中国人民解放军陆军军医大学（第三军医大学）：研究生《生命科学综合》核心课程PPT教学课件_04 序列比对与数据库搜索 Sequence Alignment and Database Searching

序列比对与数据库搜索 Sequence alignment and Database Searching 邹凌云副教授 College of basic medical sciences. TMMU E-mail:lingyun.zou@gmail.com Ce15023108658:Tel:771932 http://bioinfo.tmmu.edu.cn

序列比对与数据库搜索 Sequence Alignment and Database Searching 邹凌云 副教授 College of Basic Medical Sciences, TMMU E-mail: lingyun.zou@gmail.com Cell: 15023108658; Tel: 771932 http://bioinfo.tmmu.edu.cn

参考文献 B Altschul SF, et al. Basic local alignment search tool. J Mol Bi.1990Oct5;2l5(3):403-10 B Ben Langmead, Cole Trapnell, Mihai Pop and Steven L Salzberg. Ultrafast and memory-efficient alignment of short dNa sequences to the human genome. genome Biol0gy,2009,10:R25 Genomics and Bioinformatics 2015. TMML

Genomics and Bioinformatics 2015, TMMU 参考文献  Altschul SF, et al. Basic local alignment search tool. J Mol Biol. 1990 Oct 5;215(3):403-10  Ben Langmead, Cole Trapnell, Mihai Pop and Steven L Salzberg. Ultrafast and memory-efficient alignment of short DNA sequences to the human genome. Genome Biology, 2009, 10:R25

Outline 双序列比对介绍 BLAST的基本原理 3 BLAST应用 序列相似度聚类 Genomics and Bioinformatics 2015. TMML

Genomics and Bioinformatics 2015, TMMU Outline 1 双序列比对介绍 2 BLAST的基本原理 3 BLAST应用 4 序列相似度聚类

双序列比对介绍 BLAST的基本原理 3 BLAST应用 序列相似度聚类

1 双序列比对介绍 2 BLAST的基本原理 3 BLAST应用 4 序列相似度聚类

Assumptions Life is monophyletic Biological entities share common ancestry Genomics and Bioinformatics 2015. TMML

Genomics and Bioinformatics 2015, TMMU Assumptions Life is monophyletic Biological entities share common ancestry

ancestor Any two organisms or two sequences share a common ancestor in their past descendant 1 descendant 2

6 Any two organisms or two sequences share a common ancestor in their past ancestor descendant 1 descendant 2

nestor (5 MYA, Million Years Ago)

7 ancestor (5 MYA, Million Years Ago)

ancestor (120MYA)

8 ancestor (120 MYA)

ancestor (1500MYA)

ancestor (1500 MYA)

Whale Generalized y Homology Aterm was coined by Richard Owen in 1843 Radius TIna 23 Definition: Similarity Carpels (wrist) OQO Metacarpals(palm) resulting from common Phalanges(digits)00000 H ancest Bat bones)

Homology: Aterm was coined by Richard Owen in 1843. Definition: Similarity resulting from common ancestry