武汉大学生命科学学院：《分子生物学》（英文版）Welcome Each of You to My Molecular Biology Class

Welcome each of You to My olecular Biology class @博来字 Wuhan University

Welcome Each of You to My Molecular Biology Class

Molecular Biology of the Gene 5/E --Watson et al. (2004) Part I: Chemistry and Genetics Part: Maintenance of the genome Part ll: Expression of the Genome Part IV: Regulation Part v: methods Wuhan University

Molecular Biology of the Gene, 5/E --- Watson et al. (2004) Part I: Chemistry and Genetics Part II: Maintenance of the Genome Part III: Expression of the Genome Part IV: Regulation Part V: Methods

Part ll: Maintenance of the genome Dedicated to the structure of DNA and the processes that propagate,maintain and alter it from one cell generation to the next @博字 Wuhan University

Part II: Maintenance of the Genome Dedicated to the structure of DNA and the processes that propagate, maintain and alter it from one cell generation to the next

Ch 6: The structures of dna and rna Ch 7: Chromosomes, chromatins and the nucleosome Ch 8: The replication of DNA Ch 9: The mutability and repair of DNA Ch 10: Homologous recombination at the molecular level Ch 11: Site-specific recombination and transposition of DNA

Ch 6: The structures of DNA and RNA Ch 7: Chromosomes, chromatins and the nucleosome Ch 8: The replication of DNA Ch 9: The mutability and repair of DNA Ch 10: Homologous recombination at the molecular level Ch 11: Site-specific recombination and transposition of DNA

Molecular iology Course CHAPTER 8: The replication Of dna direction of leading strand polymerase movement leading strand overall direction of . TA1DA AWDA4 DNA replication RNA primers DNA polymerse kazak fragments lagging strand direction of lagging strand polymerase movement replicated DNA unreplicated DNA @博来字 Wuhan Universit

CHAPTER 8: The replication of DNA •Molecular Biology Course

CHAPTER& The replication of nA Teaching Arrangement Watch animation-Understand replication a Go through some structural tutorial-Experience the BEAUTY of the DNA polymerase a Lecture-comprehensive understanding and highlight Key points

Teaching Arrangement ◼ Watch animation-Understand replication ◼ Go through some structural tutorial-Experience the BEAUTY of the DNA polymerase ◼ Lecture-comprehensive understanding and highlight Key points CHAPTER 8 The replication of DNA

CHAPTER& The replication of nA The chemistry of DNA Synthesis The Mechanism of DNA Polymerase > The Specialization of DNA Reaction Polymerases catalyst >The Replication Fork Process DNA Synthesis at the Replication Fork >Initiation of DNA Replication Initiation Binding and Unwinding Termination >Finishing Replication

➢ The Chemistry of DNA Synthesis ➢ The Mechanism of DNA Polymerase ➢ The Specialization of DNA Polymerases ➢ The Replication Fork ➢ DNA Synthesis at the Replication Fork ➢ Initiation of DNA Replication ➢ Binding and Unwinding ➢ Finishing Replication Process Initiation & Termination CHAPTER 8 The replication of DNA Reaction & Catalyst

CHAPTER& The replication of nA The first part describes the basic chemistry of DNA synthesis and the function of the dna polymerase @博共字 Wuhan Universit

The first part describes the basic chemistry of DNA synthesis and the function of the DNA polymerase CHAPTER 8 The replication of DNA

CHAPTER& The replication of nA The chemistry of DNA DNA synthesis requires deoxynucleoside triphosphates and a primer:template junction DNA is synthesized by extending the 3 end of the primer Hydrolysis of pyrophosphate(PPi is the driving force for dNA synthesis @博共字 Wuhan Universit

The Chemistry of DNA CHAPTER 8 The replication of DNA ➢ DNA synthesis requires deoxynucleoside triphosphates and a primer:template junction ➢ DNA is synthesized by extending the 3’ end of the primer ➢ Hydrolysis of pyrophosphate (PPi) is the driving force for DNA synthesis

base(A, G, C, or T) Ho—P—0-P-—P—0-CH2 OH b annealed primer growing end of DNA 50 OH 3 template strand ACGT CGACGTCGACGTCGACGT 3 HO 5 dsDNA SSDNA Figure 8-3 Substrates required for DNA synthesis

Figure 8-3 Substrates required for DNA synthesis