北京化工大学：《生物工艺学》课程电子教案（PPT课件）Chapter 05 Reactor Design and Instrumentation

Chapter 5 Reactor Design and Instrumentation

Reactor Design and Instrumentation Chapter 5

PROMOTCS Exhaust air XANTHAN GUM treetment i 060 动 TISSUE CULTURE Liquid feeds Inoculum ANTITUMOR AGENTS CITRIC ACID reector 00酒0 Ammonia MONOCLONAL ANTIBODY Gucose 2 Disintegration MSG INOSINB Extraction 动前 VACCINES ⑧ 萌店 ANTIBIOTICS 动咖 WASTE WATER TREATMENT DIAG.ENZYMES 01i0 PEG Salt Phase separation SINGLE CELL PROTEIN .DNA 前动 PEG+ Air 的 harvest sterilisation Ultrafiltration STEROIDS IND.BNZYMES Product Buffer 0i的i2 前动 Freeze 0.01 0.1 1 10 drying 100 10001000100000 Bioreactor Size m3 The bioreactor and auxiliary equipment used for production of the intracellular enzyme B-galactosidase from E.coli 1g. Biotecholoy Products and Bioreactor Size

5.1.The conventional stirred tank reactor Dt Parameter Symbol Dimension Reactor diameter Dt Liquid height HL 2-5Dt Di Impeller diameter Di 0.33-0.5Dt Impeller distance I 1-1.5Di Baffle width Wb 0.08Dt △ Advantages of Impeller STR Baffle Sparger Air The conventional stirred tank reactor (STR)and some standard dimensions

5.1. The conventional stirred tank reactor Advantages of STR ?

Side view:Flow pattern: Top view: Disc turbine Open turbine 露 图 828 Propeller ☏ Common stirrer types used in bioreactors (Left hands figures illustrate the liquid flow pattern)

Common stirrer types used in bioreactors (Left hands figures illustrate the liquid flow pattern)

Some standard dimensions of the STR B0.2B D=T/3,H0=1.5-2.0T, 宝￥ B=0.1T,ho=0.25T, S=2-3D,C=D

D=T/3, H0=1.5-2.0T, B=0.1T, h0=0.25T, S=2-3D, C=D Some standard dimensions of the STR

5.2.The bubble column (tower)reactor 14 tAir out Gas out of power from air 40 Typical ower inut Typical Settling plates Airlift Loop htendtdhoogeneil nnnn ropariondpagIronit 0 homoeneity 80 Air inlet Advantages? 2/m3 Disadvantages? An aerobic bubble colum fermenter (left)andanaerobic tower Fig.4 Bubble and Air Lift Fermenier fermenter,here fitted with cell setting plate). 厌翼n

5.2. The bubble column (tower) reactor Advantages? Disadvantages?

5.3.The air lift reactor Air out Air out Air out Air in Air in Air in Advantages Air lift reactors

5.3. The air lift reactor Advantages ?

Advantages >Low energy requirement Not easily contaminated >High mass transfer

Advantages ➢ Low energy requirement ➢ Not easily contaminated ➢ High mass transfer

气体排出口 排气1 液面 10.8四 空气喷入管上 产品浪出管 气泡敏峰装置 19多孔档板 一降液部 一升液部 情 一换热器 空气入管 7 n 1111/ 0008 基质进口十 S opt3 p.p.m 一补充基成入口 1088 ..1 压差循环发酵器 冷斯管 O=93000Nm3l-J 压络空气一 空气吸入日 250t培养基h1 14-20 图8-1C1压力循环发静搬 T/16

5.4.The packed bed reactor and the fluidised bed reactor Medium inlet Anaerobic packed bed(left),aerobic packed bed(middle)and fluidised bed (right)reactors

5.4. The packed bed reactor and the fluidised bed reactor