中国人民解放军陆军军医大学（第三军医大学）：研究生《生命科学综合》核心课程PPT教学课件_11 细胞重要生命活动及其研究（2/2，王韵）

三涅民驾种谓 C/2 COLLEGE OF MEDICINE THIRD MIILITARY MEDICAL UNIVERSITY 细胞重要生命活动及其研究 Department of cell biology Yun Wang Email:yunwang@tmmueducn Tel:68752252

THIRD MIILITARY MEDICAL UNIVERSITY COLLEGE OF MEDICINE THIRD MIILITARY MEDICAL UNIVERSITY COLLEGE OF MEDICINE Department of cell biology Yun Wang Email:yunwang@tmmu.edu.cn Tel: 68752252 细胞重要生命活动及其研究

细胞社会性 Cell sociology ◆细胞与细胞、细胞与细胞外基质乃至整个 机体的相互作用、相互依存、相互制约即细 胞的社会性。 ◆细跑的生存、生长、分裂、分化等行为必 须在细胞社会中进行。 正常细胞繁殖接受细胞社会的约束,一切有条不紊

◆ 细胞与细胞、细胞与细胞外基质乃至整个 机体的相互作用、相互依存、相互制约即细 胞的社会性。 1、细胞社会性 Cell sociology ◆ 细胞的生存、生长、分裂、分化等行为必 须在细胞社会中进行

Cell sociology Cell-cell interreaction 细胞间直接接触、细胞通过分泌介质相互影响 Cell-ecM interreaction mechanical stresses are transmitted from epithelial cell to cell by cytoskeletal tissue filaments anchored to cell-matrix and cell-cell basal adhesion sites lamina extracellular matrix directly bears mechanical connective stresses of tension and tissue compression collagen fibers Interreaction of cell-cell or cell-ECM

◆ Cell-cell interreaction 细胞间直接接触、细胞通过分泌介质相互影响 ◆ Cell-ECM interreaction Interreaction of cell-cell or cell-ECM Cell sociology

1.1 Cell adhesion and cell Junction The cellular property that mediates the binding is called cell adhesion Many cells in tissues are linked to one another and to the extracellular matrix at specialized contact sites called cell unctions

1.1 Cell Adhesion and Cell Junction ◆ Many cells in tissues are linked to one another and to the extracellular matrix at specialized contact sites called cell junctions. ◆ The cellular property that mediates the binding is called cell adhesion

三涅民驾种谓 C/2 COLLEGE OF MEDICINE THIRD MIILITARY MEDICAL UNIVERSITY Cel△ dhesion epidermis mesoderm Neural tube Cell recognition and cell adhesion

THIRD MIILITARY MEDICAL UNIVERSITY COLLEGE OF MEDICINE THIRD MIILITARY MEDICAL UNIVERSITY COLLEGE OF MEDICINE Cell Adhesion Cell recognition and cell adhesion mesoderm epidermis Neural tube

Overview of cell adhesion Cell adhesion molecules(CAM) Table 19-5 Cell Adhesion Molecule Families SOME FAMILY Ca2+ OR Mg 2+ HOMOPHILIC ORCYTOSKELETON CELL JUNCTION MEMBERS DEPENDENCE HETEROPHILIC ASSOCIATIONS ASSOCIATIONS Cell-Cell Adhesion Classical cadherins E, N, P VE homophilic actin filaments(via adherens junctions, catenins Desmosomal desmoglein, yes homophilic intermediate filaments desmosomes cadherins desmocollin (via desmoplakin, plakoglobin, and plakophilin) Ig family members N-CAM, ICAM no both unknown neuronal and immunological synapses Selectins(blood cells L,E, and heterophilic actin filaments (no prominent junctional and endothelial cells P-selectins only) Integrins on blood cells aLB2(LFA1)yes heterophilic actin filaments immunological synapses Cell-Matrix Adhesion Integrins many types ye heterophilic actin filaments(via focal adhesions talin, paxillin, filamin a-actinin, and vinculin) 4 heterophilic intermediate filaments hemidesmosomes (via plectin and dystonin) Transmembrane syndecans heterophilic actin filaments (no prominent junctional proteoglycans structure) Table 19-5 Molecular Biology of the Cell 5/e(o Garland Science 2008)

Cell adhesion molecules(CAM) Overview of cell adhesion

Selectins, integrins and Ig- super family mediate the cell-cell adhesions basal lamina WEAK ADHESION STRONG ADHESION AND ROLLING AND EMIGRATION selectin-dependent)(integrin-dependent)blood vessel white blood cell endothelial cell tis ssue

Selectins,integrins and Ig-superfamily mediate the cell-cell adhesions

Cell Junction tight junction actIn fiLaments dhesion belt (cadherins) cadherins 8 desmosome (cadherins) Ig-like CAMS integrins inter- mediate gap junction selectins filaments (connexins) hemidesmosome focal contact integral (integrins (integrins membrane proteoglycan integrins basal lamina ↓ JUNCTIONAL ADHESION MECHANISMS NONJUNCTIONAL ADHESION MECHANISMS

Cell Junction

三涅民驾种谓 C/2 COLLEGE OF MEDICINE THIRD MIILITARY MEDICAL UNIVERSITY 1.2 Cell and extracellular matrix Scanning electron micrograph of fibroblasts in connective tissue .o um of their volume is extracellular space, which is large/ r issues are not made up solely of cells. A substantial pa filled by an intricate network of macromolecules constituting the extracellular matrix

THIRD MIILITARY MEDICAL UNIVERSITY COLLEGE OF MEDICINE THIRD MIILITARY MEDICAL UNIVERSITY COLLEGE OF MEDICINE 1.2 Cell and Extracellular Matrix Scanning electron micrograph of fibroblasts in connective tissue. Tissues are not made up solely of cells. A substantial part of their volume is extracellular space, which is largely filled by an intricate network of macromolecules constituting the extracellular matrix

ECM: an intricate network of macromolecules Polysaccharide chain: Glycosaminoglycan(GAG)氨基聚糖 proteoglycan(PG)蛋白聚糖 Fibrous proteins: Collagen(CL), elastin Fibronectin(Fn), laminin(Ln 胶原 纤粘连蛋白 纤粘连 蛋白聚糖 小D MD5 《《福 WD 整联蛋白

ECM: an intricate network of macromolecules . Polysaccharide chain: Glycosaminoglycan (GAG) 氨基聚糖 proteoglycan (PG) 蛋白聚糖 Fibrous proteins: Collagen (CL), elastin Fibronectin (FN), laminin (LN)