《生物化学》课程PPT教学课件（留学生）Chapter 06 Bioenergetics and Oxidative Phosphorylation

BIOCHEMISTRYANDMOLECULARBIOLOGYChapter 6Bioenergetics andOxidative PhosphorylationDepartment of Biochemistry and Molecular Biology,Medicine School

Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY Chapter 6 Bioenergetics and Oxidative Phosphorylation

ABIOCHEMISTRYANDMOLECULARBIOLOGYOutline*Overview*Free energy change*Electron transport chain*Oxidativephosphorylation*ShuttleDepartment of Biochemistry and Molecular Biology,Medicine School

Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY Outline *Overview *Free energy change *Electron transport chain *Oxidative phosphorylation *Shuttle

GY特好力O#行region inanimatiormatrixintermermbranecytoplasmspaceDepartment of Biochemistry and Molecular Biology,Medicine School

Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY

BIOCHEMISTRYANDMOLECULARBIOLOGYMutationsin mtDNAare responsible for severaldiseases, including some cases of mitochondrialmyopathies,and Leber hereditary opticneuropathy,a disease in which bilateral loss ofcentral vision occurs as a result of neuro retinaldegeneration, including damage to the optic nerve.Department of Biochemistry and Molecular Biology,Medicine School

Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY Mutations in mtDNA are responsible for several diseases, including some cases of mitochondrial myopathies, and Leber hereditary optic neuropathy, a disease in which bilateral loss of central vision occurs as a result of neuro retinal degeneration, including damage to the optic nerve

山BIOCHEMISTRYANDMOLECULARBIOLOGY1.overviewBioenergetics describes the transfer andutilization of energy in biologic systems. Itmakes use of a few basic ideas from thefield of thermodynamics, particularly theconcept of free energy.Department of Biochemistry and Molecular Biology,Medicine School

Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY Bioenergetics describes the transfer and utilization of energy in biologic systems. It makes use of a few basic ideas from the field of thermodynamics, particularly the concept of free energy. 1. overview

BIOCHEMISTRYANDMOLECULARBIOLOGY2.Free energy changeIf G is negative number, the reaction goes spontaneouslysaid to be exergonic.If △G is positive number, the reaction does not gospontaneously, said to be endergonicIf △G is = O, the reactants are in equilibriumDepartment of Biochemistry and Molecular Biology,Medicine School

Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY If ΔG is negative number, the reaction goes spontaneously, said to be exergonic. If ΔG is positive number, the reaction does not go spontaneously, said to be endergonic. If ΔG is = 0, the reactants are in equilibrium. 2. Free energy change

BIOCHEMISTRYANDMOLECULARBIOLOGYATPasanenergycarrier.ATP consists of amolecule of adenosine towhich three phosphate groups are attached. Ifone phosphate is removed, adenosinediphosphate is produced; if two phosphates areremoved, adenosine monophosphate resultsDepartment of Biochemistry and Molecular Biology,Medicine School

Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY ATP as an energy carrier • ATP consists of a molecule of adenosine to which three phosphate groups are attached. If one phosphate is removed, adenosine diphosphate is produced; if two phosphates are removed, adenosine monophosphate results

BIOCHEMISTRYANDMOLECULARBIOLOGYVery high-energy phosphate compounds:phosphoenolpyruvate,H1,3-bisphosphoglycerate,N~ PphosphocreatineCNHO=CCOOHOHHCNP0C-CH2-CH2CCH21,3-COOHbisphosphoglphosphoenolpyruvatephosphocreatineycerateDepartment of Biochemistry and Molecular Biology,Medicine School

Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY Very high-energy phosphate compounds: phosphoenolpyruvate, 1,3-bisphosphoglycerate, phosphocreatine phosphoenolpyruvate COOH C CH2 O P 1,3- bisphosphogl ycerate O=C C OH CH2 O P O P phosphocreatine

BIOCHEMISTRYANDMOLECULARBIOLOGYATP as an intermediate in phosphate transfer:ATP occupies an intermediate position on thebioenergetic scale of phosphate containingcompounds.Department of Biochemistry and Molecular Biology,Medicine School

Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY ATP as an intermediate in phosphate transfer: ATP occupies an intermediate position on the bioenergetic scale of phosphate containing compounds

BIOCHEMISTRYANDMOLECULARBIOLOGY3.Electrontransport chainThe electron transport chain (respiratory chain):is pesent in the inner mitochondrial membrane and is thefinal common pathway by whichelectrons derived fromdifferent fuels of the body flow to oxygen.Fourcomplexcomplex INADH-CoQreductasecomplex IIsuccinate-CoQreductasecomplex IIICoQ-cytochromereductasecomplex IVcytochrome oxidaseDepartment of Biochemistry and Molecular Biology,Medicine School

Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY The electron transport chain (respiratory chain): is pesent in the inner mitochondrial membrane and is the final common pathway by which electrons derived from different fuels of the body flow to oxygen. 3. Electron transport chain Four complex NADH-CoQ reductase complex Ⅰ succinate- CoQ reductase complex Ⅱ CoQ-cytochrome reductase complex Ⅲ cytochrome oxidase complex Ⅳ