中国人民解放军陆军军医大学（第三军医大学）：《生物科学综合》课程教学课件_04 干细胞的研究与进展 Stem Cell

Stem Cell Department of cell biology Lian xiao hua TEL:752253

Stem Cell Department of cell biology Lian Xiao Hua TEL:752253

Contents Outline of stem cell Definition of stem cells, Morphology and biochemistry characters, Location and types of stem cell role of stem cell 2. The basic biological features of stem cell (key point)

2 Contents 1. Outline of stem cell Definition of stem cells、 Morphology and biochemistry characters、Location and types of stem cell、Role of stem cell 2.The basic biological features of stem cell (key point)

1. Outline of stem cell stem cell What Where? ( Why? terminally differentiated stem cells 3

3 1. Outline of stem cell stem cells What ? Where? Why?

1. 1 Definition of stem cells Stem cells are cellls found in most multi-cellular organisms, which have the ability to renew themselves and differentiate into a diverse range of specialized cell types 干细胞:具有自我更新能力、并可分化产生某种(或多种) 特化细胞的原始细胞

4 Stem cells are cells found in most multi-cellular organisms, which have the ability to renew themselves and differentiate into a diverse range of specialized cell types. 1. 1 Definition of stem cells 干细胞：具有自我更新能力、并可分化产生某种（或多种） 特化细胞的原始细胞

1.2 Morphology and biochemistry characters of stem cells morphology characters small size larger karyoplasmic ratio fewer organelleS(Er, GC, MT) stem cells

5 morphology characters ： small size larger karyoplasmic ratio fewer organelles(ER,GC,MT) 1.2 Morphology and biochemistry characters of stem cells stem cells

m transmission electron microscopy (TEM image of embryonic stem cells left: liver cell right: embryonic stem cells

6 transmission electron microscopy (TEM) image of embryonic stem cells . left: liver cell right: embryonic stem cells

biochemical marker:胚胎干的Oct-4、SSEA-1、TRA-1-60 TRA-1-81;造血干的端粒酶〔 telomerase);神经干的 nestin、EGF 受体和FGF-2;毛囊干的β1- integrin、CD34等。 mouse embryonic stem cells with fluorescent marker

7 biochemical marker：胚胎干的Oct-4、 SSEA-1、TRA-1-60、 TRA-1-81；造血干的端粒酶（telomerase）；神经干的nestin、EGF 受体和FGF-2； 毛囊干的β1-integrin、CD34等。 mouse embryonic stem cells with fluorescent marker

1.3 Location and types of stem cell location: embryo、 infant、 adult types: embryonic stem cell\ adult stem cell Single Cell Embryo 5-7 Day Embryo Infant Adult Totipotent Embryonic Stem(ES)Cells “Adu|t" Stem cells Pluripotent Multipotent Cord Blood stem cells Placental Stem Cells Multipotent origin of stem cell

types: embryonic stem cell、adult stem cell 1.3 Location and types of stem cell origin of stem cell location: embryo 、infant 、 adult

(1) The embryonic stem cell 胚胎干细胞 P Embryonic stem cells (ES cells) are pluripotent stem cells derived from the inner cell mass of the blastocyst, an early-stage embryo Blastocysts Contain Pluripotential Stem Cells About 4 days in cultur Morula 来自胚泡内细胞团或早期 胚胎; Cell Mass 具有多向分化潜能。 trophoblast 3 incubator embryonic stem cell

9 ◆Embryonic stem cells (ES cells) are pluripotent stem cells derived from the inner cell mass of the blastocyst, an early-stage embryo. embryonic stem cell （1）The embryonic stem cell 胚胎干细胞 来自胚泡内细胞团或早期 胚胎； 具有多向分化潜能

来自胚泡内细胞团或早期胚胎 具有多向分化潜能。 Blastocyst Human Fetus Pluripotent Inner Mass Cells irculatory System Immune System Nervous System embryonic stem cell

10 embryonic stem cell 来自胚泡内细胞团或早期胚胎； 具有多向分化潜能