复旦大学：《人体的社交网络——共生微生物》课程教学资源（文献讨论）Changes in the Eye Microbiota associated with Contact Lens Wearing

Changes in the Eye Microbiota associated with Contact Lens Wearing Hakdong Shin, Kenneth Price, Luong Albert Jack Dodick,Lisa Park, Maria Gloria Dominguez-Bello 陈藤蔡婷陈坷璇刘穹

background result scussIon

Genus Pseudomonas 假单胞菌属 Haemophilus 嗜血杆菌属 Streptococcus 链球菌属 Methylobacterium 甲基杆菌属 Lactobaci∥ls 乳酸菌属 Acinetobacter 不动杆菌属 Staphylococcus 葡萄球菌属 Corynebacterium 棒状杄菌属

O cular Defensive role microbiota mostly coagulasenegative Colonization of pathogens Over 50% growth staphylococci, Propionibacterium, and in the skin-like bacteria Culture-dependent additional Pseudomonas, Bradyrhizobium Aquabacterium, Sphingomonas, Streptococcus, Streptophyta, quencing workCcby-sa3.0,https:// fromen.wikipediatoCommonsbyKauczukusingCommonshelper,CcbY-sa3.,https:/commons.wikimediaorg/w/indexphp?curid=4916101 p//pic35nipic.com/20131109/1246704817115327410 tp: //easyread ph 126. net/qropBurbEtThz05oMQQQrw==/7917002689475514117-jpg

By The original uploader was Lady Weaxzezz at English Wikipedia - Transferred from en.wikipedia to Commons by Kauczuk using CommonsHelper., CC BY-SA 3.0, https://commons.wikimedia.org/w/index.php?curid=4916101 By Aney - Own work, CC BY-SA 3.0, https://commons.wikimedia.org/w/index.php?curid=290822 http://pic35.nipic.com/20131109/12467048_171153274104_2.jpg http://easyread.ph.126.net/qropBurbEt7hz05oMQQQrw==/7917002689475514117.jpg Culture-dependent Sequencing growth of skin-like bacteria mostly coagulasenegative staphylococci, Propionibacterium, and Corynebacterium Pseudomonas, Bradyrhizobium, Acinetobacter, Brevundimonas, Aquabacterium, Sphingomonas, Streptococcus, Streptophyta, Methylobacterium, Enhydrobacter, Bacillus, and Ralstoniaspp additional Over 50% Defensive role against Colonization of pathogens in the eye

A risk factor for inflammatory eye conditions Microbial involvement and interacti 100 million host conjunctival epithelium worldwide Bacterial contamination of contact lenses 16s rrNA *V4 region contact gene sequencing lenses surveys Microbiota y 58 subjecs ,o non-lens wearers 28 lens wearers ocular surface 7, 010,096 sequences Structure function with an average of 21, 569 reads per sample 11, 750 OTUs yielded By-70InxownworkCcBy3.0,https://commons.wikimediaorg/w/indexphp?curid=7929385

~ 100 million worldwide A risk factor for inflammatory eye conditions Microbial involvement and interaction with host conjunctival epithelium Bacterial contamination of contact lenses By - טל איתןOwn work, CC BY 3.0, https://commons.wikimedia.org/w/index.php?curid=7929385 58 subjects 7,010,096 sequences 11,750 OTUs yielded 30 non-lens wearers 28 lens wearers with an average of 21,569 reads per sample without singletons ocular surface *V4 region

PD( phylogenetic diversity )whole tree otu es No orU ut on 20 Number of species Faith D P Conservation evaluation and phyloge rsity]. Biological Conservation, 1992, 61(1): 1

Faith D P. Conservation evaluation and phylogenetic diversity[J]. Biological Conservation, 1992, 61(1):1-10. http://www.berrygenomics.com/wp-content/uploads/2014/12/otu_tree.png

Effect of Anesthetic eye drops PD Whole Tree Observed species 53g5m25四9m Sequences Per Sample uences Per Sampl a diversity Sampling location Laboratory(n=116, conjunctiva Ophthalmology practice(n=75, conjunctiva Fig S1A

Fig.S1A α diversity

Effect of Anesthetic eye drops LDA Phylum-level Genus-level P:g_Pseu Fusobacteria* Cyanobacteria" f Enterobacteriaceae: g Bacteroidetes* P: g_ Neisseria Actinobacteria P: f Oxalobacteraceae Other* P: g_Sphingobium F:g_Anaerococcus :g Streptococcus Fig_Lactobacillus* F:g_Staphylo C; o_Streptophyta; f_:g A; g Rothia Laboratory Ophthalmologic Ophthalmologic g_coryneb Unassigned practice Laboratory practice Conjunctiva Conjunctiva Conjunctiva Conjunctiva n=116 n=75 n=116 Fig S1B

Fig.S1B LDA

Effect of Anesthetic eye drops Unweighted Weighted Pc2(4%) O Ophthalmologic practice Conjunctiva (N=75) O Ophthalmologic practice Conjunctiva(N=75) O Laboratory Conjunctiva(N=116) gl Ophthalmolog O Laboratory Conjunctiva( N=116) Laboratory a Aphtha malacic +-- aboratory 02040.60.8 p=0.001 Pc1(13% Pc1(54%) Fig S1C

Fig.S1C

Figure. PD Whole Tree Observed species Non-lens wearers Conjunctiva Lens wearers Non-lens wearers (n=63) Skin under the eve Lens wearers (n=51) Lens wea Contact lens 300 400 Phylogenetic diversity Number of observed species T-test

T-test