武汉大学生命科学学院：《分子生物学》英文版 Chapter 6-15 Key points

Key points for Chapter 6 1. Definitions: topoisomerases ribozyme, double helix, DNA denaturation, Tm, linking number, pseudoknot. 2 What are the structural differences between dna and rna? how the structural properties of dNA and rna determine their distinct biological functions

Key points for Chapter 6 1.Definitions: topoisomerase, ribozyme, double helix, DNA denaturation, Tm, linking number, pseudoknot. 2.What are the structural differences between DNA and RNA? How the structural properties of DNA and RNA determine their distinct biological functions

Key points for Chapter 7 1. Definitions: nucleosome, gene density, core histones(structure and function), Nucleosome remodeling complexes 2, Describe the important functions of packing of dNA into chromosome 3. Why genes make up only a small proportion of the eukaryotic genome

Key points for Chapter 7 1.Definitions: nucleosome, gene density, core histones (structure and function), Nucleosome remodeling complexes 2.Describe the important functions of packing of DNA into chromosome. 3.Why genes make up only a small proportion of the eukaryotic genome

Key points for Chapter 7 4. Briefly describe roles of three critical dNA elements important for chromosome duplication segregation 5. Briefly describe how the higher. order chromatin structure is formed

Key points for Chapter 7 4.Briefly describe roles of three critical DNA elements important for chromosome duplication & segregation 5.Briefly describe how the higher￾order chromatin structure is formed

Key points for Chapter 8 1. Definitions: replication fork, leading strand, lagging strand, okazaki fragment, processivity, proofreading exonuclease, replisome, pre- replicative complexes (pre-RCS) 2 Describe the function and mechanism of dNa polymerase. 3. Describe DNA replication process and proteins involved at a replication fork

Key points for Chapter 8 1.Definitions: replication fork, leading strand, lagging strand, Okazaki fragment, processivity, proofreading exonuclease, replisome, pre￾replicative complexes (pre-RCs) 2.Describe the function and mechanism of DNA polymerase. 3.Describe DNA replication process and proteins involved at a replication fork

Key points for Chapter 8 4. How is the dNA replication tightly controlled in E coand in eukaryotic cells? 5. What is the end replication problem? how does cell resolve the problem?

Key points for Chapter 8 4.How is the DNA replication tightly controlled in E. coli and in eukaryotic cells? 5.What is the end replication problem? how does cell resolve the problem?

Key points for Chapter 9 1. Definitions: replication errors, spontaneous dNA damage, DNA mutations double-strand break (DSB)repair pathway. 2.H。wd。 es the mismatch repair system accurately detect, remove and repair the mismatch resulting from inaccurate replication? 3 What are the environmental factors that cause DNA damage?

Key points for Chapter 9 1.Definitions: replication errors, spontaneous DNA damage, DNA mutations, double-strand break (DSB) repair pathway. 2.How does the mismatch repair system accurately detect, remove and repair the mismatch resulting from inaccurate replication? 3.What are the environmental factors that cause DNA damage?

Key points for Chapter 9 4. How could a dna damage be converted to dNa mutation? 5. What are the mechanisms to repair DNA damage? Describes how base excision and repair and nucleotide excision repair work? 6. What is translesion DNA synthesis? Why it is important?

Key points for Chapter 9 4.How could a DNA damage be converted to DNA mutation? 5.What are the mechanisms to repair a DNA damage? Describes how base excision and repair and nucleotide excision repair work? 6.What is translesion DNA synthesis? Why it is important?

Key points for Chapter 10 1. Definitions: Mating-type switching, gene conversion, Holliday junction 2. Compare the two models for homologous recombination, which model finds more evidence? 3. Describe RecBCD pathway and protein involved in bacteria, and the function of the eukaryotic homologue Spo11, MRX and dmc1

Key points for Chapter 10 1.Definitions: Mating-type switching, gene conversion, Holliday junction 2.Compare the two models for homologous recombination, which model finds more evidence? 3.Describe RecBCD pathway and protein involved in bacteria, and the function of the eukaryotic homologue Spo11, MRX and Dmc1

Key points for Chapter 11 1. Conservative site-specific recombination(CSSR: definition consequence, mechanism and examples 2. Transposon and transposition: definitions, consequence, basic structural feature of three principle classes of transposable elements, and the mechanism of viral-like retrotransposons/retroviruses

Key points for Chapter 11 1.Conservative site-specific recombination (CSSR): definition, consequence, mechanism and examples. 2.Transposon and transposition: definitions, consequence, basic structural feature of three principle classes of transposable elements, and the mechanism of viral-like retrotransposons/retroviruses

Key points for Chapter 12 1. The central dogma, 2. Transposon and transposition: definitions, consequence, basic structural feature of three principle classes of transposable elements, and the mechanism of viral-like retrotransposons/retroviruses

Key points for Chapter 12 1.The central dogma, 2.Transposon and transposition: definitions, consequence, basic structural feature of three principle classes of transposable elements, and the mechanism of viral-like retrotransposons/retroviruses