《植物育种学》课程教学资源（PPT课件讲稿，英文版）CHAPTER 12 APPLICATION OF BIOTECHNOLOGY IN PLANT BREEDING

CHAPIN 小NBR三EDN 生物技在有神中的应周 Dept of Plant Breeding and Seed Engineering

CHAPTER 12 APPLICATION OF BIOTECHNOLOGY IN PLANT BREEDING 生物技术在育种中的应用 BY Niu Yingze （牛应泽） Dept. of Plant Breeding and Seed Engineering

8 1Application of Cell and Tissue Culture in Plant Breeding 细胞组织培养在植物育种的应用 PLANT TISSUE CULTURE is a blanket term for the in vitro cultivation of all plant parts under aseptic conditions. "plant tissue culture," is used as a blanket term for protoplast, cell, tissue and organ curture under aseptic conditions Tissue culture is a technique of growing plant tissues on synthetic medium under controlled and aseptic conditions

§1 Application of Cell and Tissue Culture in Plant Breeding 细胞组织培养在植物育种的应用 PLANT TISSUE CULTURE is a blanket term for the in vitro cultivation of all plant parts under aseptic conditions. Tissue culture is a technique of growing plant tissues on synthetic medium under controlled and aseptic conditions

1. Types of Explants in Plant Tissue Culture 植物组织培养中的外植体类型 · Plantlet Organs(Roots, hypocotyls, cotyledons, leaves, ovary, ovule, anther, pistils, etc.) Embryos(ncl. immature seeds) Tissue(Shoot tip Root tip, Meristem, callus, stem(nodal) segment) Cell(cell suspension) · Protoplast Microspore (Young pollens)

1. Types of Explants in Plant Tissue Culture 植物组织培养中的外植体类型 • Plantlet • Organs (Roots, hypocotyls, cotyledons, leaves, ovary, ovule, anther, pistils, etc.) • Embryos (Incl. immature seeds) • Tissue (Shoot tip, Root tip, Meristem, callus, • stem (nodal) segment) • Cell (Cell suspension) • Protoplast • Microspore (Young pollens)

Ovary culture

Ovary Culture

成热种面 力胚田 Immature Embryo culture

Immature Embryo Culture

Stem Segment Culture

Stem Segment Culture

Hypocotyl Culture Cotyledon Culture

Hypocotyl Culture Cotyledon Culture

4 Microspore Culture

Microspore Culture

2. Application of In vitro Culture in Plant Breeding植物离体培养在育种中的应用 (1) Micropropagation (2)Utilization of Somaclonal Variation ( 3) Protoplast Culture and somatic hybridization (4)In vitro Production of Haploids (5) Embryo Rescue in wild Crosses (6) Transgenic Approach for Plant breeding (7 Germplasm preservation

2. Application of In vitro Culture in Plant Breeding 植物离体培养在育种中的应用 (1) Micropropagation (2) Utilization of Somaclonal Variation (3) Protoplast Culture and Somatic Hybridization (4) In vitro Production of Haploids (5) Embryo Rescue in Wild Crosses (6) Transgenic Approach for Plant breeding (7)Germplasm preservation

3. Utilization of somaclonal variation in Plants植物无性系变异的利用 Somaclonal variation is the variation among the tissues or plants derived from the in vitro somatic cell cultures, i.e. callus and suspension cultures a Somaclonal variation may be genetic or it may also result from culture induced epigenetic(外成的 changes

3. Utilization of Somaclonal Variation in Plants 植物无性系变异的利用 Somaclonal Variation is the variation among the tissues or plants derived from the in vitro somatic cell cultures, i.e. callus and suspension cultures Somaclonal Variation may be genetic or it may also result from culture induced epigenetic(外成的） changes