《动物微生物与免疫学》课程PPT教学课件（免疫学基础）第十三章 免疫血清学技术

血清学技术第十三章

第十三章 血 清 学 技 术

ANTIGENANTIBODYNH.OHCH-CH-HydrogenbondCH2CH2CH2NH+onicbondCHCHBCH2OHCH3HydrophobicCH3CH3interactionsCHCTL2CH3vanderWwaalsCHCH3CHCHinteractionsCHCH3-0lonic bondC2CH2HN抗原抗体的的结合力

抗原抗体的的结合力

(b)Antibody-excessEquivalenceAntigen-excesszonezonezoneexcessAhSupernatantsexcessAgAntibodyprecipitatedAntigenadded抗原抗体结合的最适比与带现象

抗原抗体结合的最适比与带现象

RADATNOIEFSONAntieendiffusionAntibodlyincorporatedAntigenin agarPrecipitateformsringTOTBIETTNAOeAgarnmatrixPrecipitate沉淀反应示意图

沉淀反应示意图

(b)(a)Antigens with epitope AAnti-B-BAnti-A-ABAntigen1AntigenAA-withwith--cpitope BepitopeABAnti-A-AAnti-B-BDiffusingAnti-A-AprecipitateantigenprecipitateprecipitateDiffusing antibodyAnti-BAnti-AAnti-AantiserumIDENTITYNONIDENTITY琼脂扩散的形式

琼脂扩散的形式

(c)Anti-A-Aprecipitate spurBBAAntigenAntigenwithwithepitopeBepitopesBBAandBAnti-B-BprecipitateAnti-A-AB/SAnti-B-AB-precipitateAnti-BAnti-APARTIALIDENTITY琼脂扩散的形式

琼脂扩散的形式

a)lgGAntigensIgMAntibodkigA免疫电泳

免疫电泳

(a)IndirectELISAwashwashwashAdd specificAdd enzymeAdd substrate (S)Antigencoated wellantibody to beconjugatedandmeasurecolormeasuredsecondaryantibody间接ELISA

间接ELISA

CellswithmembranePrimaryantibodyantigens (mAg)tomAgSecondaryPrimaryProteinAanti-isotypeantibodyantibodylndirectmethodwithfluorochrome(a)Directmethodwithfluorochrome-(b)Indirectmethodwithfluorochrome-labeledproteinAlabeledantibodytomAglabeledanti-isotypeantibody荧光抗体标记技术(d)

荧光抗体标记技术

(b) Sandwich ELISAwaswasAntibody-Add enzyme-Add substratecoated wellconjugatedand measurecolorsecondary antibody夹心ELISA

夹心ELISA