北京大学：《细胞生物学 Cell Science》课程教学资源（PPT课件讲稿）Cytoskeleton and motile of cells

10.6 Cytoskeleton and motile of cells Cell movements Whole cell movement; cell shaping; Intracellular movements; membrane fluidity 1. Whole cell movement )Swimming cilla and flagella F E 2)Locomotion -Amoeboid motion Ectoplasm( cortical zone) MF gelazation push and MF/myosin 3)Locomotion-fibroblastic movement 曰 Cell-substratum interaction and role of stress fibers Initial spreading: filopodia, ruffles, pseudopodia, lamellipodium ----MF Polarization ---MT and mToc Translocation----ME IF

10.6 Cytoskeleton and motile of cells Cell movements: Whole cell movement; cell shaping; Intracellular movements; membrane fluidity 1. Whole cell movement 1)Swimming cilla and flagella 2)Locomotion -Amoeboid motion Ectoplasm ( cortical zone ) MF gelazation push and MF/myosin 3)Locomotion-fibroblastic movement Cell-substratum interaction and role of stress fibers Initial spreading: filopodia, ruffles, pseudopodia, lamellipodium ----MF Polarization ---MT and MTOC Translocation---- MF, IF

2. Shaping--MF, MT, IF and the interaction of cytoskeleton with plasma membrane 3. Cell contractility )Muscle contraction-Sliding-filament hypothesis Sarcomere and myofibril Thick filament--- Myosin Thin filament--Actin filament Tropomyosin Troponin TnC. TnI TnT a-actinin titin 3 million

2. Shaping--MF, MT, IF and the interaction of cytoskeleton with plasma membrane 3. Cell contractility 1)Muscle contraction-sliding -filament hypothesis Sarcomere and myofibril Thick filament--- Myosin Thin filament--Actin filamemt Tropomyosin Troponin TnC, TnI TnT -actinin titin 3 million

The molecular basis of contraction myosin head rapidly bends toward the center of the sacomere, moving actin filament 5-15 nm Rigid"lever arm"of myosin neck 曰圆 The energetic of filament sliding---a model of the actinomyosin contractile cycle-ATP/ADP Excitation-contraction-coupling--Neuromusclar Junction The role of tropomyosin in muscle contracyion and Ca/troponin

The molecular basis of contraction myosin head rapidly bends toward the center of the sacomere, moving actin filament 5-15 nm Rigid "lever arm" of myosin neck The energetic of filament sliding---a model of the actinomyosin contractile cycle-ATP/ADP Excitation-contraction-coupling--Neuromusclar junction The role of tropomyosin in muscle contracyion and Ca2+/troponin

2)Nonmuscle contraction Stress fiber Actin/myosin Geled network 4. Mitosis and assembly and disassemblyof cytoskeleton Centrosome/ centroiles pindle Kinetochore Contractile ring

2)Nonmuscle contraction Stress fiber Actin/myosin Geled network 4. Mitosis and assembly and disassemblyof cytoskeleton Centrosome/ centroiles Spindle Cinetochore Contractile ring

D

3μ II HUVEC5 ul HUVEC7山 HUVEC10 HUVEC 0 Time s 24 Hour Time

Flagellum Mate Regenerating Intact Cytoplasm flagella flagella Ixes assembly

no Lun I oz

y

Direction of locomotion Power stroke Recovery stroke