《转基因植物制作》（英文版） Methods of producing transgenic plants Part l

Methods of producing transgenic plants Part

Methods of producing transgenic plants Part II

Plant Biotechnology Traditional Recombinant crossbreeding DNA techniques Plasmid insertion resis Cutting sites RR FI Cross Mismatch Rr Mismatch Rr RR and Rr silt in F2 cross IRR: 2Rr: lrr Hie ratio k 3: 1 http://ww.insp.mx/xcongreso/ponencias/5

Plant Biotechnology •Traditional crossbreeding Recombinant DNA techniques http://www.insp.mx/xcongreso/ponencias/5

The results of traditional agbiotech are not that bad but they are time-consuming corn cob teosinte Wild tomato (Lycopersicon pimpinellifolium) D= 1 cm John Doebley photo

The results of traditional Agbiotech are not that bad, but they are time-consuming teosinte cob corn John Doebley photo Wild tomato (Lycopersicon pimpinellifolium ) D= 1 cm

Plant Breeding Process Selfing& Selecting Wild Domestic Germplasm Germplasm Elite Germplasm Single Othe Inbred Inbreds Commercial www.quirkyworks.com/design/samples/plant-breeding.jpg Hybrid

www.quirkyworks.com/design/ Samples/plant-breeding.jpg

Corn Yield(bulA)in Wisconsin Since 1866 200 1974 to 1998=1.6 bu A"yr"(HTy Hybrid plants 160 (heterosis 120 1960to1998=1.4buA'yr 80 1930to1959=1.3 bu A yr 40 1866to1929=0.0buA 0 1860 1880 9 1920 1940 1960 1980 2000

Hybrid plants (heterosis)

Agricultural Biotechnology crossbreeding Recombinant dna Species Related Related/unrelated Efficiency Susceptibility to external gene influences Genes shuffled in one 10sof1000s 1-few expriment Random insertion Insecti and herbicides Fertilizers Pollution/run off Potential toxins /risk 十 十 http://ww.insp.mx/xcongresolponencias/5

Agricultural Biotechnology Crossbreeding Recombinant DNA Species Related Related/unrelated Efficiency Susceptibility to external gene influences Genes shuffled in one exepriment 10s of 1000s 1 – few Random insertion + + Insecti and herbicides Fertilizers Pollution/run off Potential toxins/risk + + http://www.insp.mx/xcongreso/ponencias/5

Introducing genes into plants 1)infecting plant cells with plasmids as vectors carrying the desired gene Restriction Selectable enzyme site marker Restriction ORI enzyme site Restriction Restriction enzyme site enzyme site 2) shooting microscopic pellets containing the gene directly into the cell

Introducing genes into plants 1) infecting plant cells with plasmids as vectors carrying the desired gene; 2) shooting microscopic pellets containing the gene directly into the cell

Genetic engineering of plants with Agrobacterium tumefaciens A, tumefaciens, used extensively for genetic engineering of plants Contains T-DNA (bacterial plasmid) Genes colud be integrated into the plant chromosomes when the T-dna is Tumor induced by A. tumefaciens transferred http://courses.washingtonedu/z490/gmo/natural.html

Genetic engineering of plants with Agrobacterium tumefaciens • A. tumefaciens: used extensively for genetic engineering of plants. • Contains T-DNA (bacterial plasmid) • Genes colud be integrated into the plant chromosomes when the T-DNA is transferred. http://courses.washington.edu/z490/gmo/natural.html Tumor induced by A. tumefaciens

A Tumefaciens gall is not a tiny thing teide P profed E copyright: IFFF(BOKU)@ 1996 Reg dtu f califor

A.Tumefaciens gall is not a tiny thing

Biology of A tumefaciens Well known to induce crown gall tumor A tumefaciens lives around root surfaces(in rhizosphere) where it using nutriel that leak from the root tissues 谷 infects only through wound sites and actively chemotactic to them Www-genvagar.slu.se/teknik/ djup/plasm. htm Bacterial T-plasmid produces Plant wound produces receptors for acetosyringone COCH acetosyringone CH, O OCH

Biology of A. tumefaciens www-genvagar.slu.se/teknik/ djup/plasm.htm Well known to induce crown gall tumor A.tumefaciens lives around root surfaces (in rhizosphere) where it using nutrients that leak from the root tissues infects only through wound sites and actively chemotactic to them Plant wound produces acetosyringone Bacterial T-plasmid produces receptors for acetosyringone