《医学遗传学 Medical Genetics》课程PPT教学课件（英文版）Gene Manipulation

Medical Genetics Gene manipulation

Gene Manipulation Medical Genetics

Recombination DNA Technique Techniques for joining DNA molecules in vitro and introducing them into living cells where they replicate. &o Insert specific DNA into vector Clone in host cells go Cloning ☆ Isolate cloned dna

Recombination DNA Technique Techniques for joining DNA molecules in vitro and introducing them into living cells where they replicate. ❖ Insert specific DNA into vector ❖ Clone in host cells ❖ Cloning ❖ Isolate cloned DNA

TTTT L foreign dna bacterialcell Schematic of recombination DNa technique

foreign DNA bacterial cell Schematic of recombination DNA technique

foreign dna bacterialcell restriction endonuclease Schematic of recombination DNa technique

vector restriction endonuclease foreign DNA bacterial cell Schematic of recombination DNA technique

foreign dna bacterialcell restriction endonuclease ligase ◎ Schematic of recombination DNa technique host

ligase vector restriction endonuclease foreign DNA bacterial cell host Schematic of recombination DNA technique

foreign dna bacterialcell restriction endonuclease ligase ◎ Schematic of recombination DNa technique host

ligase vector restriction endonuclease foreign DNA bacterial cell host Schematic of recombination DNA technique

Cloning Schematic of recombination DNa technique

Schematic of recombination DNA technique Cloning

Blue-white screening Am np Amp+X-gal Schematic of recombination DNa technique

Amp Amp＋X-gal Schematic of recombination DNA technique “Blue-white” screening

Recombination dna ☆ DNA clone 令 CDNA CIone 8 Artificial gene clone 冷 PCR product clone

Recombination DNA ❖ DNA clone ❖ cDNA clone ❖ Artificial gene clone ❖ PCR product clone

Gene Transfer Transgenic animal P Transgenic mouse Pu Embryonic stem cell, ES cell

Gene Transfer ❖ Transgenic animal  Transgenic mouse  Embryonic stem cell，ES cell