复旦大学：《人体的社交网络——共生微生物》课程教学资源（文献讨论）Helicobacter pyloriinfection can affect energy modulating hormones and body weight in germ free mice

Helicobacter pylori infection can affect energy modulating hormones and body weight in germ free mice 周蔚然14301050248 刘萱琪14301050131 耿子寒14301050232 李涵玉14301050229

Helicobacter pylori infection can affect energy modulating hormones and body weight in germ free mice 周蔚然 14301050248 刘萱琪 14301050131 耿子寒 14301050232 李涵玉 14301050229

B ackgroun 胃肠道菌群( gut microbiota),在胃肠道内发 A挥诸多功能,如分解营养物质、影响能量代谢、 抵抗外来病原等。 幽门螺杆菌( Helicobacter b pylori)是胃部微生物的重要 组成部分

幽门螺杆菌 （Helicobacter pylori）是胃部微生物的重要 组成部分。 A B 胃肠道菌群 （ gut microbiota），在胃肠道内发 挥诸多功能，如分解营养物质、影响能量代谢、 抵抗外来病原等。 Background

Gut microbiota impact on metabolism one study needed 30%o more calories to keep a similar body mass GF another study ate more but had less body fat

Gut microbiota impact on metabolism GF GF needed 30% more calories to keep a similar body mass ate more but had less body fat one study another study

Helicobacter pylori impact on metabolism an inverse correlation between prevalence of the H pylori and rate of obesity H. pylori might regulate ghrelin and leptin levels

1 an inverse correlation between prevalence of the H. pylori and rate of obesity H. pylori might regulate ghrelin and leptin levels. 2 Helicobacter pylori impact on metabolism

B ackgroun Previous study gut microbiota might modulate brain development in early stages of life H. pylori can cause inflammation Need to be researched which activates the immune system the interplay between H pylori and normal gut 02 microbiota whether H. pylori affect growth in early life

Previous study： • gut microbiota might modulate brain development in early stages of life • H. pylori can cause inflammation which activates the immune system 01 02 Need to be researched： • the interplay between H. pylori and normal gut microbiota • whether H. pylori affect growth in early life Background

Aim assess whether a H. pylori infection could affect growth in early life investigate the interplay between H. pylori and normal gut microbiota during early stages of life understand the influences of H pylori and normal gut microbiota on the development of metabolic and immune systems by assessing the metabolic hormones and cytokines

Aim • assess whether a H. pylori infection could affect growth in early life • investigate the interplay between H. pylori and normal gut microbiota during early stages of life • understand the influences of H. pylori and normal gut microbiota on the development of metabolic and immune systems by assessing the metabolic hormones and cytokines

Methods-①分组&取样 SPF SPFH GF GFH py gut microbiota male mice, 4 group, N12/per group, age 2-4 weeks sacrificed in2、8、16 weeks post- colonization fasting plasma(空腹血浆) was used for hormone and cytokine analysis

Methods——①分组＆取样 SPF SPFH GF GFH H. pylori - + - + gut microbiota + + - - • male mice，4 group，N=12/per group，age 2-4 weeks • sacrificed in 2 、8、16weeks post-colonization • fasting plasma （空腹血浆）was used for hormone and cytokine analysis

Methods②检测指标 hormone and cytokine eight gut hormones ghrelin, leptin, amylin, insulin Metabolic system GLP-1,GIP PYY and pp (important regulators of food intake, energy expenditure and body mass) Immune system eotaxin-1

Methods——②检测指标 hormone and cytokine Metabolic system eight gut hormones：ghrelin, leptin, amylin, insulin, GLP-1,GIP, PYY and PP（important regulators of food intake, energy expenditure and body mass） Immune system eotaxin-1

RESULT--GE小鼠体重低于SPF小鼠 Body Weight normal gut microbiota plays an Important role in determining energy harvest from the diet and P. SPF energy storage in its host FH Duration(weeks)

• normal gut microbiota plays an important role in determining energy harvest from the diet and energy storage in its host RESULT——GF小鼠体重低于SPF小鼠

RESULT--GFH小鼠体重低于GF小鼠 Body Weight in the absence of normal gut microbiota, H pylori caused weight loss -Q-0SPF SPFH 正常微生物菌群对幽门螺杆菌 导致的体重下降有减缓作用 16 Duration(weeks)

RESULT——GFH小鼠体重低于GF小鼠 • in the absence of normal gut microbiota, H. pylori caused weight loss. • 正常微生物菌群对幽门螺杆菌 导致的体重下降有减缓作用