哈佛大学：《分子生物学》课程PPT教学课件（英文版）Announcements

Announcements Power point lecture notes available on bcmp200 Website For further reading Nucleic Acids: Structures, Properties, and Functions vA Bloomfield D.M. Crothers Ⅰ. Tinoco

For further reading: Nucleic Acids: Structures, Properties, and Functions V.A. Bloomfield D.M. Crothers I. Tinoco Announcements: Power Point Lecture Notes Available on BCMP200 Website

Interference Footprinting DNA+MMS● Cleave backbone a modified bases saturating Protein] ) ★ Gel shift

Interference Footprinting DNA + MMS + Saturating [Protein] Gel Shift 14 Cleave backbone @ modified bases

Chromatin is the last refuge of scoundrels

“Chromatin is the last refuge of scoundrels

A Mitotic human chromosome DNA length:4.6x 10 bp=1.5 cm Chromosome length: 2 um Compaction ratio=8000

A Mitotic Human Chromosome DNA length: 4.6 x 107 bp = 1.5 cm Chromosome length: 2 µm Compaction ratio = 8000 1

Chromatin DNA DNA Compaction Chromatin Accessibility Remodeling Machines NIH

Chromatin DNA Compaction DNA Chromatin Accessibility Remodeling Machines NIH

Basic Unit of Chromatin Structure: Polynucleosome Compaction ratio=6 2

Basic Unit of Chromatin Structure: Polynucleosome Compaction ratio = 6 2

Limited Micrococcal Nulcease Digestion of Chromatin Generates a 200 bp ladder 605→ 405→ 205→ 3

Limited Micrococcal Nulcease Digestion of Chromatin Generates a ~200 bp Ladder 3

Operational Definititions of Nucleosome Particles Linker DNA(variable) H HI H 200bp 165bp 146bp HI HI Chromatin Mononucleosome Chromatosome Core Particle Micrococcal Nuclease Digestion Time

H1 H1 H1 H1 H1 H1 Micrococcal Nuclease Digestion Time Mononucleosome Chromatosome Core Particle Linker DNA (variable) Operational Definititions of Nucleosome Particles Chromatin 4

The 30 nm Chromatin fiber Compaction ratio =50

The 30 nm Chromatin Fiber Compaction ratio = 50 5

A Mitotic human chromosome DNA length:4.6x 10 bp=1.5 cm Chromosome length: 2 um 30 nm filament Compaction ratio=8000

A Mitotic Human Chromosome DNA length: 4.6 x 107 bp = 1.5 cm Chromosome length: 2 µm Compaction ratio = 8000 1 30 nm filament