扬州大学：《生物化学 Biochemistry》课程教学课件（讲稿）Experiments for Biochemsitry 1_Miscellaneous mistakes

Miscellaneous mistakes

Miscellaneous mistakes

['pipet]dropper, pipette, or dropping pipetteprecautionstoensure accuracy and prevent contamination.Alwaysholdthedropperverticallyandsteadily,withthetip suspended justabovethe containertoavoid touchingthesidesorbottom,whichcouldintroduceimpurities.-minimizetheriskofexternalcontaminationAftereachuse,properlycleanthe dropper, ensuringthoroughrinsingtoavoidcross-contaminationbetweendifferentliquids.Fordroppersintendedforspecificuses,suchasthoseprovidedwithdropperbottles,avoidusingthemforotherpurposestomaintaintheirintegrity and dedicatedfunction

dropper, pipette, or dropping pipette  precautions to ensure accuracy and prevent contamination.  Always hold the dropper vertically and steadily,  with the tip suspended just above the container to avoid touching the sides or bottom, which could introduce impurities. — minimize the risk of external contamination.  After each use, properly clean the dropper, ensuring thorough rinsing to avoid cross-contamination between different liquids.  For droppers intended for specific uses, such as those provided with dropper bottles, avoid using them for other purposes to maintain their integrity and dedicated function. ['pɪpɛt]

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pipette ['prpet]Toensureaccurateand contamination-freeliquidtransferAlways ensurethatthe pipette is clean and drybeforeuse, as any residualmoistureorcontaminants could affecttheaccuracyofyourmeasurementsWhen aspirating liquid, hold the pipette vertically and immerse only the tipinto the liquid,avoiding contact with the sides of the container to preventcontaminationGently aspirate the liquid by applying suction, and be careful not to overfillthepipettetoavoid spillage.Dispense the liquid slowly and carefully into the receiving container, ensuringthat the tip of the pipette does not touch the sides or bottom of the container.Pipettes aredesignedfor specific volumesand shouldnotbeusedinterchangeably.rubber suction bulAlwaysusethepipettethatcorrespondstothevolumeyouneedtomeasure>Afteruse,cleanthepipettethoroughly,ensuringcompleterinsingtopreventcross-contamination

pipette ['pɪpɛt] To ensure accurate and contamination-free liquid transfer.  Always ensure that the pipette is clean and dry before use, as any residual moisture or contaminants could affect the accuracy of your measurements.  When aspirating liquid, hold the pipette vertically and immerse only the tip into the liquid, avoiding contact with the sides of the container to prevent contamination.  Gently aspirate the liquid by applying suction, and be careful not to overfill the pipette to avoid spillage.  Dispense the liquid slowly and carefully into the receiving container, ensuring that the tip of the pipette does not touch the sides or bottom of the container.  Pipettes are designed for specific volumes and should not be used interchangeably.  Always use the pipette that corresponds to the volume you need to measure.  After use, clean the pipette thoroughly, ensuring complete rinsing to prevent cross-contamination. rubber suction bulb

永远不要始终坚持废液缸

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确超液位刻度正雄

图+1-9移液管冲洗摄作(a)(1)(s)

QualitativeexperimentQuantitativeexperimentPurpose:identify,classify,andPurpose:testhypotheses,establishdescribetheproperties,causalrelationships.ormakecharacteristics,orcomponentsofapredictionsbasedonnumericaldatasubstanceorsystem without(tomeasure and analyze)measuringtheir quantitiesMethods:collectnumericaldataMethods:observations,colorusing scales,indices,or otherchanges,precipitationreactions,andquantifiablemetrics.otherindicatorsthatprovideOutcome:arenumerical and ofteninformationaboutthepresenceorpresented inthe form of statisticalabsenceofcertainsubstances.summaries,graphs,ortables.Outcome:Resultsaretypicallyexpressed in descriptiveterms,suchaspositive/negativereactions,colorchanges, or other qualitativeindicators

Qualitative experiment  Purpose: identify, classify, and describe the properties, characteristics, or components of a substance or system without measuring their quantities.  Methods: observations, color changes, precipitation reactions, and other indicators that provide information about the presence or absence of certain substances.  Outcome: Results are typically expressed in descriptive terms, such as positive/negative reactions, color changes, or other qualitative indicators. Quantitative experiment  Purpose: test hypotheses, establish causal relationships, or make predictions based on numerical data. (to measure and analyze)  Methods: collect numerical data using scales, indices, or other quantifiable metrics.  Outcome: are numerical and often presented in the form of statistical summaries, graphs, or tables