《病原生物学》课程教学课件（2010）细菌感染的检查方法与防治原则

TrialversieConverter.http://www.okdosoft.com第8章细菌感染的检查方法与防治原则Examinationand strategyofprevention and therapy for bacterialinfection陈雪玲石河子大学医学院

第一节细菌感染的检查TrialversionofOkdoDocPptJpegWmftoPdf Converter.http://www.okdosoft.com病原菌检测（一）标本采集与运送原则早期、无菌、尽快送检二细菌分离培养与鉴定1.分离培养2.形态学检查3.生化试验4.血清学试验5.药物敏感试验

形态学检查examinationin morphologyTrial versionofOkdoDocPptJpeg Wmfto Pdf Converter.http://www.okdosoft.com直接涂片镜检：染色法：革兰染色法，抗酸染色法不染色法：悬滴法、压片法负染色法：墨汁压片

Ocularlens(eyepiece)RemagnifiestheimageformedbytheobjectivelensBodytubeTransmitstheimagefromthe ob-tnuareTrial versionofOkdoDocPptJpegWmftoPdfConverter.http://www.okdosoft.comObjectivelensesPrimaryJensesthatmagnifythespecimenStage Holds themicroscopeslideinpositionCondenserFocuseslightthroughspecimenDiaphragmControlstheamountof light.enteringthecondenserCaarsefocusingknoblluminatorLightsourceBaseFinefocusingknob(a) Principal parts and functionsFigure3.1ThecompoundlightmicroscopeHowisthetotalmagnification ofacompound liglit microscopecaleulated

分离培养isolationandcultivationTrial versionofOkdoDocPptJpeg Wmfto Pdf Converter.http://www.okdosoft.com分离培养是微生物学诊断的金标准，是最可靠的传统诊断技术。图为普通孵箱）

Trial versionofOkdoDocPptJpegWmftoPdfConverter.http:l/www.okdosoft.com厌氧袋厌氧箱

生化反应Trial versionofOkdoDocPptJpegWmftoPdf Converter.http://www.okdosoft.com是鉴定细菌的重要方法之一。针对一个菌落或纯培养物进行鉴定

TrialversionofOkdoDocPptJpegWmftoPdf Converter.http://www.okdosoft.com（三）病原菌抗原的检测ElA,IF,Westernblotting（四）检测病原菌核酸核酸杂交PCR基因芯片（genechips）