武汉大学生命科学学院：《分子生物学》（英文版）Welcome Each of You to My Molecular Biology Class

Welcome each of You to My olecular Biology class @博来字 Wuhan University

Welcome Each of You to My Molecular Biology Class

Molecular Biology of the Gene 5/E --Watson et al. (2004) Part I: Chemistry and Genetics Part maintenance of the genome Part l: Expression of the Genome Part IV: Regulation Part V: Methods Wuhan University

2 Molecular Biology of the Gene, 5/E --- Watson et al. (2004) Part I: Chemistry and Genetics Part II: Maintenance of the Genome Part III: Expression of the Genome Part IV: Regulation Part V: Methods

The revised central dogma2008 Ch o The siructures or DNA and rNa Ch 8: The replication of DNA 基因组的保持 人 M DNA Replication Reverse transcript ol Transcript on RNA PI rocessing 八八八RNA Translation 基因组的表达 Gene regulation +13 Protein

The revised central dogma2008 RNA processing Gene regulation Ch 6: The structures of DNA and RNA 基 Ch 8: The replication of DNA 因 组 的 保 持 基 因 组 的 表 达

Part l: Expression of the Genome Ch 12: Mechanisms of transcription Ch 13: RNA splicing Ch 14: Translation Ch 15: The genetic code

4 Ch 12: Mechanisms of transcription Ch 13: RNA splicing Ch 14: Translation Ch 15: The genetic code Part III: Expression of the Genome

Molecular iolod course Chapter 12: Mechanisms of Transcription of the (rene 1. RNA polymerase and the transcription cycle 2. The transcription cycle in bacteria WATSON BAKER 3. Transcription in eukaryotes 4. Transcription by rNa polymerase I and III

Chapter 12: Mechanisms of Transcription 1. RNA polymerase and the transcription cycle 2. The transcription cycle in bacteria 3. Transcription in eukaryotes 4. Transcription by RNA polymerase I and III Molecular Biology Course

Transcription vs replication Transcription is chemically and enzymatically very similar to DNA replication

Transcription vs replication 6 Transcription is chemically and enzymatically very similar to DNA replication

Some important differencesi 1 RNA is made of ribonucleotides 2. RNA polymerase catalyzes the reaction, which does not require a primer(de novo synthesis) 3. The RNA product does not remain base-paired to the template DNA strand 4. Less accurate (error rate: 10-4)

7 Some important differences: 1.RNA is made of ribonucleotides 2.RNA polymerase catalyzes the reaction, which does not require a primer (de novo synthesis) 3.The RNA product does not remain base-paired to the template DNA strand 4.Less accurate (error rate: 10-4)

5. Transcription selectively copies only certain parts of the genome and makes one to several hundred or even thousand copies of any given section of the genome(Replication?)

8 5.Transcription selectively copies only certain parts of the genome and makes one to several hundred, or even thousand, copies of any given section of the genome. (Replication?)

DNA duplex AITI RNA Transcription bubble Fig 12-1 Transcription of DNA into RNA

9 Fig 12-1 Transcription of DNA into RNA Transcription bubble

CHHAPTER12: Mechanisms of transcription Topic 1: RNA Polymerase and The Transcription Cycle See the interactive animation Wuhan universit

10 Topic 1: RNA Polymerase and The Transcription Cycle CHAPTER12: Mechanisms of Transcription See the interactive animation