复旦大学：《细胞生物学 Cell Biology》课程教学资源（PPT课件）细胞核和染色体 Nucleus and Chromosomes

Chap 8 Nucleus and Chromosomes Nucleus of a Eukaryotic Cell Nuclear Envelope Nuclear Pore Complex Chromatin Nucleolus and ribosome Biogenesis Nuclear matrix

Chap 8 Nucleus and Chromosomes • Nucleus of a Eukaryotic Cell • Nuclear Envelope • Nuclear Pore Complex • Chromatin • Nucleolus and Ribosome Biogenesis • Nuclear Matrix

8.1 The Nucleus of a Eukaryotic Cell Surrounded by two concentric membranes The outer nuclear membrane

8.1 The Nucleus of a Eukaryotic Cell ❖The inner nuclear membrane ❖ The outer nuclear membrane Surrounded by two concentric membranes

DNA and associated proteins(chromatin endoplasmic reticulum nucleolus 像 centrosome microtubule intermediate filaments nuclear lamina nuclear pore 1 um outer nuclear membrane nuclear envelope inner nuclear membrane

8.1.1 Organization of the Nucleus chromosomes nuclear matrix nucleoli nucleoplasm

8.1.1 Organization of the Nucleus chromosomes nuclear matrix nucleoli nucleoplasm

Heterochro an interphase Hela cell nucleus 1 Nuclear envelope A.Nuclear pore Nucleolus showing some of the major /Nuclear matrix components of the nucleus neoplasm Chromatin

an interphase HeLa cell nucleus showing some of the major components of the nucleus

8.1.2 Internal Architecture contrast to the cytoplasm, the nucleus. not individually enclosed by membranes not visible using conventional light or electron microscopy techniques

8.1.2 Internal Architecture contrast to the cytoplasm, the nucleus: ❖not individually enclosed by membranes ❖not visible using conventional light or electron microscopy techniques

revised picture of nuclear structure Nuclear matrix: a proteinaceous scaffold-like network Nucleolus: the center for synthesis and processing of rRNA molecules

revised picture of nuclear structure • Nuclear matrix: a proteinaceous scaffold-like network • Nucleolus: the center for synthesis and processing of rRNA molecules

8.2 The Nuclear Envelope The separation of a cells genetic material from the surrounding cytoplasm may be single most important feature that distinguishes eukaryotes from prokaryotes

8.2 The Nuclear Envelope • The separation of a cell’s genetic material from the surrounding cytoplasm may be single most important feature that distinguishes eukaryotes from prokaryotes

Structure of the nuclear envelope perinuclear space

Structure of the nuclear envelope • outer nuclear membrane • inner nuclear membrane • nuclear lamina • perinuclear space • nuclear pore

Cytoplasm Integral protein Outer membrane Ribosome Inner membrane Nuclear pore complex ER Intermembrane Lamina space Heterochromatin (a) NPC NM. 4 (b) 0.2um