复旦大学：《医学免疫学 Medical Immunology》课程教学资源（PPT课件讲稿，英文版）Antibodies Antibody（Ab）

e DEPARTMENT OF IMMUNOBIOLOGY Antibody(Ab) Xiaowu hong xiaowuhong@fudna.edu.cn 021-54237093 Department of Immunology Shanghai medical college of Fudan University

DEPARTMENT OF IMMUNOBIOLOGY Antibody (Ab) Xiaowu Hong xiaowuhong@fudna.edu.cn 021-54237093 Department of Immunology Shanghai Medical College of Fudan University

Nobel prize winners Emil von Behring, Paul ehrlich, 1908 Georeges Kohler and cesar Milstein 1901. antitoxins production of antibody 1984, monoclonal antibody Gerald edelman and Rodney porter, 1972. structure of antibody Susumu Tonegama, 1987, structure of ig gene

Emil von Behring, 1901, antitoxins Georeges Kohler and Cesar Milstein, 1984, monoclonal antibody Susumu Tonegama,1987, structure of Ig gene Gerald Edelman and Rodney Porter, 1972, structure of antibody Paul Ehrlich , 1908, production of antibody Nobel Prize winners

Emil von Behring(1845-1917) 八 Tetanus Immunized hors Emil von Behring 1901, antitoxins 恩 Immune horse serum antitoxin) Patient at Patient sk of protecte tetanus

Emil von Behring (1845-1917) Emil von Behring, 1901, antitoxins

Contents I Definition of antibody II Structure of antibody I Function of antibody IV Biological characteristics of different antibodies

I Definition of antibody II Structure of antibody III Function of antibody IV Biological characteristics of different antibodies Contents

Antibody(Ab) a globulin which is produced by plasma cell as a result of the introduction of an antigen and which has the ability to combine with the antigen that stimulated its production x→◎

Antibody (Ab) A globulin which is produced by plasma cell as a result of the introduction of an antigen and which has the ability to combine with the antigen that stimulated its production

Immunoglobulins(Ig Globulins composed of H and L chains, or globulins function as antibody Immunoglobulins Antibody-containing serum is place in an electrical field Anti bodies migrated with the globular proteins

Immunoglobulins (Ig) Globulins composed of H and L chains, or globulins function as antibody. Immunoglobulins Antibody-containing serum is place in an electrical field Antibodies migrated with the globular proteins

The relationship between Ab&z Ig Al antibodies are immunoglobulins, but it is not certain that all immunoglobulins have antibody function

All antibodies are immunoglobulins, but it is not certain that all immunoglobulins have antibody function. The relationship between Ab & Ig

Distribution of antibody Antibody molecules are found in serum (account for approximately 20% of the total plasma protein ) in extravascular fluids. in exocrine secretions. and on the surface of some lymphocytes

Antibody molecules are found in serum (account for approximately 20% of the total plasma protein ), in extravascular fluids, in exocrine secretions, and on the surface of some lymphocytes. Distribution of antibody

Section 1 Structure of Ig Fab L Fab hinge CH1 CH2 U CH3 Fc

Section 1 Structureof Ig

1 Basic four chain structure A four poly peptide chains (b) two identical light chains two identical heavy chains, held by disulfide bonds binding Hinge * Y-shape structure symmetrIc. Biological ctIvity 12 一NH2 terminal, cHe CHO CooH term inal *** variable constant reglons **** domains

* A four polypeptide chains: two identical light chains two identical heavy chains , held by disulfide bonds. ** Y-shape structure, symmetric. *** –NH2 terminal, - COOH terminal. **** variable & constant regions. ***** domains 1 Basic four chain structure