安徽医科大学：《医学细胞生物学》课程教学资源（课件讲稿，打印版）Extracellular matrix

Extracellular Matrix (ECM) Collagen Fibronectin Laminin Proteoglycan D 6d86的 This document is produced by trial version of Print2Flash.Visit www.print2flash.com for more information

ECM Concept:An orderly network outside cells,consisting of secreted protein and polysaccharide by cells. Function:Adhesion,support,regulate the microenvironment of cells,signaling transition,cell proliferation,differentiation, survival,and death. Types:Glycosaminoglycan proteoglycans,collagen elastin, fibronectin laminin,and the plant cell wall. This document is produced by trial version of Print2Flash.Visit www.print2flash.com for more information

Contents Extracellular cell matrix 1 Components of ECM Basal lamina 3 Interaction between ECM and cells This document is produced by trial version of Print2Flash.Visit www.print2flash.com for more information

Components of ECM ECM Structure Gel-like matrix Fiber network Molecules GAG PG Collagen elastin fibronectin laminin This document is produced by trial version of Print2Flash.Visit www.print2flash.com for more information

1.Glycosaminoglycan (GAG)&Proteoglycans (PG) Amino polysaccharide is a straight polysaccharide chain constituted by repeated disaccharide units. GAG disaccharide units sulfate groups Distribution per unit Hyaluronan Glucuronic acid-N-acetyl 0 Connective tissue,skin, glucosamine cartilage,synovial fluid vitreous Chondroitin Glucuronic acid-N-acetyl 0.2-2.3 Cartilage,cornea,bone,skin, sulfate galactosamine arterial Dermatan Glucuronic acid or Aidoo 1.0-2.0 Skin,blood vessels,heart, sulfate uronic acid-N-acetyl valve 出到 galactosamine heparan Glucuronic acid or Aidoo 0.2-3.0 lung artery,and cell surface sulfate uronic acid-N-acetyl glucosamine Heparin Glucuronic acid or Aidoo 2.0-3.0 Lung,liver,skin,mast cells uronic acid-N-acetyl glucosamine This document is produced by trial version of Print2Flash.Visit www.printflash.com for more information

Proteoglycans (PG) 1μm aggrecan aggregate High molecular weight complexes of GAG core protein covalently bind with (aggrecan) core proteins; Structure link protein hyaluronan molecule keratan sulfate chondroitin sulfate This document is produced by trial version of Print2Flash.Visit www.print2flash.com for more information

Proteoglycans (PG) Sythesized and assembled in ER 1)Polysaccharides are assembled to core protein in ER lumen by glycosyltransferases,which in turn add monosaccharide to the specific tetrasaccharide structure on serine; 2)Polymorphology of amino acid sequence and sugar chain; 3)Some PG are integrated members of plasma membrane (Syndecan,蛋白多糖). This document is produced by trial version of Print2Flash.Visit www.print2flash.com for more information

Function of GAG and PG 1.Make the tissues have flexibility and resistance to stress; 2.Selective permeability of the material transport (glomerulus,.肾小球)； 3.Proteoglycans in the cornea()are translucent; 4.Anticoagulant effect; 5.Signal transition; 6.Invovled in tissue aging. This document is produced by trial version of Print2Flash.Visit www.print2flash.com for more information

GAG PG and disease 1.Abnormal degradation-abnormal accumulation- mucopolysaccharidoses(黏多糖贮积病)； 2.Change of content ratio-lipid deposition-atherosclerosis (动脉粥样硬化)； 3.Secretion increase and level of sulfate reduced-promote tumor cell proliferation and migration -tumor proliferation, invasion,and metastasis. This document is produced by trial version of Print2Flash.Visit www.print2flash.com for more information

2.Collagen and elastin Collagen is the skeleton of ECM The most abundant protein in human. ■ Structure:Triple helix of 3 a-polypeptide strands,the sequence is repeate of Gly-X-Y; There're many types of a strands with different kinds of combination style. Pri-peptide-remove signal peptide-add prepeptide on each ends.—Hydroxylation-—cross link at C terminal(procollagen)-secreted outside-remove prepeptide-self-assembly-mature collagen. This document is produced by trial version of Print2Flash.Visit www.print2flash.com for more information