta percha

ELEMENTS FOR TISSUE REGENERATION/ENGINEERING CELLS Autologous, Adult (pulp and bone marrow stromal stem cell) MATRIX Collagen-GAG CYTOKINES Regulation of phenotype Matrix biosynthesis

ELEMENTS FOR TISSUE ELEMENTS FOR TISSUE REGENERATION/ENGINEERING REGENERATION/ENGINEERING CELLS Autologous, Adult Autologous, Adult (pulp and bone marrow (pulp and bone marrow stromal stem cell) stromal stem cell) MATRIX Collagen Collagen -GAG CYTOKINES CYTOKINES Regulation of phenotype Regulation of phenotype Matrix biosynthesis Matrix biosynthesis

Tissue Engineering of complex tooth structures on Biodegradable polymer scaffolds Cells dissociated from porcine third molar tooth buds Cells seeded onto PLA fiber mesh and implanted in rats for 20 to 30 wks Resulting tooth structures contained dentin (odontoblasts), a well-defined pulp chamber, putative cementoblasts, and a morphologically correct enamel Results suggest the presence of epithelial and mesenchymal dental stem cells in porcine third molar tissues C.S. Young, et al. J Dent Res 81(10): 695-700, 2002

Tissue Engineering of Complex Tooth Structures Tissue Engineering of Complex Tooth Structures on Biodegradable Polymer Scaffolds • Cells dissociated from porcine third molar tooth Cells dissociated from porcine third molar tooth buds. • Cells seeded onto PLA fiber mesh and implanted in Cells seeded onto PLA fiber mesh and implanted in rats for 20 to 30 wks. rats for 20 to 30 wks. • Resulting tooth structures contained dentin Resulting tooth structures contained dentin (odontoblasts odontoblasts), a well ), a well-defined pulp chamber, putative defined pulp chamber, putative cementoblasts cementoblasts, and a morphologically correct enamel. , and a morphologically correct enamel. • Results suggest the presence of epithelial and Results suggest the presence of epithelial and mesenchymal dental stem cells in porcine third molar mesenchymal dental stem cells in porcine third molar tissues. tissues. on Biodegradable Polymer Scaffolds C.S. Young, et al. C.S. Young, et al. J Dent Res 81(10): 695 81(10): 695 -700, 2002 700, 2002

Growth of porcine Enamel, Dentin-, and Cementum-Derived cells in Collagen-GAG Matrices in vitro Unerupted Porcine Premolars and molars Lower mandibles from 6-month old pigs. In aseptic environment, mandibles were split in half, soft tissue removed, and overlying bone from lingual side chiseled away. Exposed teeth were excised and gingiva removed Marty-Roix R, et al, Tiss. Engr. (In press)

Growth of Porcine Enamel Growth of Porcine Enamel-, Dentin , Dentin -, and Cementum Cementum -Derived Cells in Collagen Derived Cells in Collagen -GAG Matrices Matrices In Vitro In Vitro Unerupted Porcine Premolars and Molars Unerupted Porcine Premolars and Molars • Lower mandibles from 6 Lower mandibles from 6 -month old pigs. month old pigs. • In aseptic environment, mandibles were split In aseptic environment, mandibles were split in half, soft tissue removed, and overlying in half, soft tissue removed, and overlying bone from lingual side chiseled away. bone from lingual side chiseled away. • Exposed teeth were excised and gingiva Exposed teeth were excised and gingiva removed. removed. Marty -Roix R, et al., Tiss. Engr. (In press) . (In press)